The growth performance,nutrient removal,lipid accumulation and morphological changes of Cyanobacterium aponinum OUC1 and Scenedesmus obliquus which were cultured in secondary effluents from two wastewater treatment pl...The growth performance,nutrient removal,lipid accumulation and morphological changes of Cyanobacterium aponinum OUC1 and Scenedesmus obliquus which were cultured in secondary effluents from two wastewater treatment plants:Tuandao Wastewater Treatment Plant(ETD)and Licun River Wastewater Treatment Plant(ELR)were investigated.The results showed that both C.aponinum OUC1 and S.obliquus have superior growth performances in both undiluted effluents,while the better of them was that in ETD effluent,with cell densities of C.aponinum OUC1 and S.obliquus increased by 159%and 66%over that of BG11(control),respectively.Regarding nutrient removal,S.obliquus could completely remove inorganic phosphorus,and decrease ammonia nitrogen in ETD effluent by 81%.In addition,both C.aponinum OUC1 and S.obliquus cultivated in ETD exhibited extraordinary potential for biofuel production,increasing lipid productivities by 133%and 89%of that cultivated in ELR,respectively.As to ultrastructural changes,the differences in the lipoidal globules and glycogen granules of S.obliquus and C.aponinum OUC1 among the ETD and ELR treatments were mostly related to phosphorus limitations.The findings from this research reveal the probability using the secondary effluents as cultivation media to enhance algal biomass,nutrient removal and lipid productivity.展开更多
1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on mar...1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.展开更多
基金This work was supported by the National Marine Hazard Mitigation Service,Ministry of Natural Resources of the People’s Republic of China through its Commissioned Research Scheme(No.2019005AC).
文摘The growth performance,nutrient removal,lipid accumulation and morphological changes of Cyanobacterium aponinum OUC1 and Scenedesmus obliquus which were cultured in secondary effluents from two wastewater treatment plants:Tuandao Wastewater Treatment Plant(ETD)and Licun River Wastewater Treatment Plant(ELR)were investigated.The results showed that both C.aponinum OUC1 and S.obliquus have superior growth performances in both undiluted effluents,while the better of them was that in ETD effluent,with cell densities of C.aponinum OUC1 and S.obliquus increased by 159%and 66%over that of BG11(control),respectively.Regarding nutrient removal,S.obliquus could completely remove inorganic phosphorus,and decrease ammonia nitrogen in ETD effluent by 81%.In addition,both C.aponinum OUC1 and S.obliquus cultivated in ETD exhibited extraordinary potential for biofuel production,increasing lipid productivities by 133%and 89%of that cultivated in ELR,respectively.As to ultrastructural changes,the differences in the lipoidal globules and glycogen granules of S.obliquus and C.aponinum OUC1 among the ETD and ELR treatments were mostly related to phosphorus limitations.The findings from this research reveal the probability using the secondary effluents as cultivation media to enhance algal biomass,nutrient removal and lipid productivity.
基金Supported by the National Marine Hazard Mitigation Service,China through its Commissioned Research Scheme(No.2017AB030)
文摘1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.
