黑色素合成受多种基因和miRNAs的调控,为了研究miR-186-5p与α-MSH协同作用对绵羊黑素细胞黑色素生成的影响。本研究在绵羊黑素细胞中转染miR-186-5p表达载体,同时添加α-MSH,通过实时荧光PCR (quantitative real time polymerase chain...黑色素合成受多种基因和miRNAs的调控,为了研究miR-186-5p与α-MSH协同作用对绵羊黑素细胞黑色素生成的影响。本研究在绵羊黑素细胞中转染miR-186-5p表达载体,同时添加α-MSH,通过实时荧光PCR (quantitative real time polymerase chain reaction, qRT-PCR)和蛋白质免疫印迹(Western blotting)检测MITF、TYR、TYRP1和TYRP2 4种可能与之相关的基因表达情况;利用免疫组化检测MITF蛋白在黑素细胞中的表达和亚细胞定位;通过分光光度法测定黑色素含量变化;利用划痕试验测定细胞迁移情况,试验分为miR-186-5p转染组、miR-186-5p+α-MSH互作组和对照组,每组3个重复。结果显示,miR-186-5p能够抑制绵羊黑素细胞中MITF、TYR、TYRP1和TYRP2的表达,并最终抑制黑色素的生成。而添加α-MSH能缓解miR-186-5p对MITF、TYR、TYRP1和TYRP2表达的抑制作用,进而在一定程度上恢复黑色素的含量。另外,miR-186-5p还能抑制细胞分裂,并阻碍细胞的迁移,而α-MSH无法抵消miR-186-5p产生的这种负面影响。上述结果表明,在绵羊黑素细胞中,miR-186-5p和α-MSH均参与调控黑色素合成途径,其中,miR-186-5p主要起负调控作用,而α-MSH主要参与相关位点的正调控,并能部分恢复miR-186-5p导致的黑色素含量下降。值得注意的是,miR-186-5p还参与调控细胞的增殖和迁移,而α-MSH不参与相关调控。展开更多
Due to the attractive performances such as the ability of beam focus,broadband,multi-beam scanning and other features,Luneburg lens antennas are applied in multi-beam antenna,which overcomes the problem of gain loss p...Due to the attractive performances such as the ability of beam focus,broadband,multi-beam scanning and other features,Luneburg lens antennas are applied in multi-beam antenna,which overcomes the problem of gain loss produced by multi-beam parabolic antenna.Based on 3-D printing technique,Luneburg lens antennas by drilling holes are studied.Permittivity and loss tangent of the equivalent lens materials can be influenced by original materials,hole shapes,hole directions,and porosity.After tests,polystyrene with waxes may be the most appropriate materials for Luneburg lens with high strength.Permittivity with the shape of triangle is the lowest due to the homogeneity.Relative permittivities with the direction at a range of 15°-45°are lower while loss tangent at a range of 0°-30°.Radial directional holes are more appropriate for Luneburg lens.The relative permittivity is decreased with the increment of porosity.After calculations,the forecasts calculated by Looyenga and A-BG theory are more precise.Finally,Luneburg lens with two layers is fabricated by 3-D printing.展开更多
文摘黑色素合成受多种基因和miRNAs的调控,为了研究miR-186-5p与α-MSH协同作用对绵羊黑素细胞黑色素生成的影响。本研究在绵羊黑素细胞中转染miR-186-5p表达载体,同时添加α-MSH,通过实时荧光PCR (quantitative real time polymerase chain reaction, qRT-PCR)和蛋白质免疫印迹(Western blotting)检测MITF、TYR、TYRP1和TYRP2 4种可能与之相关的基因表达情况;利用免疫组化检测MITF蛋白在黑素细胞中的表达和亚细胞定位;通过分光光度法测定黑色素含量变化;利用划痕试验测定细胞迁移情况,试验分为miR-186-5p转染组、miR-186-5p+α-MSH互作组和对照组,每组3个重复。结果显示,miR-186-5p能够抑制绵羊黑素细胞中MITF、TYR、TYRP1和TYRP2的表达,并最终抑制黑色素的生成。而添加α-MSH能缓解miR-186-5p对MITF、TYR、TYRP1和TYRP2表达的抑制作用,进而在一定程度上恢复黑色素的含量。另外,miR-186-5p还能抑制细胞分裂,并阻碍细胞的迁移,而α-MSH无法抵消miR-186-5p产生的这种负面影响。上述结果表明,在绵羊黑素细胞中,miR-186-5p和α-MSH均参与调控黑色素合成途径,其中,miR-186-5p主要起负调控作用,而α-MSH主要参与相关位点的正调控,并能部分恢复miR-186-5p导致的黑色素含量下降。值得注意的是,miR-186-5p还参与调控细胞的增殖和迁移,而α-MSH不参与相关调控。
基金supported by the Science and Technology Programme of Shijiazhuang under Grant 151130081A
文摘Due to the attractive performances such as the ability of beam focus,broadband,multi-beam scanning and other features,Luneburg lens antennas are applied in multi-beam antenna,which overcomes the problem of gain loss produced by multi-beam parabolic antenna.Based on 3-D printing technique,Luneburg lens antennas by drilling holes are studied.Permittivity and loss tangent of the equivalent lens materials can be influenced by original materials,hole shapes,hole directions,and porosity.After tests,polystyrene with waxes may be the most appropriate materials for Luneburg lens with high strength.Permittivity with the shape of triangle is the lowest due to the homogeneity.Relative permittivities with the direction at a range of 15°-45°are lower while loss tangent at a range of 0°-30°.Radial directional holes are more appropriate for Luneburg lens.The relative permittivity is decreased with the increment of porosity.After calculations,the forecasts calculated by Looyenga and A-BG theory are more precise.Finally,Luneburg lens with two layers is fabricated by 3-D printing.
