OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice

As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor OsbZIP01,which can suppress the expression of SD1 and regulate gibberellin(GA)biosynthesis in rice.Knockout mutants of OsbZIP01 exhibited increased plant height,while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate.Furthermore,the semi-dwarf phenotype of OE-bZIP01,was caused by the reduced internode length,which was accompanied by a thin stem width.The predominant expression of OsbZIP01 was observed in leaves and sheaths.OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity.In addition,OsbZIP01 could directly bind to the promoter of the OsSD1 gene,and inhibit its transcription.The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA_(3).Meanwhile,the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type,indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway.Collectively,OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription,thereby affecting plant growth and development.

不同取代度乙酰化淀粉的制备、表征及热塑性分析

为了使淀粉作为高分子材料更好地应用在材料行业,需要将淀粉进行改性,本研究以木薯淀粉为原料,依次对其进行预处理和无水介质的乙酰化处理,通过控制反应时间的变化,制备了不同取代度乙酰化淀粉。并且通过X射线衍射、偏光显微镜、扫描电子显微镜、接触角测量、差示扫描量热分析等表征手段探究了预处理对乙酰化淀粉颗粒理化性质的影响、高取代度淀粉与低取代度淀粉理化性质的差异。结果表明:预处理可缩短原淀粉乙酰化所需时间,高取代度乙酰化淀粉晶体衍射峰消失,偏光十字消失,形貌由圆形变为多孔状,可以在丙酮等有机溶剂中溶解,预处理淀粉乙酰化反应0.5 h其疏水接触角就可达69.006°±0.520°,淀粉熔融温度降到155℃,分解温度由280℃增加到340℃,使淀粉热稳定性增强,具有热塑性。该研究可在半小时内制备出取代度为2.5的乙酰化淀粉,可以溶解在有机溶剂中,为淀粉在材料行业的应用提供了新思路。

能量有效的无线传感器网络分簇路由协议

无线传感器网络(WSNs)的应用前景非常广阔,得到了越来越广泛的关注,其中网络节能、降低功耗、延长寿命是WSNs必须解决的问题。对WSNs中典型的分簇路由协议进行了详细分析,通过对比研究,提出了一种基于节点间相关性的能量有效分簇路由协议——BCCP协议。在BCCP协议中,能耗均衡分簇算法利用节点间位置相关性与节点剩余能量,降低分簇迭代次数,得到分布均匀的簇首节点,降低簇内成员节点与簇首节点的通信能耗,以此降低网络能耗;降低能耗分簇算法利用节点间数据相似性与节点间协同性,降低簇内通信、簇间通信的数据量,以此降低网络能耗。实验结果表明,BCCP协议在能耗均衡、网络存活节点数、降低能耗方面与其他方法相比均有明显的优势。

法匹拉韦治疗新型冠状病毒的应用评价与药学监护

法匹拉韦是一种新型广谱抗病毒药物,本文从法匹拉韦的药理作用与药代动力学特征、临床应用、治疗新型冠状病毒肺炎的理论依据进行分析和总结,为临床上应用法匹拉韦治疗新型冠状病毒肺炎的安全性及有效性提供参考,提出对不同人群给予合理用药和监护建议。法匹拉韦治疗新型冠状病毒肺炎有一定作用,其适应证、最佳使用剂量、特殊人群用药及联合用药上还需要更多的研究和探索。