Liver fibrosis is the common consequence underlying most chronic liver diseases.Guizhifuling(GZFL)pills are a traditional medicine described in Jin Gui Yao Lue,which has been confirmed to exert an anti-fibrotic effect...Liver fibrosis is the common consequence underlying most chronic liver diseases.Guizhifuling(GZFL)pills are a traditional medicine described in Jin Gui Yao Lue,which has been confirmed to exert an anti-fibrotic effect.However,underlying mechanisms of GZFL in liver fibrosis remain understood.The present study was aimed to investigate the protective effects and the underlying mechanism of GZFL on liver fibrosis.We established the carbon tetrachloride(CCl,)-model of mice to induce hepatic fibrosis.展开更多
OBJECTIVE: To evaluate the protective effects and the underlying mechanism of Guizhifuling pill(桂枝茯苓丸, GZFL) on carbon tetrachloride(CCl4)-induced hepatic fibrosis in mice. METHODS: Male ICR mice by intraperitone...OBJECTIVE: To evaluate the protective effects and the underlying mechanism of Guizhifuling pill(桂枝茯苓丸, GZFL) on carbon tetrachloride(CCl4)-induced hepatic fibrosis in mice. METHODS: Male ICR mice by intraperitoneally administered with 20% CCl4(mixed 1∶4 in soybean oil) to induce liver fibrosis. Mice that underwent CCl4 were orally with GZFL. Using hematoxylin and eosin and Masson staining to examine the pathological changes in liver tissue. Serum biochemical parameters, antioxidant enzyme activity and proinflammatory cytokines was assessed. Nuclear factor-kappa B(NF-κB) pathway and nuclear factor-erythroid 2-related factor 2(Nrf2) family members were evaluated by Western blotting. RESULTS: Our findings indicated that GZFL could effectively suppress the progression of liver fibrosis in mice, which was determined based on the improvement in liver function and reduction of collagen deposition. GZFL treatment also decreased the level of cytokines and increased the activity of antioxidant enzymes in liver tissue. Moreover, GZFL exerted anti-inflammatory and antioxidant effects through regulating the Nrf2-mediated antioxidant system and inhibiting the NF-κB pathway. CONCLUSIONS: GZFL may prevent the progression of liver fibrosis by regulating the Nrf2/heme oxygenase-1 and NF-κB signaling pathways, thereby highlighting its role in the management of liver fibrosis.展开更多
文摘Liver fibrosis is the common consequence underlying most chronic liver diseases.Guizhifuling(GZFL)pills are a traditional medicine described in Jin Gui Yao Lue,which has been confirmed to exert an anti-fibrotic effect.However,underlying mechanisms of GZFL in liver fibrosis remain understood.The present study was aimed to investigate the protective effects and the underlying mechanism of GZFL on liver fibrosis.We established the carbon tetrachloride(CCl,)-model of mice to induce hepatic fibrosis.
基金Supported by the Zhejiang Provincial Key Research Project of Traditional Chinese Medicine Science and Technology Plan:Guizhi Fuling Pill Up-regulates MiR-29b-3p and Inhibits CUGBP1-mediated Study on the Mechanism of Liver Fibrosis (No. 2019ZZ017)。
文摘OBJECTIVE: To evaluate the protective effects and the underlying mechanism of Guizhifuling pill(桂枝茯苓丸, GZFL) on carbon tetrachloride(CCl4)-induced hepatic fibrosis in mice. METHODS: Male ICR mice by intraperitoneally administered with 20% CCl4(mixed 1∶4 in soybean oil) to induce liver fibrosis. Mice that underwent CCl4 were orally with GZFL. Using hematoxylin and eosin and Masson staining to examine the pathological changes in liver tissue. Serum biochemical parameters, antioxidant enzyme activity and proinflammatory cytokines was assessed. Nuclear factor-kappa B(NF-κB) pathway and nuclear factor-erythroid 2-related factor 2(Nrf2) family members were evaluated by Western blotting. RESULTS: Our findings indicated that GZFL could effectively suppress the progression of liver fibrosis in mice, which was determined based on the improvement in liver function and reduction of collagen deposition. GZFL treatment also decreased the level of cytokines and increased the activity of antioxidant enzymes in liver tissue. Moreover, GZFL exerted anti-inflammatory and antioxidant effects through regulating the Nrf2-mediated antioxidant system and inhibiting the NF-κB pathway. CONCLUSIONS: GZFL may prevent the progression of liver fibrosis by regulating the Nrf2/heme oxygenase-1 and NF-κB signaling pathways, thereby highlighting its role in the management of liver fibrosis.
