Objective：To evaluate the in vitro and in vivo toxicity of self-prepared nanosized Fe2O3, which has the potential implication in tumor hyperthermia. Methods： Fe2O3 nanoparticles were prepared by improving co-precipi...Objective：To evaluate the in vitro and in vivo toxicity of self-prepared nanosized Fe2O3, which has the potential implication in tumor hyperthermia. Methods： Fe2O3 nanoparticles were prepared by improving co-precipitation, which characterization was detected by TEM, XRD, CMIAS, EDS. MTT assay was used to evaluate the in vitro cytotoxicity test; hemolytic test was carried out to estimate whether it has blood toxicity; Fe2O3 suspended in sterile 0.9% NaCl was intraperitoneally injected into Kumning mouse to calculate the LD50 ; micronucleus （MN） were reckoned to identify whether it is genotoxic. Results：The nanoparticles are brown spherical particles with diameter ranging from 8 to 15 nm, which have good decentralization and stability. The experiments also showed that the toxicity of the material on mouse fibroblast （L-929） cell lines was 0 - 1 degree ; it has no hemolysis activity; LD50 arrived at 5.45 g/kg^-1 after intraperitoneal injection of 1 ml suspension; micronucleus test showed that it has no genotoxic effects either. Conclusion： The results showed that the Fe2O3 nanoparticles are prepared successfully, the self-prepared nanosized Fe2O3 is a kind of high biocompatibility materials and perhaps it is suitable for further application in tumor hyperthermia.展开更多
Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in mice.Methods According to a random number table,40 mice were randomly divided into negative control gro...Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in mice.Methods According to a random number table,40 mice were randomly divided into negative control group(0.5%carboxymethylcellulose sodium,20 mL/kg),positive control group(0.1%mixture of carbon tetrachloride in soybean oil,20 mL/kg),RK group(the ethyl acetate extracts of RK,250 g crude drug/kg)and VRK group(the ethyl acetate extracts of VRK,250 g crude drug/kg)with 10 mice per group.All mice were administered orally by gavage daily for 7 continuous days.The morphology of liver tissues was examined to assess the liver injury by a transmission electron microscope.Hepatocyte apoptosis in vivo was determined by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nickend labeling(TUNEL)assay.Immunohistochemical technique was adopted to detect the expression of particular antiapoptotic and proapoptotic proteins in the mitochondrial pathways,including B-cell lymphoma(Bcl-2)and caspase-3,as well as the expression of inflammatory mediators,including nuclear factor kappa B(NF-κB)and intercellular adhesion molecule-1(ICAM-1).Results Liver injury and hepatocyte apoptosis were observed in RK mice,and the liver injury were significantly reduced in VRK-treated mice.In immunohistochemistry study,compared with the negative control group,RK inhibited dramatically the Bcl-2 protein expression and significantly increased the expression of caspase-3,NF-κB and ICAM-1(all P<0.01).Compared with the RK group,VRK group induced significant increase on Bcl-2 protein expression,and decreased the caspase-3,NF-κB and ICAM-1 protein expression(P<0.05 or <0.01).Conclusion The mechanism of reduced hepatotoxicity of VRK may be associated with the reduced inflammation,regulation of antiapoptotic and proapoptotic mediators in the mitochondrial pathway.展开更多
基金Grant sponsor:National Natural Science Foundation of China,Grant number:30371830Grant sponsor:National Hi-tech research and development program of China,Grant number:2002AA302207+3 种基金 Grant sponsor:Natural Science Foundation of Jiangsu,Grant number:BK2001003Grant sponsor:Hi-tech research pro-gram of Jiangsu,Grant number:BG2001006 Grant sponsor:Key Project of Chinese Traditional Medicine of Jiangsu,Grant number:H027Grant sponsor:Sci-ence Foundation of Southeast University,Grant number:9223001162
文摘Objective：To evaluate the in vitro and in vivo toxicity of self-prepared nanosized Fe2O3, which has the potential implication in tumor hyperthermia. Methods： Fe2O3 nanoparticles were prepared by improving co-precipitation, which characterization was detected by TEM, XRD, CMIAS, EDS. MTT assay was used to evaluate the in vitro cytotoxicity test; hemolytic test was carried out to estimate whether it has blood toxicity; Fe2O3 suspended in sterile 0.9% NaCl was intraperitoneally injected into Kumning mouse to calculate the LD50 ; micronucleus （MN） were reckoned to identify whether it is genotoxic. Results：The nanoparticles are brown spherical particles with diameter ranging from 8 to 15 nm, which have good decentralization and stability. The experiments also showed that the toxicity of the material on mouse fibroblast （L-929） cell lines was 0 - 1 degree ; it has no hemolysis activity; LD50 arrived at 5.45 g/kg^-1 after intraperitoneal injection of 1 ml suspension; micronucleus test showed that it has no genotoxic effects either. Conclusion： The results showed that the Fe2O3 nanoparticles are prepared successfully, the self-prepared nanosized Fe2O3 is a kind of high biocompatibility materials and perhaps it is suitable for further application in tumor hyperthermia.
基金the National Natural Science Foundation of China(No.81673599,81503250,and 21705081)the Natural Science Research Program of Jiangsu Higher Education Institutions(No.15KJB360009)+2 种基金the Natural Science Foundation of Jiangsu Province(No.BK20161037)the Project Foundation of the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.PAPD-2014)Six Talent Peaks Program of Jiangsu Province(No.2016-YY-026)。
文摘Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in mice.Methods According to a random number table,40 mice were randomly divided into negative control group(0.5%carboxymethylcellulose sodium,20 mL/kg),positive control group(0.1%mixture of carbon tetrachloride in soybean oil,20 mL/kg),RK group(the ethyl acetate extracts of RK,250 g crude drug/kg)and VRK group(the ethyl acetate extracts of VRK,250 g crude drug/kg)with 10 mice per group.All mice were administered orally by gavage daily for 7 continuous days.The morphology of liver tissues was examined to assess the liver injury by a transmission electron microscope.Hepatocyte apoptosis in vivo was determined by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nickend labeling(TUNEL)assay.Immunohistochemical technique was adopted to detect the expression of particular antiapoptotic and proapoptotic proteins in the mitochondrial pathways,including B-cell lymphoma(Bcl-2)and caspase-3,as well as the expression of inflammatory mediators,including nuclear factor kappa B(NF-κB)and intercellular adhesion molecule-1(ICAM-1).Results Liver injury and hepatocyte apoptosis were observed in RK mice,and the liver injury were significantly reduced in VRK-treated mice.In immunohistochemistry study,compared with the negative control group,RK inhibited dramatically the Bcl-2 protein expression and significantly increased the expression of caspase-3,NF-κB and ICAM-1(all P<0.01).Compared with the RK group,VRK group induced significant increase on Bcl-2 protein expression,and decreased the caspase-3,NF-κB and ICAM-1 protein expression(P<0.05 or <0.01).Conclusion The mechanism of reduced hepatotoxicity of VRK may be associated with the reduced inflammation,regulation of antiapoptotic and proapoptotic mediators in the mitochondrial pathway.
