This study aimed at gaining the cultivated land quality and the situation of soil pollution, taking Nehe City in Heilongjiang Province as an example, used ordinary Kriging interpolation of geostatistics, investigated ...This study aimed at gaining the cultivated land quality and the situation of soil pollution, taking Nehe City in Heilongjiang Province as an example, used ordinary Kriging interpolation of geostatistics, investigated the content and spatial distribution of eight kinds of heavy metal elements（namely Cd, Cr, Pb, Cu, Zn, Ni, Hg and As） in farming land soil, then analyzed the distinction and potential source of these heavy metal elements. The result showed that（1） all these elements had a certain amount of accumulation compared with those in the background of Heilongjiang Province, among which the accumulated levels of Cu and As were the highest.（2） Through statistic analyses, it showed that the spatial autocorrelation degrees of these four elements（namely Cd, Cr, Cu and Zn） were higher.（3） The pollution of Cd and Cu was relatively severe.（4） The spatial distribution features of these eight kinds of heavy metal elements were different, and the soil parent material and the human activity played double roles in the content distribution of heavy metal elements in farming land soil.展开更多
Alcoholic liver disease(ALD) has become one of the leading causes of death in the world. Berbamine(BM), a natural product mainly derived from Berberis vulgaris L, possesses multiple bioactivities as a traditional medi...Alcoholic liver disease(ALD) has become one of the leading causes of death in the world. Berbamine(BM), a natural product mainly derived from Berberis vulgaris L, possesses multiple bioactivities as a traditional medicine. However, the protective effect of BM on ALD remains unknown. In this study, we investigated the effect of BM on ethanol-induced hepatic injury in mice and its underlying mechanism. It was shown that BM at 0.3125-40 μmol·L^-1 had no effect on macrophages and hepatocytes proliferation. BM at 5-20 μmol·L^-1 significantly inhibited lipopolysaccharide(LPS) or acetate-induced IL-1β and IL-6 m RNA expression in RAW264.7 cells. Moreover, BM treatment significantly inhibited LPS-induced p65 and STAT3 phosphorylation in RAW264.7 cells. Hepatic histopathology analysis showed that inflammatory cells infiltration and lipid accumulation were suppressed by 25 and 50 mg·kg^-1 BM administration in ethanol-induced hepatic injury mouse model. Meanwhile, BM treatment significantly inhibited serum ALT and AST levels in ethanol-fed mice. Oil red O staining results showed that BM administration ameliorated hepatic lipid accumulation in ethanolfed mice. Preventions of ethanol-induced hepatic injury by BM were reflected by markedly decreased serum and hepatic triglyceride(TG) and total cholesterol(TC) contents. Real-time PCR results showed that BM treatment significantly inhibited pro-inflammatory cytokines m RNA expression in ethanol-fed mouse liver. Remarkably, the mechanism of action of BM was related to the reduction of ethanol-induced NF-κB and STAT3 phosphorylation levels in liver. In addition, BM treatment significantly inhibited ERK phosphorylation but not JNK and p38 of MAPK pathway. Taken together, our results demonstrate a beneficial effect of BM on ethanol-induced liver injury via a mechanism associated with inactivation of NF-κB, STAT3 and ERK pathway, which gives insight into the further evaluation of the therapeutic potential of BM for ALD.展开更多
基金Supported by the National Natural Science Foundation of China(D201401)the Natural Science Foundation of Heilongjiang Province of China
文摘This study aimed at gaining the cultivated land quality and the situation of soil pollution, taking Nehe City in Heilongjiang Province as an example, used ordinary Kriging interpolation of geostatistics, investigated the content and spatial distribution of eight kinds of heavy metal elements（namely Cd, Cr, Pb, Cu, Zn, Ni, Hg and As） in farming land soil, then analyzed the distinction and potential source of these heavy metal elements. The result showed that（1） all these elements had a certain amount of accumulation compared with those in the background of Heilongjiang Province, among which the accumulated levels of Cu and As were the highest.（2） Through statistic analyses, it showed that the spatial autocorrelation degrees of these four elements（namely Cd, Cr, Cu and Zn） were higher.（3） The pollution of Cd and Cu was relatively severe.（4） The spatial distribution features of these eight kinds of heavy metal elements were different, and the soil parent material and the human activity played double roles in the content distribution of heavy metal elements in farming land soil.
基金National Natural Science Foundation of China(NSFC)(No.81701573)Key Technologies of Prevention and Control for Major and Infectious Diseases Project of Suzhou(No.GWZX201604)Youth Medical Talent Project of Jiangsu(No.QNRC2016214)。
文摘Alcoholic liver disease(ALD) has become one of the leading causes of death in the world. Berbamine(BM), a natural product mainly derived from Berberis vulgaris L, possesses multiple bioactivities as a traditional medicine. However, the protective effect of BM on ALD remains unknown. In this study, we investigated the effect of BM on ethanol-induced hepatic injury in mice and its underlying mechanism. It was shown that BM at 0.3125-40 μmol·L^-1 had no effect on macrophages and hepatocytes proliferation. BM at 5-20 μmol·L^-1 significantly inhibited lipopolysaccharide(LPS) or acetate-induced IL-1β and IL-6 m RNA expression in RAW264.7 cells. Moreover, BM treatment significantly inhibited LPS-induced p65 and STAT3 phosphorylation in RAW264.7 cells. Hepatic histopathology analysis showed that inflammatory cells infiltration and lipid accumulation were suppressed by 25 and 50 mg·kg^-1 BM administration in ethanol-induced hepatic injury mouse model. Meanwhile, BM treatment significantly inhibited serum ALT and AST levels in ethanol-fed mice. Oil red O staining results showed that BM administration ameliorated hepatic lipid accumulation in ethanolfed mice. Preventions of ethanol-induced hepatic injury by BM were reflected by markedly decreased serum and hepatic triglyceride(TG) and total cholesterol(TC) contents. Real-time PCR results showed that BM treatment significantly inhibited pro-inflammatory cytokines m RNA expression in ethanol-fed mouse liver. Remarkably, the mechanism of action of BM was related to the reduction of ethanol-induced NF-κB and STAT3 phosphorylation levels in liver. In addition, BM treatment significantly inhibited ERK phosphorylation but not JNK and p38 of MAPK pathway. Taken together, our results demonstrate a beneficial effect of BM on ethanol-induced liver injury via a mechanism associated with inactivation of NF-κB, STAT3 and ERK pathway, which gives insight into the further evaluation of the therapeutic potential of BM for ALD.
