Selenium(Se)is an essential trace element that has a large number of biological functions for broilers.However,the absorption kinetics of Se from sodium selenite in the small intestine of broilers remains unclear.Ther...Selenium(Se)is an essential trace element that has a large number of biological functions for broilers.However,the absorption kinetics of Se from sodium selenite in the small intestine of broilers remains unclear.Therefore,two experiments were conducted with 28-d-old commercial male broilers to study the kinetics of Se absorption in ligated small intestinal segments.In experiment 1,the Se absorption in duodenal,jejunal,and ileal segments at different post-perfusion time points(0,20,40,60,80,100 and 120 min)were compared.In experiment 2,a kinetic study of Se absorption was conducted with the duodenal,jejunal,and ileal loops perfused with solutions containing 0,0.0375,0.075,0.15,0.30,or 0.60μg mL^-1 of Se as sodium selenite,and Se contents in perfusates were determined at 100 min after perfusion.The results from experiment 1 showed that the Se absorption increased in an asymptotic response(P<0.0001)to post-perfusion time within 120 min in all the small intestinal segments,but increased linearly(P<0.0001)at less than 100 min after perfusion in duodenal and ileal segments,while more than 96.0%of the maximum Se absorption occurred at 100 min after perfusion in each small intestinal segment.In experiment 2,there was no difference(P>0.05)in the Se absorption rate among different ligated small intestinal segments perfused with solutions containing 0.0375–0.15μg mL^-1 of Se,however,the Se absorption rate was higher(P<0.05)in the jejunum than that in the duodenum perfused with solutions containing 0.30–0.60μg mL^-1 of Se.The kinetic curves of Se absorption demonstrated that the Se absorption was a saturated carrier-mediated process in the duodenum,and the maximum absorption rate was 1271 pg min^-1 cm^-1;whereas the Se absorptions were a non-saturated diffusion process in the jejunum and ileum,and the diffusive constants were 2107 and 1777 cm2 min^-1,respectively.The results from the present study indicate that the jejunum is the main Se absorption site,and the Se absorption is a saturated carrier-mediated process in the duodenum,but a non-saturated diffusion process in the jejunum and ileum of broilers.展开更多
目的:系统评价和Meta分析舒洛地特(sulodexide,SDX)联合辛伐他汀(simvastatin)用于糖尿病下肢血管病变治疗有效性和安全性。方法:计算机检索中国期刊全文数据库、万方数据库、维普数据库、SinoMed数据库、PubMed数据库、Cochrane Librar...目的:系统评价和Meta分析舒洛地特(sulodexide,SDX)联合辛伐他汀(simvastatin)用于糖尿病下肢血管病变治疗有效性和安全性。方法:计算机检索中国期刊全文数据库、万方数据库、维普数据库、SinoMed数据库、PubMed数据库、Cochrane Library数据库、EMbase数据库、Web of Science数据库,搜集舒洛地特联合辛伐他汀治疗糖尿病下肢血管病变的有效性和安全性的随机对照研究(RCT),检索时间均从建库至2019年8月31日。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan5.3软件进行Meta分析。结果:共纳入9篇RCTs,包括781例患者。Meta分析结果显示:与单纯使用舒洛地特组相比,舒洛地特联合辛伐他汀治疗组能提高行走无痛距离、最长行走距离、血流速度(Vm)、搏动指数(PI),降低血流阻力指数(RI)及不良反应发生率。结论:当前纳入文献表明,舒洛地特联合辛伐他汀对糖尿病下肢血管病变治疗的有效性和安全性较高。受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。展开更多
基金financially supported by the National Natural Science Foundation of China(31601956)the Agricultural Science and Technology Innovation Program,China(ASTIP-IAS09)the earmarked fund for China Agriculture Research System(CARS-41)。
文摘Selenium(Se)is an essential trace element that has a large number of biological functions for broilers.However,the absorption kinetics of Se from sodium selenite in the small intestine of broilers remains unclear.Therefore,two experiments were conducted with 28-d-old commercial male broilers to study the kinetics of Se absorption in ligated small intestinal segments.In experiment 1,the Se absorption in duodenal,jejunal,and ileal segments at different post-perfusion time points(0,20,40,60,80,100 and 120 min)were compared.In experiment 2,a kinetic study of Se absorption was conducted with the duodenal,jejunal,and ileal loops perfused with solutions containing 0,0.0375,0.075,0.15,0.30,or 0.60μg mL^-1 of Se as sodium selenite,and Se contents in perfusates were determined at 100 min after perfusion.The results from experiment 1 showed that the Se absorption increased in an asymptotic response(P<0.0001)to post-perfusion time within 120 min in all the small intestinal segments,but increased linearly(P<0.0001)at less than 100 min after perfusion in duodenal and ileal segments,while more than 96.0%of the maximum Se absorption occurred at 100 min after perfusion in each small intestinal segment.In experiment 2,there was no difference(P>0.05)in the Se absorption rate among different ligated small intestinal segments perfused with solutions containing 0.0375–0.15μg mL^-1 of Se,however,the Se absorption rate was higher(P<0.05)in the jejunum than that in the duodenum perfused with solutions containing 0.30–0.60μg mL^-1 of Se.The kinetic curves of Se absorption demonstrated that the Se absorption was a saturated carrier-mediated process in the duodenum,and the maximum absorption rate was 1271 pg min^-1 cm^-1;whereas the Se absorptions were a non-saturated diffusion process in the jejunum and ileum,and the diffusive constants were 2107 and 1777 cm2 min^-1,respectively.The results from the present study indicate that the jejunum is the main Se absorption site,and the Se absorption is a saturated carrier-mediated process in the duodenum,but a non-saturated diffusion process in the jejunum and ileum of broilers.
文摘目的:系统评价和Meta分析舒洛地特(sulodexide,SDX)联合辛伐他汀(simvastatin)用于糖尿病下肢血管病变治疗有效性和安全性。方法:计算机检索中国期刊全文数据库、万方数据库、维普数据库、SinoMed数据库、PubMed数据库、Cochrane Library数据库、EMbase数据库、Web of Science数据库,搜集舒洛地特联合辛伐他汀治疗糖尿病下肢血管病变的有效性和安全性的随机对照研究(RCT),检索时间均从建库至2019年8月31日。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan5.3软件进行Meta分析。结果:共纳入9篇RCTs,包括781例患者。Meta分析结果显示:与单纯使用舒洛地特组相比,舒洛地特联合辛伐他汀治疗组能提高行走无痛距离、最长行走距离、血流速度(Vm)、搏动指数(PI),降低血流阻力指数(RI)及不良反应发生率。结论:当前纳入文献表明,舒洛地特联合辛伐他汀对糖尿病下肢血管病变治疗的有效性和安全性较高。受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。
