Objective:To observe the clinical effect of tuina plus Baixiao moxibustion in the treatment of temporomandibular joint dysfunction syndrome(TJDS).Methods:A total of 70 TJDS patients who met the inclusion criteria were...Objective:To observe the clinical effect of tuina plus Baixiao moxibustion in the treatment of temporomandibular joint dysfunction syndrome(TJDS).Methods:A total of 70 TJDS patients who met the inclusion criteria were randomized into an observation group and a control group by flipping a coin,with 35 cases in each group.Patients in the observation group were treated with tuina plus Baixiao moxibustion,while patients in the control group received oral intake of diclofenac potassium(75 mg/pill),1 pill after every dinner.Both tuina and Baixiao moxibustion were done once a day during treatment.The therapeutic evaluation was evaluated after 10 treatments in both groups.The maximum mouth opening distanee and visual analog scale(VAS)were observed before and after treatment,and the therapeutic efficacy was also compared.Results:After treatment,the maximum mouth opening distanee and VAS improved in both groups(all P<0.05);both items in the observation group were superior to those in the control group(both P<00.05).The total effective rate was 91.4%in the observation group,versus 74.3%in the control group,and the between-group comparison of the total effective rate showed statistical significanee(P<0.05).Conclusion:Tuina plus Baixiao moxibustion can effectively improve TJDS patient's temporomandibular joint function and alleviate pain,with better efficacy than oral intake of diclofenac potassium.展开更多
Objective： To study the effect of curcumin on fibroblasts in rats with cardiac fibrosis. Methods： The rats were randomly divided into 4 groups（n=12 in each group）： the normal control, isoproterenol（ISO）, ISO co...Objective： To study the effect of curcumin on fibroblasts in rats with cardiac fibrosis. Methods： The rats were randomly divided into 4 groups（n=12 in each group）： the normal control, isoproterenol（ISO）, ISO combined with low-dose curcumin（ISO＋Cur-L）, and ISO combined with high-dose curcumin（ISO＋Cur-H） groups. ISO＋Cur-L and ISO＋Cur-H groups were treated with curcumin（150 or 300 mg·kg-1·day-1） for 28 days. The primary culture of rat cardiac fibroblast was processed by trypsin digestion method in vitro. The 3rd to 5th generation were used for experiment. Western blot method was used to test the expression of collagen type Ⅰ/Ⅲ, α-smooth muscle actin（α-SMA）, transforming growth factor（TGF）-β1, matrix metalloproteinase（MMP）-9 and tissue inhibitor of metalloproteinase（TIMP）-1. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetr-azolium bromide（MTT） assay was applied to test the proliferation of fibroblast. Result： Curcumin significantly decreased interstitial and perivascular myocardial collagen deposition and cardiac weight index with reducing protein expression of collagen type Ⅰ/Ⅲ in hearts（P〈0.05）. In addition, curcumin directly inhibited angiotensin（Ang） Ⅱ-induced fibroblast proliferation and collagen type Ⅰ/Ⅲ expression in cardiac fibroblasts（P〈0.05）. Curcumin also inhibited fibrosis by inhibiting myofibroblast differentiation, decreased TGF-β1, MMP-9 and TIMP-1 expression（P〈0.05） but had no effects on Smad3 in Ang Ⅱ incubated cardiac fibroblasts. Conclusions： Curcumin reduces cardiac fibrosis in rats and Ang Ⅱ-induced fibroblast proliferation by inhibiting myofibroblast differentiation, decreasing collagen synthesis and accelerating collagen degradation through reduction of TGF-β1, MMPs/TIMPs. The present findings also provided novel insights into the role of curcumin as an anti-fibrotic agent for the treatment of cardiac fibrosis.展开更多
文摘Objective:To observe the clinical effect of tuina plus Baixiao moxibustion in the treatment of temporomandibular joint dysfunction syndrome(TJDS).Methods:A total of 70 TJDS patients who met the inclusion criteria were randomized into an observation group and a control group by flipping a coin,with 35 cases in each group.Patients in the observation group were treated with tuina plus Baixiao moxibustion,while patients in the control group received oral intake of diclofenac potassium(75 mg/pill),1 pill after every dinner.Both tuina and Baixiao moxibustion were done once a day during treatment.The therapeutic evaluation was evaluated after 10 treatments in both groups.The maximum mouth opening distanee and visual analog scale(VAS)were observed before and after treatment,and the therapeutic efficacy was also compared.Results:After treatment,the maximum mouth opening distanee and VAS improved in both groups(all P<0.05);both items in the observation group were superior to those in the control group(both P<00.05).The total effective rate was 91.4%in the observation group,versus 74.3%in the control group,and the between-group comparison of the total effective rate showed statistical significanee(P<0.05).Conclusion:Tuina plus Baixiao moxibustion can effectively improve TJDS patient's temporomandibular joint function and alleviate pain,with better efficacy than oral intake of diclofenac potassium.
基金Supported by the Guangdong Province Talents Project in Colleges and Universities(No.2050205)
文摘Objective： To study the effect of curcumin on fibroblasts in rats with cardiac fibrosis. Methods： The rats were randomly divided into 4 groups（n=12 in each group）： the normal control, isoproterenol（ISO）, ISO combined with low-dose curcumin（ISO＋Cur-L）, and ISO combined with high-dose curcumin（ISO＋Cur-H） groups. ISO＋Cur-L and ISO＋Cur-H groups were treated with curcumin（150 or 300 mg·kg-1·day-1） for 28 days. The primary culture of rat cardiac fibroblast was processed by trypsin digestion method in vitro. The 3rd to 5th generation were used for experiment. Western blot method was used to test the expression of collagen type Ⅰ/Ⅲ, α-smooth muscle actin（α-SMA）, transforming growth factor（TGF）-β1, matrix metalloproteinase（MMP）-9 and tissue inhibitor of metalloproteinase（TIMP）-1. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetr-azolium bromide（MTT） assay was applied to test the proliferation of fibroblast. Result： Curcumin significantly decreased interstitial and perivascular myocardial collagen deposition and cardiac weight index with reducing protein expression of collagen type Ⅰ/Ⅲ in hearts（P〈0.05）. In addition, curcumin directly inhibited angiotensin（Ang） Ⅱ-induced fibroblast proliferation and collagen type Ⅰ/Ⅲ expression in cardiac fibroblasts（P〈0.05）. Curcumin also inhibited fibrosis by inhibiting myofibroblast differentiation, decreased TGF-β1, MMP-9 and TIMP-1 expression（P〈0.05） but had no effects on Smad3 in Ang Ⅱ incubated cardiac fibroblasts. Conclusions： Curcumin reduces cardiac fibrosis in rats and Ang Ⅱ-induced fibroblast proliferation by inhibiting myofibroblast differentiation, decreasing collagen synthesis and accelerating collagen degradation through reduction of TGF-β1, MMPs/TIMPs. The present findings also provided novel insights into the role of curcumin as an anti-fibrotic agent for the treatment of cardiac fibrosis.
