Background Nontuberculous mycobacterium （NTM） had been reported to cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. Among NTM infections, Mycobacteri...Background Nontuberculous mycobacterium （NTM） had been reported to cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. Among NTM infections, Mycobacterium marinum （M. marinum） are mostly seen to cause skin infection. It is therefore important to establish a rapid approach for detection and identification of M. marinum from lesions of patients with suspected M. marinum infections. Methods Specimens were obtained from 5 patients with swimming pool granuloma. DNA was extracted and polymerase chain reaction （PCR） was performed. PCR products were digested with Hae III and BstE II, then analysed by pattern restriction analysis to detect heat shock protein （hsp） 65 kD gene. Results The 65 kD hsp gene was found in all specimens from patients with swimming pool granuloma. PCR restriction analysis （PRA） identified all 5 samples to be M. marinum infections, and the result was consistent with that of routine bacteriological identification. The lesions subsided or markedly improved upon treatment. Conclusions PRA is a sensitive, specific and rapid method in identification of mycobacteria. Application of this method will be helpful for early diagnosis of mycobacterial skin infections.展开更多
The situation of tuberculosis （TB） and Mycobacterium tuberculosis （ M. tuberculosis） drug resistance is still very serious in China. Improper treatment for TB may lead to a prolonged course of antimicrobial therap...The situation of tuberculosis （TB） and Mycobacterium tuberculosis （ M. tuberculosis） drug resistance is still very serious in China. Improper treatment for TB may lead to a prolonged course of antimicrobial therapy and spreading of drug resistant strains. Thus, rapid detection of drug resistant strains will allow prompt initiation and adjustment of effective chemotherapeutic treatment of TB. Ethambutol （EMB） is a first line anti-TB drug. embB Met306 is located in a cytoplasmic loop that forms an EMB resistance determining region,展开更多
The situation of tuberculosis and M. tuberculosis （MTB） drug resistance is severe in China.Among the MTB isolates, 27.8% were resistant to one primary antituberculosis drug at least. Isoniazid （INH） is one of the ...The situation of tuberculosis and M. tuberculosis （MTB） drug resistance is severe in China.Among the MTB isolates, 27.8% were resistant to one primary antituberculosis drug at least. Isoniazid （INH） is one of the first-line anti-TB drugs. The rates of primary resistance and acquired resistance to INH were 11.0% and 31.0%, respectively. MTB strains resistance to INH is mainly caused by the alterations in several genes encoding the molecular targets as follows： catalase peroxidase （katG）,展开更多
文摘Background Nontuberculous mycobacterium （NTM） had been reported to cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. Among NTM infections, Mycobacterium marinum （M. marinum） are mostly seen to cause skin infection. It is therefore important to establish a rapid approach for detection and identification of M. marinum from lesions of patients with suspected M. marinum infections. Methods Specimens were obtained from 5 patients with swimming pool granuloma. DNA was extracted and polymerase chain reaction （PCR） was performed. PCR products were digested with Hae III and BstE II, then analysed by pattern restriction analysis to detect heat shock protein （hsp） 65 kD gene. Results The 65 kD hsp gene was found in all specimens from patients with swimming pool granuloma. PCR restriction analysis （PRA） identified all 5 samples to be M. marinum infections, and the result was consistent with that of routine bacteriological identification. The lesions subsided or markedly improved upon treatment. Conclusions PRA is a sensitive, specific and rapid method in identification of mycobacteria. Application of this method will be helpful for early diagnosis of mycobacterial skin infections.
基金This research was supported by the Army Research Foundation forthe Outstanding Person with Ability (No.01J020).
文摘The situation of tuberculosis （TB） and Mycobacterium tuberculosis （ M. tuberculosis） drug resistance is still very serious in China. Improper treatment for TB may lead to a prolonged course of antimicrobial therapy and spreading of drug resistant strains. Thus, rapid detection of drug resistant strains will allow prompt initiation and adjustment of effective chemotherapeutic treatment of TB. Ethambutol （EMB） is a first line anti-TB drug. embB Met306 is located in a cytoplasmic loop that forms an EMB resistance determining region,
基金This study was supported by the a grant from Army Research Foundation for Outstanding Person (No. 01J020).
文摘The situation of tuberculosis and M. tuberculosis （MTB） drug resistance is severe in China.Among the MTB isolates, 27.8% were resistant to one primary antituberculosis drug at least. Isoniazid （INH） is one of the first-line anti-TB drugs. The rates of primary resistance and acquired resistance to INH were 11.0% and 31.0%, respectively. MTB strains resistance to INH is mainly caused by the alterations in several genes encoding the molecular targets as follows： catalase peroxidase （katG）,
