Background Genome editing has been considered as powerful tool in agricultural fields.However,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestat...Background Genome editing has been considered as powerful tool in agricultural fields.However,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising cost.Furthermore,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in cattle.In this point of view,electroporation in embryos has been risen as an alternative.Results First,editing efficiency of our electroporation methods were tested for embryos.Presence of mutation on embryo was confirmed by T7E1 assay.With first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,respectively.In case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for BLG.Afterwards,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep sequencing.Thirteen recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double KO.Ten surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both genes.Conclusions These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively applied.Finally,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.展开更多
Recently, the popularity of 3D content is on the rise because of its immersive experience to view- ers. While demands for 3D contents and 3D technologies increase, only a few copyright protection methods for 3D conten...Recently, the popularity of 3D content is on the rise because of its immersive experience to view- ers. While demands for 3D contents and 3D technologies increase, only a few copyright protection methods for 3D contents have been proposed. The simplest infringement is the illegal distribution of the single 2D image from 3D content. The leaked image is still valuable as 2D content and the leakage can be occurred in DIBR system. To detect the leaked image, we focus on the hole-filled region which is caused by the hole-filling procedure mandatory in DIBR system. To estimate the hole-filled regions, two different procedures are conducted to extract edges and to estimate 3D warping traces, respectively. After that, the hole-filled regions are estimated and the left-right-eye image discrimination (LR discrimination) is also conducted. Experimental results demonstrate the effectiveness of the proposed method using quantitative measures.展开更多
基金financially supported by the National Research Foundation of Korea(NRF-2021R1A5A1033157 for SRC program:382 Comparative medicine Disease Research Center,NRF-2021R1F1A105195313)the Research Institute of Veterinary Science,the BK21 Four for Future Veterinary Medicine Leading Education and Research Center,and a Seoul National University(SNU)grant(#550e2020005)。
文摘Background Genome editing has been considered as powerful tool in agricultural fields.However,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising cost.Furthermore,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in cattle.In this point of view,electroporation in embryos has been risen as an alternative.Results First,editing efficiency of our electroporation methods were tested for embryos.Presence of mutation on embryo was confirmed by T7E1 assay.With first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,respectively.In case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for BLG.Afterwards,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep sequencing.Thirteen recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double KO.Ten surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both genes.Conclusions These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively applied.Finally,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.
文摘Recently, the popularity of 3D content is on the rise because of its immersive experience to view- ers. While demands for 3D contents and 3D technologies increase, only a few copyright protection methods for 3D contents have been proposed. The simplest infringement is the illegal distribution of the single 2D image from 3D content. The leaked image is still valuable as 2D content and the leakage can be occurred in DIBR system. To detect the leaked image, we focus on the hole-filled region which is caused by the hole-filling procedure mandatory in DIBR system. To estimate the hole-filled regions, two different procedures are conducted to extract edges and to estimate 3D warping traces, respectively. After that, the hole-filled regions are estimated and the left-right-eye image discrimination (LR discrimination) is also conducted. Experimental results demonstrate the effectiveness of the proposed method using quantitative measures.
