The fruit of the acai palm （Euterpe oleracea Mart.） is a rich source of potential bioactive phytochemicals, however neither its precise composition nor the putative benefits to health from its consumption have been ...The fruit of the acai palm （Euterpe oleracea Mart.） is a rich source of potential bioactive phytochemicals, however neither its precise composition nor the putative benefits to health from its consumption have been fully characterised. This study aims to elucidate the composition of acai fruit pulp and to explore the potential of these extracts to confer protection in a cell culture based model of oxidative DNA damage. Extractions ofcai pulps were dissolved in a 80% methanol （methanol： water, 80：20 v/v） based solvent and the phytochemicals present in the extract were analysing by HPLC detector. Lipids were assessed via GC. The Folin-Ciocalteau assay was used to determine the purposes of providing a phytochemical rich food based comparator with established health benefits. Finally, protection against oxidative DNA damage was assessed in HT-29 cell lines exposed to hydrogen peroxide via the comet assay. Acai pulp extracts were found to be rich in flavonoids, anthocyanins and carotenoids. These extracts were found to protect against oxidative DNA damage （anti-genotoxicity）. A^ai extracts were more effective at preventing oxidative DNA damage than the blueberry extracts, although their antioxidant capacities as assessed by the FRAP assay were similar. These data shown that acai is a rich source of plant phytochemicals and that these chemicals may protect against oxidative stress in human colon cells （HT-29）. Further work is needed to establish the digestive fate of these phytochemicals and to prove the beneficial effects in human.展开更多
文摘The fruit of the acai palm （Euterpe oleracea Mart.） is a rich source of potential bioactive phytochemicals, however neither its precise composition nor the putative benefits to health from its consumption have been fully characterised. This study aims to elucidate the composition of acai fruit pulp and to explore the potential of these extracts to confer protection in a cell culture based model of oxidative DNA damage. Extractions ofcai pulps were dissolved in a 80% methanol （methanol： water, 80：20 v/v） based solvent and the phytochemicals present in the extract were analysing by HPLC detector. Lipids were assessed via GC. The Folin-Ciocalteau assay was used to determine the purposes of providing a phytochemical rich food based comparator with established health benefits. Finally, protection against oxidative DNA damage was assessed in HT-29 cell lines exposed to hydrogen peroxide via the comet assay. Acai pulp extracts were found to be rich in flavonoids, anthocyanins and carotenoids. These extracts were found to protect against oxidative DNA damage （anti-genotoxicity）. A^ai extracts were more effective at preventing oxidative DNA damage than the blueberry extracts, although their antioxidant capacities as assessed by the FRAP assay were similar. These data shown that acai is a rich source of plant phytochemicals and that these chemicals may protect against oxidative stress in human colon cells （HT-29）. Further work is needed to establish the digestive fate of these phytochemicals and to prove the beneficial effects in human.
