Repeatability,interocular correlation and agreement of optic nerve head vessel density in healthy eyes:a sweptsource optical coherence tomographic angiography study

AIM:To assess the repeatability,interocular correlation,and agreement of quantitative swept-source optical coherence tomography angiography(OCTA)optic nerve head(ONH)parameters in healthy subjects.METHODS:Thir ty-three healthy subjects were enrolled.The ONH of both eyes were imaged four times by a swept-source-OCTA using a 3 mm×3 mm scanning protocol.Images of the radial peripapillary capillary were analyzed by a customized Matlab program,and the vessel density,fractal dimension,and vessel diameter index were measured.The repeatability of the four scans was determined by the intraclass correlation coefficient(ICC).The most well-centered optic disc from the four repeated scans was then selected for the interocular correlation and agreement analysis using the Pearson correlation coefficient,ICC and Bland-Altman plots.RESULTS:All swept-source-OCTA ONH parameters exhibited certain repeatability,with ICC>0.760 and coefficient of variation(CoV)≤7.301%.The obvious interocular correlation was observed for papillary vessel density(ICC=0.857),vessel diameter index(ICC=0.857)and fractal dimension(ICC=0.906),while circumpapillary vessel density exhibited moderate interocular correlation(ICC=0.687).Bland-Altman plots revealed an agreement range of-5.26%to 6.21%for circumpapillary vessel density.CONCLUSION:OCTA ONH parameters demonstrate good repeatability in healthy subjects.The interocular correlations of papillary vessel density,fractal dimension and vessel diameter index are high,but the correlation for circumpapillary vessel density is moderate.

Mitochondrial dysfunction in type 2 diabetes:A neglected path to skeletal muscle atrophy

Over the course of several decades,robust research has firmly established the significance of mitochondrial pathology as a central contributor to the onset of skeletal muscle atrophy in individuals with diabetes.However,the specific intricacies governing this process remain elusive.Extensive evidence highlights that individuals with diabetes regularly confront the severe consequences of skeletal muscle degradation.Deciphering the sophisticated mechanisms at the core of this pathology requires a thorough and meticulous exploration into the nuanced factors intricately associated with mitochondrial dysfunction.

Color gradients of spiral disks in the Sloan Digital Sky Survey

We investigate the radial color gradients of galactic disks using a sample of - 20 000 face-on spiral galaxies selected from the fourth data release of the Sloan Digital Sky Survey （SDSS-DR4）. We combine galaxies with similar concentrations, sizes and luminosities to construct composite galaxies, and then measure their color profiles by stacking the azimuthally averaged radial color profiles of all the member galaxies. Except for the smallest galaxies （R50 〈 3 kpc）, almost all galaxies show negative disk color gradients with mean 9 - r gradient Ggr = -0.006 magkpc-1 and r - z gradient Grz = -0.018 mag kpc^-1. The disk color gradients are independent of the morphological types of galaxies and strongly dependent on the disk surface brightness μd, with lower surface brightness galactic disks having steeper color gradients. We quantify the intrinsic correlation between color gradients and surface brightness as Ggr = -0.011μd ＋ 0.233 and Grz - -0.015μd ＋ 0.324. These quantified correlations provide tight observational constraints on the formation and evolution models of spiral galaxies.

Estimating Photometric Redshifts with Artificial Neural Networks and Multi-Parameters

We calculate photometric redshifts from the Sloan Digital Sky Survey Data Release 2 （SDSS DR2） Galaxy Sample using artificial neural networks （ANNs）. Different input sets based on various parameters （e.g. magnitude, color index, flux information） are explored. Mainly, parameters from broadband photometry are utilized and their performances in redshift prediction are compared. While any parameter may be easily incorporated in the input, our results indicate that using the dereddened magnitudes often produces more accurate photometric redshifts than using the Petrosian magnitudes or model magnitudes as input, but the model magnitudes are superior to the Petrosian magnitudes. Also, better performance resuits when more effective parameters are used in the training set. The method is tested on a sample of 79 346 galaxies from the SDSS DR2. When using 19 parameters based on the dereddened magnitudes, the rms error in redshift estimation is σz = 0.020184. The ANN is highly competitive tool compared to the traditional template-fitting methods when a large and representative training set is available.

Periarthritis of shoulder treated with Fu’s subcutaneous needling and electroacupuncture:A controlled trail

Objective:To observe and compare the therapeutic effect on shoulder periarthritis between Fu’s subcutaneous needling(FSN)and electroacupuncture(EA).Methods:A total of 68 patients with periarthritis of shoulder were divided into a FSN group and an EA group according to the random number table,34 cases in each one.In the FSN group,FSN was adopted,once every two days,totally for 10 times.In the EA group,EA was used once daily,consecutively for 20 times.Shoulder pain score and shoulder function score(Constant-Murley score)before and after treatment were compared between the two groups.The clinical therapeutic effect was analyzed and evaluated statistically.Results:After 20-day treatment,shoulder pain score was reduced obviously as compared with that before treatment in the patients of either group(both P<0.01).After treatment,shoulder pain score in the FSN group was lower significantly than that in the EA group(P<0.01).In 20 days of treatment,CMS was improved obviously as compared with that before treatment in the patients of both groups(both P<0.01)and CMS in the FSN group was higher significantly than that in the EA group after treatment(P<0.01).The effective rate was 94.11%in the FSN group and was 91.17%in the EA group.The remarkably effective rate was 73.52%in the FSN group,significantly higher than 20.58%in the EA group(P<0.01).Conclusion:Both FSN and EA are effective in treatment of periarthritis of shoulder,but FSN obtains a much higher remarkably effective rate in treatment of periarthritis of shoulder and its effects for relieving shoulder pain and improving shoulder function are superior to EA.Because of its effective treatment and simple operation,FSN deserves to be promoted in clinical practice for periarthritis of shoulder.

Systematic Training of Liver Imaging Reporting and Data System Magnetic Resonance Imaging v2018 can Improve the Diagnosis of Hepatocellular Carcinoma for Different Radiologists

Background and Aims:Liver imaging reporting and data system(LI-RADS)provides standardized lexicon and categorization for diagnosing hepatocellular carcinoma(HCC).However,there is limited knowledge about the effect of LI-RADS training.We prospectively explored whether the systematic training of LI-RADS v2018 on magnetic resonance imaging(MRI)can effectively improve the diagnostic performances of different radiologists for HCC.Methods:A total of 20 visiting radiologists and the multiparametric MRI of 70 hepatic observations in 61 patients with high risk of HCC were included in this study.The LI-RADS v2018 training procedure included three times of thematic lectures(each lasting for 2.5 h)given by a professor specialized in imaging diagnosis of liver,with an interval of a month.After each seminar,the radiologists had a month to adopt the algorithm into their daily work.The diagnostic performances and interobserver agreements of these radiologists adopting the algorithm for HCC diagnosis before and after training were compared.Results:A total of 20 radiologists(male/female,12/8;with an average age of 36.75±4.99 years)were enrolled.After training,the interobserver agreements for the LI-RADS category for all radiologists(p=0.005)were increased.The sensitivity,specificity,positive predictive value,negative predictive value,and coincidence rate of all radiologists for HCC diagnosis before and after training were 43%vs.54%,86%vs.88%,74%vs.81%,62%vs.67%,and 65%vs.71%,respectively.The diagnostic performances of all radiologists(p<0.001)showed improvement after training.Conclusions:The systematic training of LI-RADS can effectively improve the diagnostic performances of radiologists with different experiences for HCC.

Ethnopharmacological Investigation and Rapid Authentication of Mongolian Patent Medicines Digeda

Objective To investigate Mongolian medicinal plants called Digeda and the prescriptions in Inner Mongolia region and to establish a molecular method for authentication of Digeda Mongolian patent medicines （MPMs）. Methods A field investigation was conducted on traditional uses of Digeda. After interviewed traditional healers in Mongolian, ethnopharmacological information of Digeda prescriptions was recorded in detail, including names, compositions, and traditional uses. And the total DNA from 10 MPMs has been amplified by three pairs of specific primers. Specific PCR products were further identified by sequence alignment with the known sequences already submitted in GenBank or own sequences. Results Fifteen Digeda plants and 29 Digeda prescriptions with their ethnopharmacological knowledge were collected. Ten MPM samples containing Lomatogonium rotatum, Viola philippica, and Corydalis bungeana were successfully evidenced by PCR with specific bands as raw materials. Conclusion Digeda should be further investigated in ethnopharmacology, which is a fundamental step toward developing efficacious natural drugs for various diseases. PCR amplification of specific allele is an easy and economical method, which can be used to identify highly processed MPMs and will assist in monitoring their qualities and legalities.

Transcriptome-wide association analysis identified candidate susceptibility genes for nasopharyngeal carcinoma

Dear Editor,Nasopharyngeal carcinoma(NPC)is a common malignancy in East and Southeast Asia,especially in South China.The etiology of NPC has been linked to genetic susceptibility,Epstein-Barr virus(EBV)infection,and environmental factors.Accumulated evidence including multiple genome-wide association studies(GWASs)has revealed robust genetic predisposition of NPC.However,GWAS-identified genetic variants collectively account for only 8.2%of NPC heritability[1].The underlying inherited predisposition is largely undetermined.The strongest genetic signal for NPC consistently hits the human leukocyte antigen(HLA)region on 6p21[2].However,the highly polymorphic nature and complicated long-range linkage disequilibrium(LD)in the HLA region particularly obscure the causal variants driving the association.In addition,most genetic variants located in introns or intergenic regions.The causal genes mediating genetic effects on NPC risk have rarely been ascertained by GWAS alone.