Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explo...Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds,Min and Enshi black(ES)pigs,utilizing H3K27ac CUT&Tag,RNA-seq,and selective signature analysis.Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure.Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation,whereas those in the fat of ES pig are primarily associated with immunity-related genes.Moreover,transcription factors PRDM1 and HSF1,which show evidence of selection,are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat,respectively.Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs,providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.展开更多
Purpose As a fourth-generation light source,High Energy Photon Source(HEPS)has much more stringent requirements to the beam orbit stability in both horizontal and vertical directions than the previous sources due to t...Purpose As a fourth-generation light source,High Energy Photon Source(HEPS)has much more stringent requirements to the beam orbit stability in both horizontal and vertical directions than the previous sources due to the much smaller beam sizes.Methods A Fast Orbit FeedBack(FOFB)system,with the closed-loop bandwidth around 500 Hz,is needed to meet the critical requirements.The latency of the FOFB system is the key to achieve these requirements.Results Proper design and implementation of the feedback logic to have low latency is illustrated in the paper.Calculation accuracy is kept in the whole operation except for the last-minute data translation into 32-bit floating point number.展开更多
基金supported by the National Natural Science Foundation of China(32202634)National Natural Science Foundation of China(31961143020)+2 种基金National Natural Science Foundation of China(U20A2052)China Agricultural Research System(CARS-35)the Heilongjiang Provincial Research Institutes Research Business Fund Project(CZKYF2023-1-B009)。
文摘Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds,Min and Enshi black(ES)pigs,utilizing H3K27ac CUT&Tag,RNA-seq,and selective signature analysis.Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure.Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation,whereas those in the fat of ES pig are primarily associated with immunity-related genes.Moreover,transcription factors PRDM1 and HSF1,which show evidence of selection,are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat,respectively.Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs,providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.
基金This work is supported by Youth Innovation Promotion Association(Y9291420K2).
文摘Purpose As a fourth-generation light source,High Energy Photon Source(HEPS)has much more stringent requirements to the beam orbit stability in both horizontal and vertical directions than the previous sources due to the much smaller beam sizes.Methods A Fast Orbit FeedBack(FOFB)system,with the closed-loop bandwidth around 500 Hz,is needed to meet the critical requirements.The latency of the FOFB system is the key to achieve these requirements.Results Proper design and implementation of the feedback logic to have low latency is illustrated in the paper.Calculation accuracy is kept in the whole operation except for the last-minute data translation into 32-bit floating point number.
