目的探讨手足口病(hand,foot and mouth disease,HFMD)患儿细胞免疫和体液免疫指标改变的临床意义。方法80例HFMD患儿根据卫健委手足口病预防控制指南分为轻型组38例和重症组42例,选取同年龄段正常体检儿童40例为对照组,检测外周静脉血...目的探讨手足口病(hand,foot and mouth disease,HFMD)患儿细胞免疫和体液免疫指标改变的临床意义。方法80例HFMD患儿根据卫健委手足口病预防控制指南分为轻型组38例和重症组42例,选取同年龄段正常体检儿童40例为对照组,检测外周静脉血T淋巴细胞亚群、血清免疫球蛋白、血清补体及炎症细胞因子。结果HFMD轻型组和重症组患儿外周血T淋巴细胞亚群(CD3^(+)、CD4^(+)和CD8^(+)细胞)检测值均低于正常儿童,差异有统计学意义(P<0.01),重症组低于轻型组(P<0.05)。HFMD轻型组和重症组患儿血清免疫球蛋白(IgG、IgA和IgM)、血清炎症细胞因子(IL-1、IL-6和TNF-α)水平高于正常儿童且随病情加重升高(P<0.01),INF及C3和C4含量低于正常儿童且随病情加重下降(P<0.01)。结论HFMD患儿细胞免疫和体液免疫指标较正常儿童均改变且随病情加重变化幅度增加,可为临床判断病情严重程度及采取干预措施提供一定参考依据。展开更多
Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice ...Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice were allocated into 6 groups by a random number table,including control,LPS,dexamethasone(Dex,1 mg/kg),and 120,240 and 480 mg/kg SBE groups,10 mice in each group.Except the control group,mice were pre-treated with Dex and SBE,respectively,for 7 days before LPS was intraperitoneally injected to induce ALl.Pulmonary vascular hyperpermeabilitywas evaluated by histopathologic observation and transvascular leakagedetermination.Tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6)levels in serum were measured using enzyme-linked immunosorbent assay.The expression of nuclear factor-kappa B(NF-k B)p65 in lung cells was determined by immunofluorescence analysis.The contents of cytoplasmic inhibitorof nuclear factor-kBkinase(IKK)and nuclearp65,as wellasdownstreamproteins of E-selectin(CD62E)and intercellular adhesion molecule-1(ICAM-1),were determined using Western blot analysis.Results:Histopathological observation confirmed SBEtreatment alleviatedmorphological lesion induced by LPS.Compared with the LPS group,480 mg/kg SBE significantly decreased the water content of lung,Evans blue accumulation in lung tissue,and protein concentration and neutrophils count in bronchoalveolar lavage fluid(P<o.01);moreover,480 mg/kg SBE significantly suppressed release of TNF-αand IL-6,and down-regulated expressions of IKK,nuclear p65,ICAM-1 and CD62E(P<0.01).Conclusion:SBE maintained alveolar-capillary barrier integrity under endotoxin challenge in mice by suppressing the key factors in the pathogenesis of ALl.展开更多
文摘目的探讨手足口病(hand,foot and mouth disease,HFMD)患儿细胞免疫和体液免疫指标改变的临床意义。方法80例HFMD患儿根据卫健委手足口病预防控制指南分为轻型组38例和重症组42例,选取同年龄段正常体检儿童40例为对照组,检测外周静脉血T淋巴细胞亚群、血清免疫球蛋白、血清补体及炎症细胞因子。结果HFMD轻型组和重症组患儿外周血T淋巴细胞亚群(CD3^(+)、CD4^(+)和CD8^(+)细胞)检测值均低于正常儿童,差异有统计学意义(P<0.01),重症组低于轻型组(P<0.05)。HFMD轻型组和重症组患儿血清免疫球蛋白(IgG、IgA和IgM)、血清炎症细胞因子(IL-1、IL-6和TNF-α)水平高于正常儿童且随病情加重升高(P<0.01),INF及C3和C4含量低于正常儿童且随病情加重下降(P<0.01)。结论HFMD患儿细胞免疫和体液免疫指标较正常儿童均改变且随病情加重变化幅度增加,可为临床判断病情严重程度及采取干预措施提供一定参考依据。
基金Supported by the National Natural Science Foundation of China(No.81503361)。
文摘Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice were allocated into 6 groups by a random number table,including control,LPS,dexamethasone(Dex,1 mg/kg),and 120,240 and 480 mg/kg SBE groups,10 mice in each group.Except the control group,mice were pre-treated with Dex and SBE,respectively,for 7 days before LPS was intraperitoneally injected to induce ALl.Pulmonary vascular hyperpermeabilitywas evaluated by histopathologic observation and transvascular leakagedetermination.Tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6)levels in serum were measured using enzyme-linked immunosorbent assay.The expression of nuclear factor-kappa B(NF-k B)p65 in lung cells was determined by immunofluorescence analysis.The contents of cytoplasmic inhibitorof nuclear factor-kBkinase(IKK)and nuclearp65,as wellasdownstreamproteins of E-selectin(CD62E)and intercellular adhesion molecule-1(ICAM-1),were determined using Western blot analysis.Results:Histopathological observation confirmed SBEtreatment alleviatedmorphological lesion induced by LPS.Compared with the LPS group,480 mg/kg SBE significantly decreased the water content of lung,Evans blue accumulation in lung tissue,and protein concentration and neutrophils count in bronchoalveolar lavage fluid(P<o.01);moreover,480 mg/kg SBE significantly suppressed release of TNF-αand IL-6,and down-regulated expressions of IKK,nuclear p65,ICAM-1 and CD62E(P<0.01).Conclusion:SBE maintained alveolar-capillary barrier integrity under endotoxin challenge in mice by suppressing the key factors in the pathogenesis of ALl.
