OBJECTIVE: To investigate the effect of baijinpingchuan(白金平喘, BJPC) on the asthma rat model and identify differential metabolites and disturbed metabolic pathways. METHODS: The rats were categorized into six group...OBJECTIVE: To investigate the effect of baijinpingchuan(白金平喘, BJPC) on the asthma rat model and identify differential metabolites and disturbed metabolic pathways. METHODS: The rats were categorized into six groups: control, dexamethasone(DEX), ovalbumin(OVA), and low-, median-, and high-dose BJPC. The rats, except for the control group, were initially treated with OVA to develop the asthma model, which was then activated using DEX, OVA, and low-, median-, and high-dose BJPC. Enzyme-linked immunosorbent assay kit was used to detect the expression of interleukin(IL)-33, IL-25, thymic stromal lymphopoietin(TSLP), and transforming growth factor-beta 1(TGF-β1). Hematoxylin and eosin staining were performed to observe the pathological condition of the lung. Untargeted serum metabonomic analysis was conducted to identify differential metabolites and disturbed metabolic pathways. RESULTS: High-dose BJPC significantly inhibited the expression of IL-33, IL-25, TSLP, and TGF-β1(P < 0.0001). Further, high-dose BJPC improved inflammatory cell infiltration, which plays a similar role in asthma as DEX. OVA-induced and BJPC-treated rats were identified through 17 differential metabolites, especially cholic acid. Furthermore, primary bile acid biosynthesis was a significantly differential pathway in the mechanism of BJPC for treating asthma. CONCLUSIONS: BJPC plays an anti-inflammation role in asthma, which might be a promising therapy through mediating primary bile acid biosynthesis.展开更多
基金Science and Technology Development Plan Project of Jilin Provincial:Research on the Development of Baijin Pingchuan Granules as an In-hospital Preparation for the Treatment of Pediatric Asthma (Heat-type Asthma)(No. 20200404043YY)Traditional Chinese Medicine Science and Technology Project of Jilin Provincial:Exploring the Mechanism of Baijin Pingchuan Granules in Alleviating Airway Inflammation in Asthma based on the “Three-Stage Differentiation and Treatment” Theory of National Medical Masters (2020090)。
文摘OBJECTIVE: To investigate the effect of baijinpingchuan(白金平喘, BJPC) on the asthma rat model and identify differential metabolites and disturbed metabolic pathways. METHODS: The rats were categorized into six groups: control, dexamethasone(DEX), ovalbumin(OVA), and low-, median-, and high-dose BJPC. The rats, except for the control group, were initially treated with OVA to develop the asthma model, which was then activated using DEX, OVA, and low-, median-, and high-dose BJPC. Enzyme-linked immunosorbent assay kit was used to detect the expression of interleukin(IL)-33, IL-25, thymic stromal lymphopoietin(TSLP), and transforming growth factor-beta 1(TGF-β1). Hematoxylin and eosin staining were performed to observe the pathological condition of the lung. Untargeted serum metabonomic analysis was conducted to identify differential metabolites and disturbed metabolic pathways. RESULTS: High-dose BJPC significantly inhibited the expression of IL-33, IL-25, TSLP, and TGF-β1(P < 0.0001). Further, high-dose BJPC improved inflammatory cell infiltration, which plays a similar role in asthma as DEX. OVA-induced and BJPC-treated rats were identified through 17 differential metabolites, especially cholic acid. Furthermore, primary bile acid biosynthesis was a significantly differential pathway in the mechanism of BJPC for treating asthma. CONCLUSIONS: BJPC plays an anti-inflammation role in asthma, which might be a promising therapy through mediating primary bile acid biosynthesis.
