Objective:To investigate the effect of asiatic acid(AA) on the differentiated human neuroblastoma SH-SY5 Y cells cytotoxic-induced by cholesterol.Methods:Human neuroblastoma SH-SY5 Y cells were either exposed to diffe...Objective:To investigate the effect of asiatic acid(AA) on the differentiated human neuroblastoma SH-SY5 Y cells cytotoxic-induced by cholesterol.Methods:Human neuroblastoma SH-SY5 Y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to reveal their responding viability by MTT assay.The selective 1 mmol/L concentration of AA was then used to test for either the protective or the recovery effects on the cells treated with 250 mmol/L concentration of cholesterol.Results:AA has a propensity to directly increase the viability of differentiated human neuroblastoma SH-SY5 Y cells.Cholesterol has significant cytotoxic effect on those cells in a concentration-dependent manner.AA has the ability to slightly recover the viability of the differentiated culture cytotoxic-induced by cholesterol but could not protect those cells from cytotoxic-induced by cholesterol.Conclusions:High concentrations of cholesterol were observed to be harmful to the neurons and AA had a slight effect of reducing neuronal death caused by cholesterol.展开更多
基金Supported by Naresuan University Research Fund for grants for this research(P2556C134)
文摘Objective:To investigate the effect of asiatic acid(AA) on the differentiated human neuroblastoma SH-SY5 Y cells cytotoxic-induced by cholesterol.Methods:Human neuroblastoma SH-SY5 Y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to reveal their responding viability by MTT assay.The selective 1 mmol/L concentration of AA was then used to test for either the protective or the recovery effects on the cells treated with 250 mmol/L concentration of cholesterol.Results:AA has a propensity to directly increase the viability of differentiated human neuroblastoma SH-SY5 Y cells.Cholesterol has significant cytotoxic effect on those cells in a concentration-dependent manner.AA has the ability to slightly recover the viability of the differentiated culture cytotoxic-induced by cholesterol but could not protect those cells from cytotoxic-induced by cholesterol.Conclusions:High concentrations of cholesterol were observed to be harmful to the neurons and AA had a slight effect of reducing neuronal death caused by cholesterol.
