Ecological environment issues caused by soil erosion have always been the attractive and significant problems all over the world.Under the background of global warming,debris flow,landslide,and other intense gravitati...Ecological environment issues caused by soil erosion have always been the attractive and significant problems all over the world.Under the background of global warming,debris flow,landslide,and other intense gravitational erosion activities have become aggravated,which leads to the decrease of biological diversity,ecosystem stability,resistance,productivity,and the like,which presents new challenges to traditional measures of soil and water conservation.This article,based on research conducted on controlling mountain hazard on the Xiaojiang River basin over the last 30 years,summarizes the managerial achievement of typical ecological engineering technologies and analyzes the principles and application of each type of treatment.The results indicated that established ecological engineering technologies play a significant role in the prevention and treatment of intense gravitational erosion caused by mountain hazard.However,there are still a great deal of limitation of application condition and maneuverability during management process.How to furtherly develop the rational combining pattern between ecological engineering(e.g.contour hedgerow)and geotechnical engineering(e.g.slit dam)and how to strengthen the risk control and improve management strategy will be the key points for preventing intense gravitational erosion in future by ecological engineering.展开更多
The autonomous Mutator(Mu)transposon in maize(Zea mays L.)lines 115F,330I,and 715D were crossed with inbred lines B73,Mo17,97108,and H9-21,M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool ...The autonomous Mutator(Mu)transposon in maize(Zea mays L.)lines 115F,330I,and 715D were crossed with inbred lines B73,Mo17,97108,and H9-21,M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool and The M2plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the field.Next we statistically analyzed these candidate mutants.Under drought stress,we screened seedlings at 3-leaf stage by using the infrared thermography,and successfully got 108 droughtinsensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines,which temperature were significant different with others.These candidate mutants were primarily analyzed by infrared thermography,chlorophyll fluorescence,leaf water losing,photosynthetic characteristics,content of soluble sugars,soluble protein,and proline assay.The selected mutants were cloned by MuTAIL–PCR methods.Here we provide the better genetic materials for research on maize breeding for drought tolerance.展开更多
Cotton(Gossypium hirsutum L.)is a major crop and the main source of natural fiber worldwide.Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,improved stress resistance of t...Cotton(Gossypium hirsutum L.)is a major crop and the main source of natural fiber worldwide.Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,improved stress resistance of this crop plant is urgently needed.In this study,we used Gateway technology to construct a normalized full-length cDNA overexpressing(FOX)library from upland cotton cultivar ZM12 under various stress conditions.The library was transformed into Arabidopsis to produce a cotton-FOX-Arabidopsis library.Screening of this library yielded 6,830 transgenic Arabidopsis lines,of which 757 were selected for sequencing to ultimately obtain 659 cotton ESTs.GO and KEGG analyses mapped most of the cotton ESTs to plant biological process,cellular compo-nent,and molecular function categories.Next,156potential stress-responsive cotton genes were identified from the cotton-FOX-Arabidopsis library under drought,salt,ABA,and other stress conditions.Four stress-related genes identified from the library,designated as GhCAS,GhAPX,GhSDH,and GhPOD,were cloned from cotton complementary DNA,and their expression patterns under stress were analyzed.Phenotypic experiments in-dicated that overexpression of these cotton genes in Arabidopsis affected the response to abiotic stress.The method developed in this study lays a foundation for high-throughput cloning and rapid identification of cotton functional genes.展开更多
文摘Ecological environment issues caused by soil erosion have always been the attractive and significant problems all over the world.Under the background of global warming,debris flow,landslide,and other intense gravitational erosion activities have become aggravated,which leads to the decrease of biological diversity,ecosystem stability,resistance,productivity,and the like,which presents new challenges to traditional measures of soil and water conservation.This article,based on research conducted on controlling mountain hazard on the Xiaojiang River basin over the last 30 years,summarizes the managerial achievement of typical ecological engineering technologies and analyzes the principles and application of each type of treatment.The results indicated that established ecological engineering technologies play a significant role in the prevention and treatment of intense gravitational erosion caused by mountain hazard.However,there are still a great deal of limitation of application condition and maneuverability during management process.How to furtherly develop the rational combining pattern between ecological engineering(e.g.contour hedgerow)and geotechnical engineering(e.g.slit dam)and how to strengthen the risk control and improve management strategy will be the key points for preventing intense gravitational erosion in future by ecological engineering.
基金supported by the National Key Basic Research and Development Program of China(2012CB 114301)
文摘The autonomous Mutator(Mu)transposon in maize(Zea mays L.)lines 115F,330I,and 715D were crossed with inbred lines B73,Mo17,97108,and H9-21,M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool and The M2plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the field.Next we statistically analyzed these candidate mutants.Under drought stress,we screened seedlings at 3-leaf stage by using the infrared thermography,and successfully got 108 droughtinsensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines,which temperature were significant different with others.These candidate mutants were primarily analyzed by infrared thermography,chlorophyll fluorescence,leaf water losing,photosynthetic characteristics,content of soluble sugars,soluble protein,and proline assay.The selected mutants were cloned by MuTAIL–PCR methods.Here we provide the better genetic materials for research on maize breeding for drought tolerance.
基金This workwas supported by the National Key Research and Development Program of China(2016YFD0101900)the National Natural Science Foundation of China(31671728).
文摘Cotton(Gossypium hirsutum L.)is a major crop and the main source of natural fiber worldwide.Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,improved stress resistance of this crop plant is urgently needed.In this study,we used Gateway technology to construct a normalized full-length cDNA overexpressing(FOX)library from upland cotton cultivar ZM12 under various stress conditions.The library was transformed into Arabidopsis to produce a cotton-FOX-Arabidopsis library.Screening of this library yielded 6,830 transgenic Arabidopsis lines,of which 757 were selected for sequencing to ultimately obtain 659 cotton ESTs.GO and KEGG analyses mapped most of the cotton ESTs to plant biological process,cellular compo-nent,and molecular function categories.Next,156potential stress-responsive cotton genes were identified from the cotton-FOX-Arabidopsis library under drought,salt,ABA,and other stress conditions.Four stress-related genes identified from the library,designated as GhCAS,GhAPX,GhSDH,and GhPOD,were cloned from cotton complementary DNA,and their expression patterns under stress were analyzed.Phenotypic experiments in-dicated that overexpression of these cotton genes in Arabidopsis affected the response to abiotic stress.The method developed in this study lays a foundation for high-throughput cloning and rapid identification of cotton functional genes.
