In this article,we propose a simple scheme of using berberine(BBR)to modify porous calcium phosphate ceramics(named PCPC).These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC.We found th...In this article,we propose a simple scheme of using berberine(BBR)to modify porous calcium phosphate ceramics(named PCPC).These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC.We found that these nanorods and the adsorbed BBR changed the interface micro-environment of PCPC by SEM images.The microenvironment of PCPC surface is essential for promoting BMSCs’proliferation and differentiation.These results demonstrated that PCPC/BBR markedly improved the bone regeneration of osteoporosis rats.Moreover,PCPC/BBR had significantly increased the expression levels of ALP,osteocalcin and bone morphogenetic protein2 and RUNX2 in BMSCs originated from osteoporosis rats.展开更多
Spinal cord injury(SCI)leads to nerve cell apoptosis and loss of motor function.Herein,excessive activation of the M1 phenotype macrophages/microglia is found to be the main reason for the poor prognosis of SCI,but th...Spinal cord injury(SCI)leads to nerve cell apoptosis and loss of motor function.Herein,excessive activation of the M1 phenotype macrophages/microglia is found to be the main reason for the poor prognosis of SCI,but the selective activation phenotype(M2)macrophages/microglia facilitates the recovery of SCI.Thereafter,we used gold nanoclusters loaded berberine(BRB-AuNCs)to reduce inflammation by inhibiting the activation of M1 phenotype macrophages/microglia,which simultaneously inhibited neuronal apoptosis after SCI.In vitro and in vivo experiments showed that BRB-AuNCs reduced M1 protein marker CD86,increased M2 protein marker CD206,reduced inflammation and apoptotic cytokines(IL-1β,IL-6,TNF-α,Cleaved Caspase-3 and Bax).These results indicate that BRB-AuNCs have excellent anti-inflammatory and anti-apoptotic effects by inducing the polarization of macrophages/microglia from M1 phenotype to M2 phenotype.Thereafter,the motor functions of SCI rats were significantly improved after treatment with BRB-AuNCs.This work not only provides a new way for the treatment of SCI but also broadens BRB utilization strategies.展开更多
基金This work was supported by National Natural Science Foundation of China(No.81771987,82072076,82072165 and 81871556)Young Top Talents in Xing Liao Talents Program of Liaoning Province(XLYC1807183).
文摘In this article,we propose a simple scheme of using berberine(BBR)to modify porous calcium phosphate ceramics(named PCPC).These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC.We found that these nanorods and the adsorbed BBR changed the interface micro-environment of PCPC by SEM images.The microenvironment of PCPC surface is essential for promoting BMSCs’proliferation and differentiation.These results demonstrated that PCPC/BBR markedly improved the bone regeneration of osteoporosis rats.Moreover,PCPC/BBR had significantly increased the expression levels of ALP,osteocalcin and bone morphogenetic protein2 and RUNX2 in BMSCs originated from osteoporosis rats.
基金supported by the National Natural Science Foundation of China(NSFC)(NO.81871556,82072165)Liaoning Revitalization Talents Program(NO.XLYC1902108).
文摘Spinal cord injury(SCI)leads to nerve cell apoptosis and loss of motor function.Herein,excessive activation of the M1 phenotype macrophages/microglia is found to be the main reason for the poor prognosis of SCI,but the selective activation phenotype(M2)macrophages/microglia facilitates the recovery of SCI.Thereafter,we used gold nanoclusters loaded berberine(BRB-AuNCs)to reduce inflammation by inhibiting the activation of M1 phenotype macrophages/microglia,which simultaneously inhibited neuronal apoptosis after SCI.In vitro and in vivo experiments showed that BRB-AuNCs reduced M1 protein marker CD86,increased M2 protein marker CD206,reduced inflammation and apoptotic cytokines(IL-1β,IL-6,TNF-α,Cleaved Caspase-3 and Bax).These results indicate that BRB-AuNCs have excellent anti-inflammatory and anti-apoptotic effects by inducing the polarization of macrophages/microglia from M1 phenotype to M2 phenotype.Thereafter,the motor functions of SCI rats were significantly improved after treatment with BRB-AuNCs.This work not only provides a new way for the treatment of SCI but also broadens BRB utilization strategies.
