The genome of green microalgae has rarely been reported.Ulva prolifera is a green microalga that has received much attention.Despite research articles about U.prolifera in recent years,we know very little about its ge...The genome of green microalgae has rarely been reported.Ulva prolifera is a green microalga that has received much attention.Despite research articles about U.prolifera in recent years,we know very little about its genome.Therefore,the 87.9-Mb haploid genome(containing 10311 protein-coding genes)of U.prolifera was studied,and the genome was compared with that of U.mutabilis,which is the only published Ulva species.Results showed that the two species are closely related.A phylogenetic tree was constructed among U.prolifera and other green algae available in GenBank,revealing the evolutionary status of U.prolifera in Chlorophyta.To understand why U.prolifera could grow rapidly,we identified some genes related to growth,such as those involved in cell division,phosphorylation,and cell proliferation.In addition,genes related to stress resistance were found,which supports the notion that U.prolifera grows vigorously in nature.These results help to characterize green tides from a new perspective and reveal some important insight into the biology of U.prolifera.展开更多
microRNAs(miRNA)families play a critical role in plant growth,development,and responses to abiotic stress.In this study,we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen deprava...microRNAs(miRNA)families play a critical role in plant growth,development,and responses to abiotic stress.In this study,we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress.The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated.N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes,including cyclin A3 and cyclin L,which were strictly required for cell cycle progression.In addition,U.prolifera with highly expression of Up-miR-843 showed improved biomass,and photosynthesis compared with that under normal growth conditions.Thus,the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes,which further regulated the growth of U.prolifera.展开更多
基金Supported by the Natural Science Foundation of Jiangsu Province(No.BK20200882)the MNR Key Laboratory of Eco-environmental Science and Technology,China(No.MEEST-2020-2)+3 种基金the Chinese Post-doctoral Science Foundation(No.2020M681698)the Jiangsu Planned Projects for Postdoctoral Research Funds(No.2020Z300)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.20KJD170004)the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘The genome of green microalgae has rarely been reported.Ulva prolifera is a green microalga that has received much attention.Despite research articles about U.prolifera in recent years,we know very little about its genome.Therefore,the 87.9-Mb haploid genome(containing 10311 protein-coding genes)of U.prolifera was studied,and the genome was compared with that of U.mutabilis,which is the only published Ulva species.Results showed that the two species are closely related.A phylogenetic tree was constructed among U.prolifera and other green algae available in GenBank,revealing the evolutionary status of U.prolifera in Chlorophyta.To understand why U.prolifera could grow rapidly,we identified some genes related to growth,such as those involved in cell division,phosphorylation,and cell proliferation.In addition,genes related to stress resistance were found,which supports the notion that U.prolifera grows vigorously in nature.These results help to characterize green tides from a new perspective and reveal some important insight into the biology of U.prolifera.
基金Foundation item:The National Key R&D Program of China under contract No.2016YFC1402102the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘microRNAs(miRNA)families play a critical role in plant growth,development,and responses to abiotic stress.In this study,we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress.The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated.N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes,including cyclin A3 and cyclin L,which were strictly required for cell cycle progression.In addition,U.prolifera with highly expression of Up-miR-843 showed improved biomass,and photosynthesis compared with that under normal growth conditions.Thus,the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes,which further regulated the growth of U.prolifera.
