An offshore shoal or bar refracts ocean surface waves and causes wave focusing/defocusing on the adjacent beach. Wave focal patterns characterized by alongshore variations in wave height, wave angle, and breaking loca...An offshore shoal or bar refracts ocean surface waves and causes wave focusing/defocusing on the adjacent beach. Wave focal patterns characterized by alongshore variations in wave height, wave angle, and breaking location induce alongshore non-uniformities of wave setup and nearshore circulation, e.g., rip currents and alongshore currents, in the surfzone. A simplified analytic model for nearshore circulation generated by focused/defocused waves on a planar beach is developed and theoretical solutions are obtained using transport stream function and perturbations in alongshore distributions of wave height and wave angle at the breaker line. The analytic model suggests that alongshore currents are strongly affected by a pair of counter-rotating vortices generated shoreward of the wave focal zone. The vortices are persistent, and their strengths depend on the amplitudes of alongshore variations in wave height and wave angle. The alongshore gradient in wave height tends to intensify the vortices while the convergence of wave angle tends to weaken the vortices. Divergent flows associated with the vortices in the surfzone are intense, strengthening alongshore currents in the downstream of the wave focal zone and weakening alongshore currents or causing flows reversal in the upstream. Alongshore currents are modulated by rip currents associated with the wave focusing/defocusing patterns.展开更多
The tetracycline （Tet）-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and re...The tetracycline （Tet）-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and repeatedly replenishing tetracycline to turn off the constitutively active system is a tedious process. To solve these problems, a Tet-off and heat shock （HS）-on gene expression regulation system was constructed in this study. This system is composed of a chimeric transactivator gene TetR-HSF that is derived from a Tet repressor （TetR） and a HS transcription factor （HSF） controlled by a HS promoter HSP70m, and a Tet operator containing hybrid promoter, Om35S, that drives expression of the β-glucuronidase （GUS） gene. The resultant system yields a GUS expression pattern similar to that of the HSP70m promoter under inducing temperatures and at 35 and 40℃ drives GUS expression to a similar level as the Cauliflower mosaic virus （CaMV） 35S promoter. Further examination revealed that the TetR-HSF and GUS genes were induced by HS, reaching peak expression after 1 and 6 h treatment, respectively, and the HS induction of the expression system could be inhibited by Tet. This system will provide a useful tool for transgenic studies of plants in the laboratory and in the field, including transgene function analysis, agronomic trait improvement, biopharmaceutical protein production and others.展开更多
基金financially supported by the Tianjin Municipal Science and Technology Commission(Grant No.19JCYBJC22000)
文摘An offshore shoal or bar refracts ocean surface waves and causes wave focusing/defocusing on the adjacent beach. Wave focal patterns characterized by alongshore variations in wave height, wave angle, and breaking location induce alongshore non-uniformities of wave setup and nearshore circulation, e.g., rip currents and alongshore currents, in the surfzone. A simplified analytic model for nearshore circulation generated by focused/defocused waves on a planar beach is developed and theoretical solutions are obtained using transport stream function and perturbations in alongshore distributions of wave height and wave angle at the breaker line. The analytic model suggests that alongshore currents are strongly affected by a pair of counter-rotating vortices generated shoreward of the wave focal zone. The vortices are persistent, and their strengths depend on the amplitudes of alongshore variations in wave height and wave angle. The alongshore gradient in wave height tends to intensify the vortices while the convergence of wave angle tends to weaken the vortices. Divergent flows associated with the vortices in the surfzone are intense, strengthening alongshore currents in the downstream of the wave focal zone and weakening alongshore currents or causing flows reversal in the upstream. Alongshore currents are modulated by rip currents associated with the wave focusing/defocusing patterns.
基金supported by the National High-Tech R&D Program of China(2010AA10060705)the Transgenic Engineering Crops Breeding Special Funds from China’s Ministry of Agriculture(2009ZX08010-005B)
文摘The tetracycline （Tet）-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and repeatedly replenishing tetracycline to turn off the constitutively active system is a tedious process. To solve these problems, a Tet-off and heat shock （HS）-on gene expression regulation system was constructed in this study. This system is composed of a chimeric transactivator gene TetR-HSF that is derived from a Tet repressor （TetR） and a HS transcription factor （HSF） controlled by a HS promoter HSP70m, and a Tet operator containing hybrid promoter, Om35S, that drives expression of the β-glucuronidase （GUS） gene. The resultant system yields a GUS expression pattern similar to that of the HSP70m promoter under inducing temperatures and at 35 and 40℃ drives GUS expression to a similar level as the Cauliflower mosaic virus （CaMV） 35S promoter. Further examination revealed that the TetR-HSF and GUS genes were induced by HS, reaching peak expression after 1 and 6 h treatment, respectively, and the HS induction of the expression system could be inhibited by Tet. This system will provide a useful tool for transgenic studies of plants in the laboratory and in the field, including transgene function analysis, agronomic trait improvement, biopharmaceutical protein production and others.
