Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was...Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was used as reduction agent of antibody and the reduction of 186 ReO4^- was finished in sodium gluconate solution(pH=2.54),the labeling efficiency was 96.2%,After purification,the labeled products were tested in respect of immunoreactivity,in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice,188ReO4^- labeled colloid was synthesized by using a two-step method.The size of radiocolloid particles was around 2-5μm,and the radiochemical yield was over 95%,Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188Re-collied and ^188 Re-McAb-SZ39 was 90% and 65% ,respectively.展开更多
Human peripheral blood exposed with 188ReO4 at various radioactivities for 54h was examined to observe the chromosome and chromatin aberrations at metaphases in the mitosis of lymphocytes. The findings indicate that t...Human peripheral blood exposed with 188ReO4 at various radioactivities for 54h was examined to observe the chromosome and chromatin aberrations at metaphases in the mitosis of lymphocytes. The findings indicate that there is an increasing tendency of the aberration yields of both types with increasing 188ReO4 concentration and a dose dependency could be obtained. The aberration yield was best fitted by a quadratic model. Chromatin aberrations, aberration cells and chromosome fragments were fitted into a linear-regression model.展开更多
文摘Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was used as reduction agent of antibody and the reduction of 186 ReO4^- was finished in sodium gluconate solution(pH=2.54),the labeling efficiency was 96.2%,After purification,the labeled products were tested in respect of immunoreactivity,in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice,188ReO4^- labeled colloid was synthesized by using a two-step method.The size of radiocolloid particles was around 2-5μm,and the radiochemical yield was over 95%,Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188Re-collied and ^188 Re-McAb-SZ39 was 90% and 65% ,respectively.
文摘Human peripheral blood exposed with 188ReO4 at various radioactivities for 54h was examined to observe the chromosome and chromatin aberrations at metaphases in the mitosis of lymphocytes. The findings indicate that there is an increasing tendency of the aberration yields of both types with increasing 188ReO4 concentration and a dose dependency could be obtained. The aberration yield was best fitted by a quadratic model. Chromatin aberrations, aberration cells and chromosome fragments were fitted into a linear-regression model.
