目的通过建立体外心衰细胞模型,探讨人参附子不同配伍比例水煎液在相同浓度下对体外心衰细胞保护作用的强弱,筛选出疗效较好的人参附子配伍比例。方法取出生1~3 d SD大鼠的乳鼠心肌细胞进行原代培养用于实验;使用0.8%戊巴比妥钠加入心...目的通过建立体外心衰细胞模型,探讨人参附子不同配伍比例水煎液在相同浓度下对体外心衰细胞保护作用的强弱,筛选出疗效较好的人参附子配伍比例。方法取出生1~3 d SD大鼠的乳鼠心肌细胞进行原代培养用于实验;使用0.8%戊巴比妥钠加入心肌细胞培养板中得到心衰细胞模型;加入不同配伍比例人参附子水煎液,测得药物对心衰模型细胞离子转运相关酶活力的影响,测定培养液中脑钠肽(BNP)与肿瘤坏死因子(TNF-α)含量变化。结论2:1(人参:附子)组,1.5:1(人参:附子)组及1:1(人参:附子)组疗效较好,能够显著降低由心衰引起的TNF-α及BNP水平改变,通过调节心室肌细胞膜上离子转运相关酶活力,使心肌的收缩性加强,发挥抗心衰作用。展开更多
Recent studies have revealed that the property of drug is mainly associated with the body's substance and energy metabolism. The present study aimed to evaluate the drug property of Poria, called Fuling(FL) in tra...Recent studies have revealed that the property of drug is mainly associated with the body's substance and energy metabolism. The present study aimed to evaluate the drug property of Poria, called Fuling(FL) in traditional Chinese medicine(TCM), in terms of its effects on the substance and energy metabolism in rat models of cold-deficiency and heat-deficiency syndromes, compared with Aconiti Lateralis Radix Praeparaia, called Fuzi(FZ) in TCM, with hot property, and Anemarrhenae Rhizoma, called Zhimu(ZM) in TCM, with cold property, as reference drugs, respectively. The appearance score, toe and rectal temperatures of the animals treated were assessed at different time points. Several indices in vivo correlated with substance and energy metabolism(glucokinas, phosphoglycerate kinase, cytochrome c reductase, cytochrome c oxydase, and Na^+-K^+-ATPase), endocrine system(triiodothyronine, thyroxine, and 17-hydroxycorticosteroid), nervous system(acetylcholin esterase), and cyclic nucleotide system were determined. The changes in appearance score and indices in vivo suggested the successful establishment of cold-deficiency and heat-deficiency syndrome models. FZ reversed the decreased levels of indices(substance and energy metabolism and endocrine system) and alleviated the syndrome of cold-deficiency model, and ZM showed obviously therapeutic effect on heat-deficiency syndrome(appearance score, substance and energy metabolism, and endocrine system). FL could alleviate cold-deficiency syndrome and raise the decreased levels of glucokinas, phosphoglycerate kinase, cytochrome c reductase and triiodothyronine in cold-deficiency model, but had no significant effect on heat-deficiency syndrome. Drug property of FL was inferred as trending to "flat and warm", which still need further study. It was advisable to adopt both cold-deficiency and heat-deficiency models to study the drugs with "flat" property.展开更多
文摘目的通过建立体外心衰细胞模型,探讨人参附子不同配伍比例水煎液在相同浓度下对体外心衰细胞保护作用的强弱,筛选出疗效较好的人参附子配伍比例。方法取出生1~3 d SD大鼠的乳鼠心肌细胞进行原代培养用于实验;使用0.8%戊巴比妥钠加入心肌细胞培养板中得到心衰细胞模型;加入不同配伍比例人参附子水煎液,测得药物对心衰模型细胞离子转运相关酶活力的影响,测定培养液中脑钠肽(BNP)与肿瘤坏死因子(TNF-α)含量变化。结论2:1(人参:附子)组,1.5:1(人参:附子)组及1:1(人参:附子)组疗效较好,能够显著降低由心衰引起的TNF-α及BNP水平改变,通过调节心室肌细胞膜上离子转运相关酶活力,使心肌的收缩性加强,发挥抗心衰作用。
基金supported by National Basic Research Program of China(No.2013CB531803)
文摘Recent studies have revealed that the property of drug is mainly associated with the body's substance and energy metabolism. The present study aimed to evaluate the drug property of Poria, called Fuling(FL) in traditional Chinese medicine(TCM), in terms of its effects on the substance and energy metabolism in rat models of cold-deficiency and heat-deficiency syndromes, compared with Aconiti Lateralis Radix Praeparaia, called Fuzi(FZ) in TCM, with hot property, and Anemarrhenae Rhizoma, called Zhimu(ZM) in TCM, with cold property, as reference drugs, respectively. The appearance score, toe and rectal temperatures of the animals treated were assessed at different time points. Several indices in vivo correlated with substance and energy metabolism(glucokinas, phosphoglycerate kinase, cytochrome c reductase, cytochrome c oxydase, and Na^+-K^+-ATPase), endocrine system(triiodothyronine, thyroxine, and 17-hydroxycorticosteroid), nervous system(acetylcholin esterase), and cyclic nucleotide system were determined. The changes in appearance score and indices in vivo suggested the successful establishment of cold-deficiency and heat-deficiency syndrome models. FZ reversed the decreased levels of indices(substance and energy metabolism and endocrine system) and alleviated the syndrome of cold-deficiency model, and ZM showed obviously therapeutic effect on heat-deficiency syndrome(appearance score, substance and energy metabolism, and endocrine system). FL could alleviate cold-deficiency syndrome and raise the decreased levels of glucokinas, phosphoglycerate kinase, cytochrome c reductase and triiodothyronine in cold-deficiency model, but had no significant effect on heat-deficiency syndrome. Drug property of FL was inferred as trending to "flat and warm", which still need further study. It was advisable to adopt both cold-deficiency and heat-deficiency models to study the drugs with "flat" property.
