Objective:To evaluate in vitro effects of Tagetes minuta L. essential oil(TEO) on L3 Anisakis larvae type 1. Methods:In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae th...Objective:To evaluate in vitro effects of Tagetes minuta L. essential oil(TEO) on L3 Anisakis larvae type 1. Methods:In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae three different media were tested:1) a saline solution(SS); 2) an industrial marinating solution; 3) sunflower seeds oil(SO). For each media and concentrations of TEO(0.1%,0.5%,1.0% and 5.0% v/v),20 parasites were introduced into plastic Petri dishes(diameter 90 mm) and maintained at room temperature. As controls,larvae were maintained without TEO under identical experimental conditions in SS,MS and SO. A total of 900 larvae were tested. The normalized mean viability,LT100,LT50 and the percentage of inactivation at 24 hours were calculated. Results:In vitro tests revealed a complete inactivation of parasites in saline solution after 2 hours with 5% and 1% of TEO. In marinating solution,a complete inactivation of parasites was observed after 4 hours at all concentrations used. A slower activity for all TEO concentration was reported in SO. Conclusions:The results obtained,showing a strong activity against Anisakis larvae,confirm TEO as a larvicidal agent in the treatment of human anisakidosis and in the industrial marinating process.展开更多
Balantidium coli is a zoonotic protozoan parasite, and pork is considered the major source of Balantidium infection in humans. Transmission is direct and commonly occurs through the ingestion of water and food, especi...Balantidium coli is a zoonotic protozoan parasite, and pork is considered the major source of Balantidium infection in humans. Transmission is direct and commonly occurs through the ingestion of water and food, especially vegetables, contaminated with infectious cysts. Ingestion of meat contaminated, with faecal material during the evisceration process, can represent a potential risk of B. coli transmission. In order to determine the rate of B. coli infection in pigs regularly slaughtered at abattoirs in the province of Messina (Sicily, Italy), faecal samples of 242 pigs (122 commercial hybrid and 120 Nero Siciliano pigs) were collected and evaluated by standard methods for the presence of trophozoites and/or cysts. A total of 105 of the commercial hybrid (86.06%), but only 44 of the Nero Siciliano (36.66%) pigs, were identified as positive for B. coli infection. The results obtained, may be linked to the type of farming employed.展开更多
文摘Objective:To evaluate in vitro effects of Tagetes minuta L. essential oil(TEO) on L3 Anisakis larvae type 1. Methods:In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae three different media were tested:1) a saline solution(SS); 2) an industrial marinating solution; 3) sunflower seeds oil(SO). For each media and concentrations of TEO(0.1%,0.5%,1.0% and 5.0% v/v),20 parasites were introduced into plastic Petri dishes(diameter 90 mm) and maintained at room temperature. As controls,larvae were maintained without TEO under identical experimental conditions in SS,MS and SO. A total of 900 larvae were tested. The normalized mean viability,LT100,LT50 and the percentage of inactivation at 24 hours were calculated. Results:In vitro tests revealed a complete inactivation of parasites in saline solution after 2 hours with 5% and 1% of TEO. In marinating solution,a complete inactivation of parasites was observed after 4 hours at all concentrations used. A slower activity for all TEO concentration was reported in SO. Conclusions:The results obtained,showing a strong activity against Anisakis larvae,confirm TEO as a larvicidal agent in the treatment of human anisakidosis and in the industrial marinating process.
文摘Balantidium coli is a zoonotic protozoan parasite, and pork is considered the major source of Balantidium infection in humans. Transmission is direct and commonly occurs through the ingestion of water and food, especially vegetables, contaminated with infectious cysts. Ingestion of meat contaminated, with faecal material during the evisceration process, can represent a potential risk of B. coli transmission. In order to determine the rate of B. coli infection in pigs regularly slaughtered at abattoirs in the province of Messina (Sicily, Italy), faecal samples of 242 pigs (122 commercial hybrid and 120 Nero Siciliano pigs) were collected and evaluated by standard methods for the presence of trophozoites and/or cysts. A total of 105 of the commercial hybrid (86.06%), but only 44 of the Nero Siciliano (36.66%) pigs, were identified as positive for B. coli infection. The results obtained, may be linked to the type of farming employed.
