Three-dimensional(3D)bioprinting based on traditional 3D printing is an emerging technology that is used to precisely assemble biocompatible materials and cells or bioactive factors into advanced tissue engineering so...Three-dimensional(3D)bioprinting based on traditional 3D printing is an emerging technology that is used to precisely assemble biocompatible materials and cells or bioactive factors into advanced tissue engineering solutions.Similar technology,particularly photo-cured bioprinting strategies,plays an important role in the field of tissue engineering research.The successful implementation of 3D bioprinting is based on the properties of photopolymerized materials.Photocrosslinkable hydrogel is an attractive biomaterial that is polymerized rapidly and enables process control in space and time.Photopolymerization is frequently initiated by ultraviolet(UV)or visible light.However,UV light may cause cell damage and thereby,affect cell viability.Thus,visible light is considered to be more biocompatible than UV light for bioprinting.In this review,we provide an overview of photo curing-based bioprinting technologies,and describe a visible light crosslinkable bioink,including its crosslinking mechanisms,types of visible light initiator,and biomedical applications.We also discuss existing challenges and prospects of visible light-induced 3D bioprinting devices and hydrogels in biomedical areas.展开更多
Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials,the onset and resolution of chronic inflammation,and macrophage polarization.However,the neutrophil response to biomaterials ...Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials,the onset and resolution of chronic inflammation,and macrophage polarization.However,the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood.Here,we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair.We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard,soft,naturally-and synthetically-derived materials.The overall trend showed increased neutrophil survival on naturally derived constructs,together with higher oxidative burst,decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials.The culture model is a step to better understand the immune modulation elicited by biomaterials.Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.展开更多
基金supported by the Key-Area Research and Development Program of Guangdong Province(2019B010941001)the Shenzhen Double Chain Project for Innovation and Development Industry supported by the Bureau of Industry and Information Technology of Shenzhen(201908141541)Shenzhen Fundamental Research Foundation(GJHZ20170314154845576 and GJHS20170314161106706).
文摘Three-dimensional(3D)bioprinting based on traditional 3D printing is an emerging technology that is used to precisely assemble biocompatible materials and cells or bioactive factors into advanced tissue engineering solutions.Similar technology,particularly photo-cured bioprinting strategies,plays an important role in the field of tissue engineering research.The successful implementation of 3D bioprinting is based on the properties of photopolymerized materials.Photocrosslinkable hydrogel is an attractive biomaterial that is polymerized rapidly and enables process control in space and time.Photopolymerization is frequently initiated by ultraviolet(UV)or visible light.However,UV light may cause cell damage and thereby,affect cell viability.Thus,visible light is considered to be more biocompatible than UV light for bioprinting.In this review,we provide an overview of photo curing-based bioprinting technologies,and describe a visible light crosslinkable bioink,including its crosslinking mechanisms,types of visible light initiator,and biomedical applications.We also discuss existing challenges and prospects of visible light-induced 3D bioprinting devices and hydrogels in biomedical areas.
文摘Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials,the onset and resolution of chronic inflammation,and macrophage polarization.However,the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood.Here,we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair.We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard,soft,naturally-and synthetically-derived materials.The overall trend showed increased neutrophil survival on naturally derived constructs,together with higher oxidative burst,decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials.The culture model is a step to better understand the immune modulation elicited by biomaterials.Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.
