Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling

Objective:To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry(Morus alba) leaves using Cordyceps militais.Methods:Dried mulberry leaves,dried mulberry leaves with50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25℃,after which the dried mulberry leaves and fermented product were extracted with 70%ethanol and subjected to high performance liquid chromatography(HPLC).The contents of cordycepin,pelargonidin,chlorogenic acid,iso-quercetin and caffeic acid were determined.We then used the 3 T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro.Results:HPLC showed that fermentation changed the contents of cordycepin,pelargonidin,chlorogenic acid,iso-quercetin and caffeic acid.Furthermore,fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves,fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption.Additionally,fermented dried mulberry leaves with50% raw silkworm pupa inhibited PPAR-γ signaling.Conclusions:Fermentation with Cordyceps militaris enhanced antiadipogenesis efficacy of mulberry leaves.

Effects of aqueous extract from red Liriope platyphylla on phthalic-anhydride-induced atopic dermatitis in Interleukin-4/Luciferase/Consensus non-coding sequence-1 transgenic mice evaluated in terms of luciferase signal and general phenotype biomarkers

OBJECTIVE: To quantitatively evaluate the therapeutic effects of Red Liriope platyphylla(RLP) on atopic dermatitis(AD), alterations in the luciferase(Luc) signal and general phenotype biomarkers were compared in phthalic anhydride(PA) treated Interleukin-4(IL-4)/Luc/Consensus non-coding sequence-1(CNS-1) transgenic(Tg) mice following treatment with aqueous extract of RLP(AEt RLP) for4 weeks.METHODS: Alterations in AD phenotypes were measured in IL-4/Luc/CNS-1 Tg mice following treatment with AEt RLP using inflammation parameter analysis, bioluminescence imaging analysis, histological analysis, reverse transcription-polymerase chain reaction, enzyme linked immunosorbent assay and Western blot analysis.RESULTS: RLP contained high concentrations of total phenolic compounds, total flavonoid compounds and 5-HNE related to AD therapy. The Luc signal was only detected in the abdominal region and the submandibular lymph node(SL), mesenteric lymph node(ML), thymus and pancreas of the PA treated group. This signal was significantly decreased by 28%-73% throughout the body and in the four organs in PA + AEt RLP treated group. Furthermore, the lymph node weight, immunoglobulin E concentration and dermal thickness were decreased by 37%-67% in the PA + AEt RLP treated group.CONCLUSION: Our findings suggest that the therapeutic effect of AEt RLP on PA induced AD could be successfully quantified by comparison of Luc signals and AD phenotype markers in IL-4/Luc/CNS-1Tg mice, and that the Luc signal was as sensitive as the general AD phenotypes, enabling detection of effects without euthanasia.