Surface-enhanced Raman scattering （SERS） has emerged as an excellent analytical tool for the effective detection and fingerprint identification of various chemicals. Recently, significant progress has been made in t...Surface-enhanced Raman scattering （SERS） has emerged as an excellent analytical tool for the effective detection and fingerprint identification of various chemicals. Recently, significant progress has been made in the fabrication of SERS-active substrates using simple, inexpensive, and affordable methods. The full potential of universal SERS diagnostics will likely be realized with the development of approaches and devices capable of effectively detecting analytes on various surfaces as well as in multicomponent media. In addition, the combination of implantable or wearable SERS-active substrates and remote portable devices enables real-time diagnostics that ideally fit the concept of personalized medicine. In this paper, we summarize recent achievements in fabricating flexible SERS substrates made of cellulose paper, polymer membranes, and textile fibrous films. Emphasis is placed on the in-situ extraction and detection of various chemicals in real-world surfaces and complex media using flexible nanofibrous SERS platforms. The potential SERS applications and future perspectives in on-site diagnostics are also discussed.展开更多
The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disa...The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.展开更多
文摘Surface-enhanced Raman scattering （SERS） has emerged as an excellent analytical tool for the effective detection and fingerprint identification of various chemicals. Recently, significant progress has been made in the fabrication of SERS-active substrates using simple, inexpensive, and affordable methods. The full potential of universal SERS diagnostics will likely be realized with the development of approaches and devices capable of effectively detecting analytes on various surfaces as well as in multicomponent media. In addition, the combination of implantable or wearable SERS-active substrates and remote portable devices enables real-time diagnostics that ideally fit the concept of personalized medicine. In this paper, we summarize recent achievements in fabricating flexible SERS substrates made of cellulose paper, polymer membranes, and textile fibrous films. Emphasis is placed on the in-situ extraction and detection of various chemicals in real-world surfaces and complex media using flexible nanofibrous SERS platforms. The potential SERS applications and future perspectives in on-site diagnostics are also discussed.
文摘The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.
