A trypsin-like molting-related serine protease cDNA （CfMRSP） was cloned from the spruce budworm, Choristoneurafumiferana. The full-length CfMRSP complementary DNA （cDNA） encoded a 43 kDa protein that contained a t...A trypsin-like molting-related serine protease cDNA （CfMRSP） was cloned from the spruce budworm, Choristoneurafumiferana. The full-length CfMRSP complementary DNA （cDNA） encoded a 43 kDa protein that contained a trypsin-like serine protease catalytic domain, but no clip domain. The C-terminal extension contained five cystein residues, which may allow the protein to form a homodimer through interchain disulfide bonds and regulate the activity of CfMRSE Phylogenetic tree analysis showed that CfMRSP clusters with lepidopteran homologues such as serine protease 1 of Lonomia obliqua, hemolymph proteinase 20 （HP20）, pattern recognition serine proteinase precursor （ProHP14） and a trypsin-like protein ofManduca sexta. Northern blot analysis of developmental expression of CfMRSP indicated that its transcripts were found primarily in the epidermis and were produced during all of the tested stadia, from 4th instar larvae to pupae, but increased levels of CfMRSP transcripts were always found after each molt. A high level of the protein was found in the epidermis by immunohistochemistry analysis. Altogether these data suggest that CfMRSP plays a role in the epidermis during molting and metamorphosis.展开更多
基金Acknowledgments This research was supported by the National Natural Science Foundation （Grant No.： 30571258） and the Guangdong Natural Science Foundation （Grant No.： 5005941） of China and Genome Canada Grant through the Ontario Genomics Institute of Canada.
文摘A trypsin-like molting-related serine protease cDNA （CfMRSP） was cloned from the spruce budworm, Choristoneurafumiferana. The full-length CfMRSP complementary DNA （cDNA） encoded a 43 kDa protein that contained a trypsin-like serine protease catalytic domain, but no clip domain. The C-terminal extension contained five cystein residues, which may allow the protein to form a homodimer through interchain disulfide bonds and regulate the activity of CfMRSE Phylogenetic tree analysis showed that CfMRSP clusters with lepidopteran homologues such as serine protease 1 of Lonomia obliqua, hemolymph proteinase 20 （HP20）, pattern recognition serine proteinase precursor （ProHP14） and a trypsin-like protein ofManduca sexta. Northern blot analysis of developmental expression of CfMRSP indicated that its transcripts were found primarily in the epidermis and were produced during all of the tested stadia, from 4th instar larvae to pupae, but increased levels of CfMRSP transcripts were always found after each molt. A high level of the protein was found in the epidermis by immunohistochemistry analysis. Altogether these data suggest that CfMRSP plays a role in the epidermis during molting and metamorphosis.
