Over the past decades, the energy and concomitant environment issues, such as energy shortage, air pollution and global warming, have been becoming increasingly striking world-wide challenges [1,2]. Such a dilemma in ...Over the past decades, the energy and concomitant environment issues, such as energy shortage, air pollution and global warming, have been becoming increasingly striking world-wide challenges [1,2]. Such a dilemma in turn appeals to the development and employment of clean and renewable energy.展开更多
Brassinosteroids(BRs)play important roles in regulating plant reproductive processes.BR signaling or BR biosynthesis null mutants do not produce seeds under natural conditions,but the molecular mechanism underlying th...Brassinosteroids(BRs)play important roles in regulating plant reproductive processes.BR signaling or BR biosynthesis null mutants do not produce seeds under natural conditions,but the molecular mechanism underlying this infertility is poorly understood.In this study,we report that outer integument growth and embryo sac development were impaired in the ovules of the Arabidopsis thaliana BR receptor null mutant bri1-116.Gene expression and RNA-seq analyses showed that the expression of INNER NO OUTER(INO),an essential regulator of outer integument growth,was significantly reduced in the bri1-116 mutant.Increased INO expression due to overexpression or increased transcriptional activity of BRASSINAZOLE-RESISTANT 1(BZR1)in the mutant alleviated the outer integument growth defect in bri1-116 ovules,suggesting that BRs regulate outer integument growth partially via BZR1-mediated transcriptional regulation of INO.Meanwhile,INO expression in bzr-h,a null mutant for all BZR1 family genes,was barely detectable;and the outer integument of bzr-h ovules had much more severe growth defects than those of the bri1-116 mutant.Together,our findings establish a new role for BRs in regulating ovule development and suggest that BZR1 family transcription factors might regulate outer integument growth through both BRI1-dependent and BRI1-independent pathways.展开更多
基金supported by the National Natural Science Foundation of China(51972024,51702013,51902025)the Fundamental Research Funds for the Central Universities(FRF-BD-20-07A,2019NTST29)+1 种基金the Scientific and Technological Innovation Foundation of Shunde Graduate School,University of Science and Technology Beijing(BK19AE029)funding from China Scholarship Council。
文摘Over the past decades, the energy and concomitant environment issues, such as energy shortage, air pollution and global warming, have been becoming increasingly striking world-wide challenges [1,2]. Such a dilemma in turn appeals to the development and employment of clean and renewable energy.
基金This study wassupported by grants from the National Natural Science Foundation of China(91417313 and 2014CB943404 to W.T.)Department of Education of Hebei Province(LJRC015 to W.T.)the“one hundred talents project”of Hebei province(E2013100004 to Y.S.)。
文摘Brassinosteroids(BRs)play important roles in regulating plant reproductive processes.BR signaling or BR biosynthesis null mutants do not produce seeds under natural conditions,but the molecular mechanism underlying this infertility is poorly understood.In this study,we report that outer integument growth and embryo sac development were impaired in the ovules of the Arabidopsis thaliana BR receptor null mutant bri1-116.Gene expression and RNA-seq analyses showed that the expression of INNER NO OUTER(INO),an essential regulator of outer integument growth,was significantly reduced in the bri1-116 mutant.Increased INO expression due to overexpression or increased transcriptional activity of BRASSINAZOLE-RESISTANT 1(BZR1)in the mutant alleviated the outer integument growth defect in bri1-116 ovules,suggesting that BRs regulate outer integument growth partially via BZR1-mediated transcriptional regulation of INO.Meanwhile,INO expression in bzr-h,a null mutant for all BZR1 family genes,was barely detectable;and the outer integument of bzr-h ovules had much more severe growth defects than those of the bri1-116 mutant.Together,our findings establish a new role for BRs in regulating ovule development and suggest that BZR1 family transcription factors might regulate outer integument growth through both BRI1-dependent and BRI1-independent pathways.
