Background Techniques for the fast and accurate detection of bacterial infection are critical for early diagnosis, prevention and treatment of bacterial translocation in clinical severe acute pancreatitis （SAP）. In ...Background Techniques for the fast and accurate detection of bacterial infection are critical for early diagnosis, prevention and treatment of bacterial translocation in clinical severe acute pancreatitis （SAP）. In this study, the availability of a real-time PCR method in detection of bacterial colonization in SAP rat models was investigated. Methods Samples of blood, mesenteric lymph nodes （MLN）, pancreas and liver from 24 specific pathogen-free rats （8 in a control group, 16 in a SAP group） were detected for bacterial infection rates both by agar plate culture and a real-time PCR method, and the results were made contrast. Results Bacterial infection rates of the blood, MLN, pancreas and liver in the SAP group and the control group by the two different methods were almost the same, which were 5/16, 12/16, 15/16, 12/16 in the SAP group compared with 0/8, 1/8, 0/8, 0/8 in the control group by agar plate culture, while 5/16, 10/16, 13/16, 12/16 and 0/8, 1/8, 0/8, 0/8 respectively by a real-time PCR method. Bacterial number was estimated by real-time PCR, which showed that in the same mass of tissues, the pancreas contained more bacteria than the other three kinds of organs in SAP rats （P 〈0.01）, that may be due to the edema, necrosis and hemorrhage existing in the pancreas, making it easier for bacteria to invade and breed. Conclusion Fast and accurate detection of bacterial translocation in SAP rat models could be carried out by a real-time PCR procedure.展开更多
Objective:To evaluate the efficacy and safety of Wuda Granule(WDG)on recovery of gastrointestinal function after laparoscopic bowel resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative...Objective:To evaluate the efficacy and safety of Wuda Granule(WDG)on recovery of gastrointestinal function after laparoscopic bowel resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative care.Methods:A total of 108 patients aged 18 years or older undergoing laparoscopic bowel resection with a surgical duration of 2 to 4.5 h were randomly assigned(1:1)to receive either WDG or placebo(10 g/bag)twice a day from postoperative days 1-3,combining with ERAS-based perioperative care.The primary outcome was time to first defecation.Secondary outcomes were time to first flatus,time to first tolerance of liquid or semi-liquid food,gastrointestinal-related symptoms and length of stay.Subgroup analysis of the primary outcome according to sex,age,tumor site,surgical time,histories of underlying disease or history of abdominal surgery was undertaken.Adverse eventse were observed and recorded.Results:A total of 107patients[53 in the WDG group and 54 in the placebo group;61.7±12.1 years;50 males(46.7%)]were included in the intention-to-treat analysis.The patients in the WDG group had a significantly shorter time to first defecation and flatus[between-group difference-11.01 h(95%CI-20.75 to-1.28 h),P=0.012 for defecation;-5.41 h(-11.10 to 0.27 h),P=0.040 for flatus]than the placebo group.Moreover,the extent of improvement in postoperative gastrointestinal-related symptoms in the WDG group was significantly better than that in the placebo group(P<0.05).Subgroup analyses revealed that the benefits of WDG were significantly superior in patients who were male,or under 60 years old,or surgical time less than 3 h,or having no history of basic disease or no history of abdominal surgery.There were no serious adverse events.Conclusion:The addition of WDG to an ERAS postoperative care may be a viable strategy to enhance gastrointestinal function recovery after laparoscopic bowel resection surgery.(Registry No.ChiCTR2100046242)展开更多
基金This work was supported by a grant from the Natural Science Foundation of Guangdong Province, China （No. 2007A07001680）.
文摘Background Techniques for the fast and accurate detection of bacterial infection are critical for early diagnosis, prevention and treatment of bacterial translocation in clinical severe acute pancreatitis （SAP）. In this study, the availability of a real-time PCR method in detection of bacterial colonization in SAP rat models was investigated. Methods Samples of blood, mesenteric lymph nodes （MLN）, pancreas and liver from 24 specific pathogen-free rats （8 in a control group, 16 in a SAP group） were detected for bacterial infection rates both by agar plate culture and a real-time PCR method, and the results were made contrast. Results Bacterial infection rates of the blood, MLN, pancreas and liver in the SAP group and the control group by the two different methods were almost the same, which were 5/16, 12/16, 15/16, 12/16 in the SAP group compared with 0/8, 1/8, 0/8, 0/8 in the control group by agar plate culture, while 5/16, 10/16, 13/16, 12/16 and 0/8, 1/8, 0/8, 0/8 respectively by a real-time PCR method. Bacterial number was estimated by real-time PCR, which showed that in the same mass of tissues, the pancreas contained more bacteria than the other three kinds of organs in SAP rats （P 〈0.01）, that may be due to the edema, necrosis and hemorrhage existing in the pancreas, making it easier for bacteria to invade and breed. Conclusion Fast and accurate detection of bacterial translocation in SAP rat models could be carried out by a real-time PCR procedure.
基金Supported by the Special Subsidy for Clinical Research of Guangdong Provincial Hospital of Chinese Medicine(No.YN10101902)the Double First-Class and High-Level University Discipline Collaborative Innovation Team Project of Guangzhou University of Chinese Medicine(No.2021xk48)。
文摘Objective:To evaluate the efficacy and safety of Wuda Granule(WDG)on recovery of gastrointestinal function after laparoscopic bowel resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative care.Methods:A total of 108 patients aged 18 years or older undergoing laparoscopic bowel resection with a surgical duration of 2 to 4.5 h were randomly assigned(1:1)to receive either WDG or placebo(10 g/bag)twice a day from postoperative days 1-3,combining with ERAS-based perioperative care.The primary outcome was time to first defecation.Secondary outcomes were time to first flatus,time to first tolerance of liquid or semi-liquid food,gastrointestinal-related symptoms and length of stay.Subgroup analysis of the primary outcome according to sex,age,tumor site,surgical time,histories of underlying disease or history of abdominal surgery was undertaken.Adverse eventse were observed and recorded.Results:A total of 107patients[53 in the WDG group and 54 in the placebo group;61.7±12.1 years;50 males(46.7%)]were included in the intention-to-treat analysis.The patients in the WDG group had a significantly shorter time to first defecation and flatus[between-group difference-11.01 h(95%CI-20.75 to-1.28 h),P=0.012 for defecation;-5.41 h(-11.10 to 0.27 h),P=0.040 for flatus]than the placebo group.Moreover,the extent of improvement in postoperative gastrointestinal-related symptoms in the WDG group was significantly better than that in the placebo group(P<0.05).Subgroup analyses revealed that the benefits of WDG were significantly superior in patients who were male,or under 60 years old,or surgical time less than 3 h,or having no history of basic disease or no history of abdominal surgery.There were no serious adverse events.Conclusion:The addition of WDG to an ERAS postoperative care may be a viable strategy to enhance gastrointestinal function recovery after laparoscopic bowel resection surgery.(Registry No.ChiCTR2100046242)
