In the present study, bacterial strains were isolated from the shrimp （Penaeus orientalis） ponds in different regions of Hainan Province, and tested for their antagonistic activity against Vibrio species on 2216E pl...In the present study, bacterial strains were isolated from the shrimp （Penaeus orientalis） ponds in different regions of Hainan Province, and tested for their antagonistic activity against Vibrio species on 2216E plates, using V. parahaemolyticus, V. harveyi and V. anguillarum as the indicator bacteria. As a result, an antagonistic strain was screened out and numbered 20160522Z-10 （referred to as Z-10）. Then, the antibacterial activity and minimum inhibitory concentration （MIG） of Z-10 against the indicator bacteria, and its antibacterial effect against other six Vibrio species were determined. The results proved that Z-10 was antagonistic to all the three indicator bacteria, and its MIC against V. parahaemolyticus, V. harveyi and V. anguillarum was 2.5× 10^4, 2.5× 10^5 and 2.5× 10^5 CFU/ml. Additionally, Z-10 had a certain antibacterial effect against other Vibrio species. Finally, the strain was identified as a Pseudoalteromonas sp. by 16S rDNA sequence analysis. The bacterium as a new probiotic, is expected to be used in disease prevention and control in marine aquaculture.展开更多
The precise localization of organic molecules in controllable positions is an important step towards constructing functional nanostructures via the bottom-up strategy. Herein, supramolecularly organized C70-fullerene ...The precise localization of organic molecules in controllable positions is an important step towards constructing functional nanostructures via the bottom-up strategy. Herein, supramolecularly organized C70-fullerene assemblies on macrocycle-modified surfaces were investigated using scanning tunneling microscopy （STM） in combination with theoretical calculations. The results revealed that an up-assembly of C70-fullerene adlayers was successfully formed on top of the bottom macrocycle arrays. Density functional theory （DFT） calculations confirmed that the macrocycle networks along with the co-adsorbed solvent 1-phenyloctane served as a selective template for trapping C70-fullerene molecules in the spectral sites and acted as a support for the C70-fullerene molecules. The periodical distribution of the C70-fullerene molecules should facilitate understanding of the strong dependence of the arrangement of C70-fullerene upon the specific interactions （apart from spatial recognition） derived from modification of the sub-monolayers.展开更多
基金Supported by Technology Development Program of Scientific Research Institutes of Hainan Province(11-20410-0010,11-20410-0009)
文摘In the present study, bacterial strains were isolated from the shrimp （Penaeus orientalis） ponds in different regions of Hainan Province, and tested for their antagonistic activity against Vibrio species on 2216E plates, using V. parahaemolyticus, V. harveyi and V. anguillarum as the indicator bacteria. As a result, an antagonistic strain was screened out and numbered 20160522Z-10 （referred to as Z-10）. Then, the antibacterial activity and minimum inhibitory concentration （MIG） of Z-10 against the indicator bacteria, and its antibacterial effect against other six Vibrio species were determined. The results proved that Z-10 was antagonistic to all the three indicator bacteria, and its MIC against V. parahaemolyticus, V. harveyi and V. anguillarum was 2.5× 10^4, 2.5× 10^5 and 2.5× 10^5 CFU/ml. Additionally, Z-10 had a certain antibacterial effect against other Vibrio species. Finally, the strain was identified as a Pseudoalteromonas sp. by 16S rDNA sequence analysis. The bacterium as a new probiotic, is expected to be used in disease prevention and control in marine aquaculture.
基金This work was supported by the National Basic Research Program of China （Nos. 2016YFA0200700, 2013CB934200, and 2012CB933001） and the National Natural Science Foundation of China （No. 21472029）.
文摘The precise localization of organic molecules in controllable positions is an important step towards constructing functional nanostructures via the bottom-up strategy. Herein, supramolecularly organized C70-fullerene assemblies on macrocycle-modified surfaces were investigated using scanning tunneling microscopy （STM） in combination with theoretical calculations. The results revealed that an up-assembly of C70-fullerene adlayers was successfully formed on top of the bottom macrocycle arrays. Density functional theory （DFT） calculations confirmed that the macrocycle networks along with the co-adsorbed solvent 1-phenyloctane served as a selective template for trapping C70-fullerene molecules in the spectral sites and acted as a support for the C70-fullerene molecules. The periodical distribution of the C70-fullerene molecules should facilitate understanding of the strong dependence of the arrangement of C70-fullerene upon the specific interactions （apart from spatial recognition） derived from modification of the sub-monolayers.
