Nitric oxide (NO) is a multifunctional messen-ger molecule produced through oxidation of L-arginine to L-citrulline by enzyme NO synthase (NOS). In the current study, mouse blastocysts were cultured in the different m...Nitric oxide (NO) is a multifunctional messen-ger molecule produced through oxidation of L-arginine to L-citrulline by enzyme NO synthase (NOS). In the current study, mouse blastocysts were cultured in the different media, and the implantation capacity of blastocyst was evaluated by evaluating the percentage of embryos adhesion and out-growth after culture for 12, 24 or 48 h. Matrix metallopro-teinase-2 (MMP-2) mRNA was detected by RT-PCR, and MMP-2 protein was detected by gelatin zymography. Inhibi-tion of blastocyst adhesion and outgrowth was observed in embryo cultured with 500 mmol/L NOS inhibitor NG-mono- methyl-L-arginine (L-NMMA) alone; however, 100 mmol/L S-nitroso-Nacetylpenicillamine (SNAP), a NO donor, and 20 mmol/L cGMP analogue, 8-Br-cGMP could block this inhibi-tion. The expression and production of MMP-2 in the blas-tocysts were suppressed by L-NMMA, and SNAP or 8-br-cGMP could reverse this suppression. These results suggest that NO induces embryo implantation by cGMP sig-naling pathway.展开更多
MMPs and their natural tissue inhibitors TIMPs are crucial in coordinated breakdown and remodel- ing of the extracellular matrix (ECM) in physiological and pathological situations. Placentation is a key event of pregn...MMPs and their natural tissue inhibitors TIMPs are crucial in coordinated breakdown and remodel- ing of the extracellular matrix (ECM) in physiological and pathological situations. Placentation is a key event of pregnancy in which MMPs/TIMPs system plays important roles in regulating the extravillus cytotrophoblast (EVTs) invasion. This paper focuses on expression patterns and regulatory mechanisms of MMPs/TIMPs family members during the process of placentation. Their implications in curing pregnancy-related diseases are also discussed.展开更多
文摘Nitric oxide (NO) is a multifunctional messen-ger molecule produced through oxidation of L-arginine to L-citrulline by enzyme NO synthase (NOS). In the current study, mouse blastocysts were cultured in the different media, and the implantation capacity of blastocyst was evaluated by evaluating the percentage of embryos adhesion and out-growth after culture for 12, 24 or 48 h. Matrix metallopro-teinase-2 (MMP-2) mRNA was detected by RT-PCR, and MMP-2 protein was detected by gelatin zymography. Inhibi-tion of blastocyst adhesion and outgrowth was observed in embryo cultured with 500 mmol/L NOS inhibitor NG-mono- methyl-L-arginine (L-NMMA) alone; however, 100 mmol/L S-nitroso-Nacetylpenicillamine (SNAP), a NO donor, and 20 mmol/L cGMP analogue, 8-Br-cGMP could block this inhibi-tion. The expression and production of MMP-2 in the blas-tocysts were suppressed by L-NMMA, and SNAP or 8-br-cGMP could reverse this suppression. These results suggest that NO induces embryo implantation by cGMP sig-naling pathway.
基金supported by the Special Funds for Major State Basic Research Project(Grant No.G1999055903)Funds from CAS(Grant No.KSCX2-SW-322)trom NSFC(Grant No.30170112).
文摘MMPs and their natural tissue inhibitors TIMPs are crucial in coordinated breakdown and remodel- ing of the extracellular matrix (ECM) in physiological and pathological situations. Placentation is a key event of pregnancy in which MMPs/TIMPs system plays important roles in regulating the extravillus cytotrophoblast (EVTs) invasion. This paper focuses on expression patterns and regulatory mechanisms of MMPs/TIMPs family members during the process of placentation. Their implications in curing pregnancy-related diseases are also discussed.
