Gold nanorods,as an emerging noble metal nanomaterial with unique properties,have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods...Gold nanorods,as an emerging noble metal nanomaterial with unique properties,have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods,especially their biocompatibility, optical property,and photothermal effects,have been attracting more and more attention.Gold nanorods exhibit great potential in applications such as tumor molecular imaging and photothermal therapy.In this article,we review some of the main advances made over the past few years in the application of gold nanorods in surface functionalization,molecular imaging,and photothermal therapy. We also explore other prospective applications and discuss the corresponding concepts,issues,approaches,and challenges,with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application.展开更多
Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent ...Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent magnetic nanoparticles(FMNPs) for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods: Human i PS cells were prepared and cultured for 72 h. The culture medium was collected, and then was coincubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human i PS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was observed using a small-animal imaging system. The nude mice were irradiated under an external alternating magnetic field and evaluated using an infrared thermal mapping instrument. Tumor sizes were measured weekly. Results: iP S cells and the collected culture medium inhibited the growth of MGC803 cells. FMNP-labeled human iP S cells targeted and imaged gastric cancer cells in vivo, as well as inhibited cancer growth in vivo through the external magnetic field. Conclusion: FMNP-labeled human i PS cells exhibit considerable potential in applications such as targeted dual-mode imaging and synergistic therapy for early gastric cancer.展开更多
Gastrointestinal cancers have become an important cause of cancer-related death in humans.Improving the early diagnosis rate of gastrointestinal tumors and improving the effect of surgical treatment can significantly ...Gastrointestinal cancers have become an important cause of cancer-related death in humans.Improving the early diagnosis rate of gastrointestinal tumors and improving the effect of surgical treatment can significantly improve the survival rate of patients.The conventional diagnostic method is high-definition white-light endoscopy,which often leads to missed diagnosis.For surgical treatment,intraoperative tumor localization and post-operative anastomotic state evaluation play important roles in the effect of surgical treatment.As a new imaging method,near-infrared fluorescence imaging(NIRFI)has its unique advantages in the diagnosis and auxiliary surgical treatment of gastrointestinal tumors due to its high sensitivity and the ability to image deep tissues.In this review,we focus on the latest advances of NIRFI technology applied in early diagnosis of gastrointestinal tumors,identification of tumor margins,identification of lymph nodes,and assessment of anastomotic leakage.In addition,we summarize the advances of NIRFI systems such as macro imaging and micro imaging systems,and also clearly describe the application process of NIRFI from system to clinical application,and look into the prospect of NIRFI applied in the theranostics of gastrointestinal tumors.展开更多
The methylcytosine dioxygenases TET proteins (TET1, TET2, and TET3) play important regulatory roles in neural function. In this study, we investigated the role of TET proteins in neuronal differentiation using Neuro...The methylcytosine dioxygenases TET proteins (TET1, TET2, and TET3) play important regulatory roles in neural function. In this study, we investigated the role of TET proteins in neuronal differentiation using Neuro2a cells as a model. We observed that knockdown of TET1, TET2 or TET3 promoted neuronal differentiation of Neuro2a cells, and their overexpression inhibited VPA (valproic acid)-induced neuronal differentiation, suggesting all three TET proteins negatively regulate neu- ronal differentiation of Neuro2a cells. Interestingly, the inducing activity of TET protein is independent of its enzymatic activity. Our previous studies have demon- strated that srGAP3 can negatively regulate neuronal differentiation of Neuro2a cells. Furthermore, we revealed that TET1 could positively regulate srGAP3 expression independent of its catalytic activity, and srGAP3 is required for TET-mediated neuronal differentiation of Neuro2a cells. The results presented here may facilitate better understanding of the role of TET proteins in neuronal differentiation, and provide a possible therapy target for neuroblastoma.展开更多
文摘Gold nanorods,as an emerging noble metal nanomaterial with unique properties,have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods,especially their biocompatibility, optical property,and photothermal effects,have been attracting more and more attention.Gold nanorods exhibit great potential in applications such as tumor molecular imaging and photothermal therapy.In this article,we review some of the main advances made over the past few years in the application of gold nanorods in surface functionalization,molecular imaging,and photothermal therapy. We also explore other prospective applications and discuss the corresponding concepts,issues,approaches,and challenges,with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application.
基金supported by National Natural Science Foundation of China (Grant No. 81225010, 20803040, 81028009, and 31170961)National Key Basic Research Program of China (973 Program) (Grant No. 2010CB933902 and 2015CB931802)+1 种基金National Key Technology Research and Development Program (863 Program) (Grant No. 2012AA022703 and 2014AA020700)Shanghai Science and Technology Fund (Grant No.13NM1401500)
文摘Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent magnetic nanoparticles(FMNPs) for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods: Human i PS cells were prepared and cultured for 72 h. The culture medium was collected, and then was coincubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human i PS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was observed using a small-animal imaging system. The nude mice were irradiated under an external alternating magnetic field and evaluated using an infrared thermal mapping instrument. Tumor sizes were measured weekly. Results: iP S cells and the collected culture medium inhibited the growth of MGC803 cells. FMNP-labeled human iP S cells targeted and imaged gastric cancer cells in vivo, as well as inhibited cancer growth in vivo through the external magnetic field. Conclusion: FMNP-labeled human i PS cells exhibit considerable potential in applications such as targeted dual-mode imaging and synergistic therapy for early gastric cancer.
基金financially supported by the International Cooperation Project of National Natural Science Foundation of China(No.82020108017)Innovation Group Project of National Natural Science Foundation of China(No.81921002)+2 种基金the National Key Research and Development Project of China(No.2017YFA0205301)Projects of Shanghai Science and Technology Commission(21DZ2203200,20142201300,20142200900)China Postdoctoral Science Foundation(Grant No.2020M671130).
文摘Gastrointestinal cancers have become an important cause of cancer-related death in humans.Improving the early diagnosis rate of gastrointestinal tumors and improving the effect of surgical treatment can significantly improve the survival rate of patients.The conventional diagnostic method is high-definition white-light endoscopy,which often leads to missed diagnosis.For surgical treatment,intraoperative tumor localization and post-operative anastomotic state evaluation play important roles in the effect of surgical treatment.As a new imaging method,near-infrared fluorescence imaging(NIRFI)has its unique advantages in the diagnosis and auxiliary surgical treatment of gastrointestinal tumors due to its high sensitivity and the ability to image deep tissues.In this review,we focus on the latest advances of NIRFI technology applied in early diagnosis of gastrointestinal tumors,identification of tumor margins,identification of lymph nodes,and assessment of anastomotic leakage.In addition,we summarize the advances of NIRFI systems such as macro imaging and micro imaging systems,and also clearly describe the application process of NIRFI from system to clinical application,and look into the prospect of NIRFI applied in the theranostics of gastrointestinal tumors.
文摘The methylcytosine dioxygenases TET proteins (TET1, TET2, and TET3) play important regulatory roles in neural function. In this study, we investigated the role of TET proteins in neuronal differentiation using Neuro2a cells as a model. We observed that knockdown of TET1, TET2 or TET3 promoted neuronal differentiation of Neuro2a cells, and their overexpression inhibited VPA (valproic acid)-induced neuronal differentiation, suggesting all three TET proteins negatively regulate neu- ronal differentiation of Neuro2a cells. Interestingly, the inducing activity of TET protein is independent of its enzymatic activity. Our previous studies have demon- strated that srGAP3 can negatively regulate neuronal differentiation of Neuro2a cells. Furthermore, we revealed that TET1 could positively regulate srGAP3 expression independent of its catalytic activity, and srGAP3 is required for TET-mediated neuronal differentiation of Neuro2a cells. The results presented here may facilitate better understanding of the role of TET proteins in neuronal differentiation, and provide a possible therapy target for neuroblastoma.