In recent years,with the rise of video media such as YouTube and Tik Tok,the short video creation industry has emerged.How to spread China's excellent culture and how to“tell a good Chinese story”have become a h...In recent years,with the rise of video media such as YouTube and Tik Tok,the short video creation industry has emerged.How to spread China's excellent culture and how to“tell a good Chinese story”have become a hot concern.Using the 5W Model of Communication as a framework for analysis,this paper selects Chinese short videos with high popularity on YouTube as the object of the study.Studies found that the success of these videos was associated with five factors in the process of communication,which are communication main forces,communication content,communication channels,communication audience,and audience effects.Through theoretical and data analysis,the study concluded five strategies in video production to help promote Intercultural communication.展开更多
Partial hepatectomy(PH)can lead to severe complications,including liver failure,due to the low regenerative capacity of the remaining liver,especially after extensive hepatectomy.Liver sinusoidal endothelial cells(LSE...Partial hepatectomy(PH)can lead to severe complications,including liver failure,due to the low regenerative capacity of the remaining liver,especially after extensive hepatectomy.Liver sinusoidal endothelial cells(LSECs),whose proliferation occurs more slowly and later than hepatocytes after PH,compose the lining of the hepatic sinusoids,which are the smallest blood vessels in the liver.Vascular endothelial growth factor(VEGF),secreted by hepatocytes,promotes LSEC proliferation.Supplementation of exogenous VEGF after hepatectomy also increases the number of LSECs in the remaining liver,thus promoting the reestablishment of the hepatic sinusoids and accelerating liver regeneration.At present,some shortcomings exist in the methods of supplementing exogenous VEGF,such as a low drug concentration in the liver and the reaching of other organs.Moreover,VEGF should be administered multiple times and in large doses because of its short half-life.This review summarized the most recent findings on liver regeneration and new strategies for the localized delivery VEGF in the liver.展开更多
At first, the core ideology, advantage and principle of Software Testing Automation Framework (STAF) are presented in this paper. Several automated testing frameworks are summarized and analyzed. In addition, date dri...At first, the core ideology, advantage and principle of Software Testing Automation Framework (STAF) are presented in this paper. Several automated testing frameworks are summarized and analyzed. In addition, date driven automation test framework is given more attention. Test script is the important composing part of software test automation. Then this paper introduces several technologies of script along with their characteristics. Every technology applies to different places. Moreover, an automated test system with an automated test script language XML which is implemented to solve the problems in current automatic testing solutions. The design and implantation of the test script automation framework based on apache ant is put forward. The proposed key technology details of framework are also introduced. We take the automation test methodology of database connectivity operations by ODBC data source as example for validation, using auto test script as the key technology. Finally, the results demonstrate that it can best increase software test effectiveness and reduce workloads as well as save more efforts.展开更多
Based on reform of the supply front,this paper analyzes the main constraints on China's current structure of agricultural products,including irrational allocation of resources between agricultural products,promine...Based on reform of the supply front,this paper analyzes the main constraints on China's current structure of agricultural products,including irrational allocation of resources between agricultural products,prominent problems concerning agricultural product quality and safety,disconnection between market demand and agricultural product R&D,and low processing capacity of agricultural products. Based on summing up the experience of the countries with developed agriculture,this paper proposes the path and mechanism for the optimization of structure of agricultural products in China: optimizing resource allocation of agricultural products; improving the quality of agricultural products; enhancing the R&D capacity of agricultural enterprises; promoting the processing and conversion capacity of agricultural products; perfecting the policy support and subsidy mode.展开更多
An inexpensive phosphine catalyst was used effectively for a transition-metal-free acyl-transfer of N-containing heteroaryl ketones for the rapid synthesis of N-fused heterocycles.The key pre-aromatic spirocyclic inte...An inexpensive phosphine catalyst was used effectively for a transition-metal-free acyl-transfer of N-containing heteroaryl ketones for the rapid synthesis of N-fused heterocycles.The key pre-aromatic spirocyclic intermediate initialized by the single electron transfer(SET)process of Togni's reagentⅡpromoted by the tertiary phosphine resulted in an intriguing and alternative tactic for the cleavage of C–C bonds.By using inexpensive tertiary phosphine as the catalyst,this skeleton-reorganizing approach of N-containing heteroaryl ketones allows a streamlined assembly of complex N-fused heterocycles with broad functional group tolerance.展开更多
The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferat...The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental stresses.Emerging evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling pathways.Cancer cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor stimulation.This cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of macromolecules.The conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer cells.Moreover,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer development.The metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as exosome.Among them,cancer microenvironmental cells are immune cells which exert profound effects on cancer cells.Understanding of all these processes is a prerequisite for the development of a more effective strategy to contain cancers.展开更多
Additional sex combs-like 1(ASXL1)interacts with BRCA1-associated protein 1(BAP1)deubiquitinase to oppose the polycomb repressive complex 1(PRC1)-mediated histone H2A ubiquitylation.Germline BAP1 mutations are found i...Additional sex combs-like 1(ASXL1)interacts with BRCA1-associated protein 1(BAP1)deubiquitinase to oppose the polycomb repressive complex 1(PRC1)-mediated histone H2A ubiquitylation.Germline BAP1 mutations are found in a spectrum of human malignancies,while ASXL1 mutations recurrently occur in myeloid neoplasm and are associated with poor prognosis.Nearly all ASXL1 mutations are heterozygous frameshift or nonsense mutations in the middle or to a less extent the C-terminal region,resulting in the production of C-terminally truncated mutant ASXL1 proteins.How ASXL1 regulates specific target genes and how the C-terminal truncation of ASXL1 promotes leukemogen-esis are unclear.Here,we report that ASXL1 interacts with forkhead transcription factors FOXK1 and FOXK2 to regulate a subset of FOXK1/K2 target genes.We show that the C-terminally truncated mutant ASXL1 proteins are expressed at much higher levels than the wild-type protein in ASXL1 heterozygous leukemia cells,and lose the ability to interact with FOXK1/K2.Specific deletion of the mutant allele eliminates the expression of C-termi-nally truncated ASXL1 and increases the association of wild-type ASXL1 with BAP1,thereby restoring the expression of BAP1-ASXL1-FOXK1/K2 target genes,particularly those involved in glucose metabolism,oxygen sensing,and JAK-STAT3 signaling pathways.In addition to FOXK1/K2,we also identify other DNA-bind-ing transcription regulators including transcription factors(TFs)which interact with wild-type ASXL1,but not C-terminally truncated mutant.Our results suggest that ASXL1 mutations result in neomorphic alleles that contribute to leukemogenesis at least in part through dominantly inhibiting the wild-type ASXL1 from interacting with BAP1 and thereby impairing the function of ASXL1-BAP1-TF in regulating target genes and leukemia cell growth.展开更多
To the Editor:Identifying the pathogen from clinical samples is crucial for the diagnosis of a newly emergent infectious disease,such as 2019 novel coronavirus(2019-nCoV).which has posed great threats to global public...To the Editor:Identifying the pathogen from clinical samples is crucial for the diagnosis of a newly emergent infectious disease,such as 2019 novel coronavirus(2019-nCoV).which has posed great threats to global public health.[1]In some cases,despite the positive epidemiological,clinical,and radiographic evidence,coronavirus disease 2019(COVID-19)diagnosis can still be restricted by inconclusive polymerase chain reaction(PCR)results.[2]For samples of suspicious patients,where the fluorescence quantitative PCR(FQ-PCR)results in a few respiratory tract specimens were inconclusive,high-throughput sequencing(HTS)can be an effective confirmation method.Here,we report our experience of applying HTS to confirm a suspected 2019-nCoV infection.展开更多
基金supported by 2023 Wenzhou Philosophy and Social Science Planning Annual Project(2023年温州市社科联项目)(23WSK131YBM)Student Science Research Project of Wenzhou University(2023kx038)+2 种基金Student Innovation and Entrepreneurship Training Program Project of WZU(JWXC2023075)Zhejiang Foreign Language Society Project(ZWYB2023023)Zhejiang University Student Science and Technology Innovation Activity Plan(XMS2206006).
文摘In recent years,with the rise of video media such as YouTube and Tik Tok,the short video creation industry has emerged.How to spread China's excellent culture and how to“tell a good Chinese story”have become a hot concern.Using the 5W Model of Communication as a framework for analysis,this paper selects Chinese short videos with high popularity on YouTube as the object of the study.Studies found that the success of these videos was associated with five factors in the process of communication,which are communication main forces,communication content,communication channels,communication audience,and audience effects.Through theoretical and data analysis,the study concluded five strategies in video production to help promote Intercultural communication.
基金the Natural Science Foundation of Zhejiang Province,No.LQ21H030005
文摘Partial hepatectomy(PH)can lead to severe complications,including liver failure,due to the low regenerative capacity of the remaining liver,especially after extensive hepatectomy.Liver sinusoidal endothelial cells(LSECs),whose proliferation occurs more slowly and later than hepatocytes after PH,compose the lining of the hepatic sinusoids,which are the smallest blood vessels in the liver.Vascular endothelial growth factor(VEGF),secreted by hepatocytes,promotes LSEC proliferation.Supplementation of exogenous VEGF after hepatectomy also increases the number of LSECs in the remaining liver,thus promoting the reestablishment of the hepatic sinusoids and accelerating liver regeneration.At present,some shortcomings exist in the methods of supplementing exogenous VEGF,such as a low drug concentration in the liver and the reaching of other organs.Moreover,VEGF should be administered multiple times and in large doses because of its short half-life.This review summarized the most recent findings on liver regeneration and new strategies for the localized delivery VEGF in the liver.
文摘At first, the core ideology, advantage and principle of Software Testing Automation Framework (STAF) are presented in this paper. Several automated testing frameworks are summarized and analyzed. In addition, date driven automation test framework is given more attention. Test script is the important composing part of software test automation. Then this paper introduces several technologies of script along with their characteristics. Every technology applies to different places. Moreover, an automated test system with an automated test script language XML which is implemented to solve the problems in current automatic testing solutions. The design and implantation of the test script automation framework based on apache ant is put forward. The proposed key technology details of framework are also introduced. We take the automation test methodology of database connectivity operations by ODBC data source as example for validation, using auto test script as the key technology. Finally, the results demonstrate that it can best increase software test effectiveness and reduce workloads as well as save more efforts.
基金Supported by National Natural Science Foundation(71473205)Doctoral Fund Project of Southwest University(SWU1209338)
文摘Based on reform of the supply front,this paper analyzes the main constraints on China's current structure of agricultural products,including irrational allocation of resources between agricultural products,prominent problems concerning agricultural product quality and safety,disconnection between market demand and agricultural product R&D,and low processing capacity of agricultural products. Based on summing up the experience of the countries with developed agriculture,this paper proposes the path and mechanism for the optimization of structure of agricultural products in China: optimizing resource allocation of agricultural products; improving the quality of agricultural products; enhancing the R&D capacity of agricultural enterprises; promoting the processing and conversion capacity of agricultural products; perfecting the policy support and subsidy mode.
基金the financial support from the National Natural Science Foundation of China(Nos.21971205 and 22271231)the Natural Science Basic Research Plan in Shaanxi Province of China(No.2023-JC-YB-126)+1 种基金Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China(No.2022JC-08)Key Research and Invention Program in Shaanxi Province of China(No.2021SF-299)。
文摘An inexpensive phosphine catalyst was used effectively for a transition-metal-free acyl-transfer of N-containing heteroaryl ketones for the rapid synthesis of N-fused heterocycles.The key pre-aromatic spirocyclic intermediate initialized by the single electron transfer(SET)process of Togni's reagentⅡpromoted by the tertiary phosphine resulted in an intriguing and alternative tactic for the cleavage of C–C bonds.By using inexpensive tertiary phosphine as the catalyst,this skeleton-reorganizing approach of N-containing heteroaryl ketones allows a streamlined assembly of complex N-fused heterocycles with broad functional group tolerance.
文摘The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental stresses.Emerging evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling pathways.Cancer cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor stimulation.This cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of macromolecules.The conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer cells.Moreover,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer development.The metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as exosome.Among them,cancer microenvironmental cells are immune cells which exert profound effects on cancer cells.Understanding of all these processes is a prerequisite for the development of a more effective strategy to contain cancers.
基金the National Key R&D Program of China(No.2016YFA0501800 to D.Y.2016YFC1303303 to C.Y.)+5 种基金the NSFC Grant(No.31871431 and No.31821002 to D.Y.81572761,81772655 and 81972646 to Y.T.)the Innovative Research Team of High-Level Local Universities in Shanghai(to D.Y.)the Recruitment Program of Global Experts of China(Y.T.)the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning(V.T.)Shanghai Rising-Star Program(Y.T.).
文摘Additional sex combs-like 1(ASXL1)interacts with BRCA1-associated protein 1(BAP1)deubiquitinase to oppose the polycomb repressive complex 1(PRC1)-mediated histone H2A ubiquitylation.Germline BAP1 mutations are found in a spectrum of human malignancies,while ASXL1 mutations recurrently occur in myeloid neoplasm and are associated with poor prognosis.Nearly all ASXL1 mutations are heterozygous frameshift or nonsense mutations in the middle or to a less extent the C-terminal region,resulting in the production of C-terminally truncated mutant ASXL1 proteins.How ASXL1 regulates specific target genes and how the C-terminal truncation of ASXL1 promotes leukemogen-esis are unclear.Here,we report that ASXL1 interacts with forkhead transcription factors FOXK1 and FOXK2 to regulate a subset of FOXK1/K2 target genes.We show that the C-terminally truncated mutant ASXL1 proteins are expressed at much higher levels than the wild-type protein in ASXL1 heterozygous leukemia cells,and lose the ability to interact with FOXK1/K2.Specific deletion of the mutant allele eliminates the expression of C-termi-nally truncated ASXL1 and increases the association of wild-type ASXL1 with BAP1,thereby restoring the expression of BAP1-ASXL1-FOXK1/K2 target genes,particularly those involved in glucose metabolism,oxygen sensing,and JAK-STAT3 signaling pathways.In addition to FOXK1/K2,we also identify other DNA-bind-ing transcription regulators including transcription factors(TFs)which interact with wild-type ASXL1,but not C-terminally truncated mutant.Our results suggest that ASXL1 mutations result in neomorphic alleles that contribute to leukemogenesis at least in part through dominantly inhibiting the wild-type ASXL1 from interacting with BAP1 and thereby impairing the function of ASXL1-BAP1-TF in regulating target genes and leukemia cell growth.
基金This work was supported by grants from the National Key Research and Development Program of China(Nos.2018YFC1200100 and 2018YFC1311900)science research project of the Guangdong Province(No.2019B030316028)Guangzhou Medical University High-level University Clinical Research and Cultivation Program(Nos.[2017]159 and 160).
文摘To the Editor:Identifying the pathogen from clinical samples is crucial for the diagnosis of a newly emergent infectious disease,such as 2019 novel coronavirus(2019-nCoV).which has posed great threats to global public health.[1]In some cases,despite the positive epidemiological,clinical,and radiographic evidence,coronavirus disease 2019(COVID-19)diagnosis can still be restricted by inconclusive polymerase chain reaction(PCR)results.[2]For samples of suspicious patients,where the fluorescence quantitative PCR(FQ-PCR)results in a few respiratory tract specimens were inconclusive,high-throughput sequencing(HTS)can be an effective confirmation method.Here,we report our experience of applying HTS to confirm a suspected 2019-nCoV infection.
