Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility(AID) and standardized ileal digestibility(SID) of amino acids(AA) in...Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility(AID) and standardized ileal digestibility(SID) of amino acids(AA) in growing pigs. A total of 24 ileal-cannulated pigs(Duroc×(Large White×Landrace) with body weight(21.4±1.5) kg) were randomly allotted to 4 treatments. The pigs were provided a corn-soybean meal diet or a diet containing 5, 10 or 20% of alfalfa meal during two 10-d experimental periods. The AID of AA was measured. Six ileal-cannulated pigs were fed a protein-free diet in order to estimate the endogenous protein losses and SID of AA. Ileal AA digestibility was not affected by inclusion of 5 or 10% alfalfa meal in the diet(P〉0.05). The AID of His, Lys, Met, Phe, Thr, Val, Ala, Asp, Cys, Gly, Pro, Ser and Tyr reduced by 2.0–6.8% with the addition level of alfalfa meal(linear, P〈0.05). The SID of His, Lys, Ser, Thr and Tyr decreased by 2.2–4.3% as the level of alfalfa meal in the diet increased(linear, P〈0.05). The AID and SID of AA were not affected by the sampling time(P〉0.05). A multiple linear regression analysis, taking into account both the soluble and insoluble fiber content in the diets, explained more than 36% of variation in SID of Ser and Thr(P〈0.05). In conclusion, the corn-soybean meal diet containing 10% of alfalfa meal did not affect ileal AA digestion. The AID and SID of AA were similar between two sampling times. Increasing the concentration of total dietary fiber from 12.3 to 21.4% by adding graded levels of alfalfa meal(0–20%) to a corn-soybean meal control diet induced a linear reduction in AID and SID of most AA. Soluble and insoluble fibers from alfalfa meal have differential roles in the AA digestion, which may help explain the variation observed in the SID of partial AA. These findings would provide important information for dietary fiber level and composition related to AA digestion.展开更多
基金financially supported by the Project of State Key Laboratory of Animal Nutrition,Ministry of Science and Technology,China(2004DA125184G1104)the Agricultural Science and Technology Innovation Program,Chinese Academy of Agricultural Sciences,China(ASTIP-IAS07)
文摘Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility(AID) and standardized ileal digestibility(SID) of amino acids(AA) in growing pigs. A total of 24 ileal-cannulated pigs(Duroc×(Large White×Landrace) with body weight(21.4±1.5) kg) were randomly allotted to 4 treatments. The pigs were provided a corn-soybean meal diet or a diet containing 5, 10 or 20% of alfalfa meal during two 10-d experimental periods. The AID of AA was measured. Six ileal-cannulated pigs were fed a protein-free diet in order to estimate the endogenous protein losses and SID of AA. Ileal AA digestibility was not affected by inclusion of 5 or 10% alfalfa meal in the diet(P〉0.05). The AID of His, Lys, Met, Phe, Thr, Val, Ala, Asp, Cys, Gly, Pro, Ser and Tyr reduced by 2.0–6.8% with the addition level of alfalfa meal(linear, P〈0.05). The SID of His, Lys, Ser, Thr and Tyr decreased by 2.2–4.3% as the level of alfalfa meal in the diet increased(linear, P〈0.05). The AID and SID of AA were not affected by the sampling time(P〉0.05). A multiple linear regression analysis, taking into account both the soluble and insoluble fiber content in the diets, explained more than 36% of variation in SID of Ser and Thr(P〈0.05). In conclusion, the corn-soybean meal diet containing 10% of alfalfa meal did not affect ileal AA digestion. The AID and SID of AA were similar between two sampling times. Increasing the concentration of total dietary fiber from 12.3 to 21.4% by adding graded levels of alfalfa meal(0–20%) to a corn-soybean meal control diet induced a linear reduction in AID and SID of most AA. Soluble and insoluble fibers from alfalfa meal have differential roles in the AA digestion, which may help explain the variation observed in the SID of partial AA. These findings would provide important information for dietary fiber level and composition related to AA digestion.