Detection of canine echinococcosis by coproantigen ELISA

Objective:To study the canine echinococcosis by coproantigen ELISA method.Methods:During the present investigation experimental infection was established using evaginated worms of Echinococcus granulosus(E.granulosus).To check cross reactivity two pups were infected with Taenia hydatigena(T.hydatigena).In order to detect the presence of antigen,hyperimmune sera were raised against excretory-secretory products of adult worms E.chinococcus granulosus. Faecal sample collected either from experimentally infected pups or from other sources were heated at 70℃to detect heat stable soluble antigen.Results:Pups harbouring less than 104 worms showed negative results.Samples collected from 14 days onwards from experimentally infected animals harbouring more than 104 worms showed positive value.The maximum positive samples were detected in samples collected from in and around slaughter house and the least number of samples were detected positive maintained by dog squad.Conclusions:The affinity purified IgG exhibited promising results for detection of canine echinococcosis by indirect ELISA.

独立于铁代谢,雌性对铁超负荷心肌病有保护作用:氧化应激的关键作用

人类和实验动物中存在心脏功能和铁代谢的性别相关差异。男性患者和雄性动物模型更易患心肌病和心力衰竭。然而，在铁超负荷性心肌病中是否存在类似差异并不清楚。方法和结果：雄性和雌性野生型小鼠和铁调素调节蛋白（hemojuvelin）缺陷小鼠分别注射并高铁喂食，以诱导继发性铁超负荷和遗传性血色素沉着症。观察到雌性小鼠被保护免于发生铁超负荷性心肌病，而雄性铁超负荷小鼠发生明显的舒张功能障碍（基于超声心动图和侵入性压力一容积分析）。

对氨基苯甲酸为添加剂的Sonogashira反应

卤代芳烃以醋酸钯（1mol％）为催化剂，对氨基苯甲酸（2mol％）为添加剂，碳酸钾（3倍摩尔量）为碱，与末端炔烃（1．2倍摩尔量）在乙醇中室温或60℃发生Sonogashira反应生成交叉偶联产物，20例收率20％～97％。炔烃为苯乙炔时，卤代芳烃卤素间位、对位有吸电子基可提高反应收率。