The ability to expand genetic code in living cells has emerged as a powerful method with diverse applications.Here,we design re-placement of the anticodons of E.coli tRNAs that recognize codons for 20 natural amino ac...The ability to expand genetic code in living cells has emerged as a powerful method with diverse applications.Here,we design re-placement of the anticodons of E.coli tRNAs that recognize codons for 20 natural amino acids,with three anti-stop codons,resulting in a total of 60 engineered tRNA constructs.We test these constructs one by one in E.coli,and found that six tRNAsCUA(tyrV,serX,hisR,trpT,glnV and leuX),two tRNAsUCA(trpT and leuX)and one tRNAUUA(tyrV)allowed efficient expression of Red Fluorescence Protein(RFP)with the presence of a corresponding stop codon in frame.Furthermore,we exploit the mutual orthogonality of tRNASer CUA,tRNATrpUCA and tRNATyrUUA with corresponding stop codons and demonstrated that the tRNASer CUA and the tRNATrp UCA can provide dynamic range and low crosstalk.Finally,we show the TAG and TGA can not only be used as an"AND gate"circuit to control the translation of target gene,but also be used to control the translation of a prodeoxyviolacein(PDV)pathway and a reporter in parallel.Overall,this work provides flexible tools for translational control and holds great potential to promote synthetic biology studies.展开更多
基金The authors are grateful for the financial support fromthe National Key Research and Development Program of China(No.2018YFA0903700)the National Natural Science Foundation of China(Nos.31800719,21621004)the International(regional)cooperation and exchange projects(No.31861143017)。
文摘The ability to expand genetic code in living cells has emerged as a powerful method with diverse applications.Here,we design re-placement of the anticodons of E.coli tRNAs that recognize codons for 20 natural amino acids,with three anti-stop codons,resulting in a total of 60 engineered tRNA constructs.We test these constructs one by one in E.coli,and found that six tRNAsCUA(tyrV,serX,hisR,trpT,glnV and leuX),two tRNAsUCA(trpT and leuX)and one tRNAUUA(tyrV)allowed efficient expression of Red Fluorescence Protein(RFP)with the presence of a corresponding stop codon in frame.Furthermore,we exploit the mutual orthogonality of tRNASer CUA,tRNATrpUCA and tRNATyrUUA with corresponding stop codons and demonstrated that the tRNASer CUA and the tRNATrp UCA can provide dynamic range and low crosstalk.Finally,we show the TAG and TGA can not only be used as an"AND gate"circuit to control the translation of target gene,but also be used to control the translation of a prodeoxyviolacein(PDV)pathway and a reporter in parallel.Overall,this work provides flexible tools for translational control and holds great potential to promote synthetic biology studies.
