Electronic fibers/textiles have great potential for applications in smart wearables due to their excellent flexibility,air permeability,and wearing comfort.However,it is still challenging to produce reliable electroni...Electronic fibers/textiles have great potential for applications in smart wearables due to their excellent flexibility,air permeability,and wearing comfort.However,it is still challenging to produce reliable electronic textiles at low cost and in a large scale.Herein,we report a spraying‐spinning process for fabricating electronic yarns with excellent stability and durability.Cotton sliver,which is the raw material for spinning conventional cotton yarns,was spray coated with carbon nanotubes(CNTs)and spun on an Ag@nylon yarn,forming a sheath‐core structured CNT@cotton‐Ag@nylon yarn(CCAY).The process is continuous,large‐scalable,applicable to other raw fiber materials and compatible with traditional textile processes.The as‐prepared CCAY showed superior mechanical durability,washability,and conductivity to typical surface coated yarns.It can be easily processed or integrated into textiles through weaving,knitting,sewing,and embroidering.We systematically studied the electromechanical,electro‐thermal,and photothermal performance of CCAY based yarns/fabrics,demonstrating its versatile applications in smart textiles.In addition,CCAY can be further equipped with other features,such as electro‐thermochromic functions,pH sensing and flame resistant abilities.Considering the large‐scalability,versatility,and low‐cost,we foresee that this spraying‐spinning process for electronic yarns may play important roles in the development of practical smart fibers/textiles.展开更多
DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously dev...DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.展开更多
基金study was supported by State Key Laboratory of New Textile Materials and Advanced Processing Tech-nologies,Wuhan Textile University(Nos.FZ2020009,FZ2021008)the National Natural Science Founda-tion of China(Nos.21975141,52125201).
文摘Electronic fibers/textiles have great potential for applications in smart wearables due to their excellent flexibility,air permeability,and wearing comfort.However,it is still challenging to produce reliable electronic textiles at low cost and in a large scale.Herein,we report a spraying‐spinning process for fabricating electronic yarns with excellent stability and durability.Cotton sliver,which is the raw material for spinning conventional cotton yarns,was spray coated with carbon nanotubes(CNTs)and spun on an Ag@nylon yarn,forming a sheath‐core structured CNT@cotton‐Ag@nylon yarn(CCAY).The process is continuous,large‐scalable,applicable to other raw fiber materials and compatible with traditional textile processes.The as‐prepared CCAY showed superior mechanical durability,washability,and conductivity to typical surface coated yarns.It can be easily processed or integrated into textiles through weaving,knitting,sewing,and embroidering.We systematically studied the electromechanical,electro‐thermal,and photothermal performance of CCAY based yarns/fabrics,demonstrating its versatile applications in smart textiles.In addition,CCAY can be further equipped with other features,such as electro‐thermochromic functions,pH sensing and flame resistant abilities.Considering the large‐scalability,versatility,and low‐cost,we foresee that this spraying‐spinning process for electronic yarns may play important roles in the development of practical smart fibers/textiles.
基金supported by the National Basic Research Program (2011CB911002)the National Natural Science Foundation of China (21190044, 21475037, 21222507, 21175036)the fundamental research funds for the central universities
文摘DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.
