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Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair 被引量:2
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作者 Longhou Fang YiGuo Wang +6 位作者 Dan Du Guang Yang Tim Tak Kwok Siu Kai Kong Benjamin chen david j chen Zhengjun chen 《Cell Research》 SCIE CAS CSCD 2007年第2期100-116,共17页
分区有缺陷者 3 (Par3 ) ,在 conservedPar3/Par6/aPKC 建筑群的一个关键部件,在房间极性的戏基础角色。此处,我们报导 Ku70 和通过试管内绑定试金的蛋白质由液体层析双人脚踏车质谱法跟随了的 Ku80 同样新奇的交往 Par3 的鉴定。Ku7... 分区有缺陷者 3 (Par3 ) ,在 conservedPar3/Par6/aPKC 建筑群的一个关键部件,在房间极性的戏基础角色。此处,我们报导 Ku70 和通过试管内绑定试金的蛋白质由液体层析双人脚踏车质谱法跟随了的 Ku80 同样新奇的交往 Par3 的鉴定。Ku70/Ku80 蛋白质是 DNA 依赖的蛋白激酶(DNA-PK ) 的二个关键规章的子单元,它在修理双海滨脱氧核糖核酸裂缝(DSB ) 起一个必要作用。我们决定 Par3 withKu70/Ku80 的原子协会被 y 照耀(红外) 提高,有势力 DSB inducer。而且, DNA-PKcs, DNA-PK 的催化子单元,响应红外与 Par3/Ku70/Ku80 建筑群交往了。Par3over 表示或击倒分别地能够 up- 或 downregulat-ing DNA-PK 活动。而且, Par3 击倒的房间被发现在随机的原生质标志集成有缺点,在 DSB 修理追随者红外有缺点、对射线敏感,类似于 Ku70knockdown 房间的显型。这些调查结果作为 DNA-PK 建筑群的一个新奇部件识别 Par3 并且含有到 DSB 修理的房间极性的一个意外连接。 展开更多
关键词 细胞极性蛋白 DNA双股断裂 DNA修复 DNA-PK Ku70/Ku80/Par3复合物
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Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair 被引量:1
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作者 Longhou Fangt YiGuo Wang +6 位作者 Dan Dut Guang Yang Tim Tak Kwok Siu Kai Kong Benjamin chen david j chen Zhengjun chen 《Cell Research》 SCIE CAS CSCD 2007年第6期572-574,共3页
The author affiliations were mixed up in the previous published version. The third fund number of National NaturalScience Foundation of China in the Acknowledgments was wrong, it should be "30270335". The Sh... The author affiliations were mixed up in the previous published version. The third fund number of National NaturalScience Foundation of China in the Acknowledgments was wrong, it should be "30270335". The Shanghai MunicipalCouncil for Science and Technology (No.06DZ22032) was missed in the Acknowledgments. There are some labelingand production errors in Figure 2A, Figure 3B and 3C, Figure 5C, Figure 6B and 6E, Figure 7B and 7D. In Figure 2A,left panel "A431" should be "Par3". In Figure 3B and 3C, "anti-Par3CT" should be "anti-Par3LCT", "GST-Par3CT"should be "GST-Par3LCT". In Figure 5C, the second arrow indicating "Lamin B" should be "[3-tubulin". In Figure 6Bright panel, the molecular weight for ?-actin should be "43" instead of"200". In Figure 6E, "Par3" should be "Par3i". Themolecular weight for the DNA-PKcs panel should be the same as the p-DNA-PKcs. In Figure 7B, the time point "240"in the left panel should be "120"; in the right panel of Figure 7B, the title for the y axis should be "DNA released (%)".In Figure 7D, the title for the y axis should be "Survival (%)", and the scale for the y axis should be "100, 10 and 1". These corrections do not affect the conclusions of the study. We apologize for any inconvenience this may havecaused. 展开更多
关键词 双链断裂修复 细胞极性蛋白质 细胞生物学 DNA-PK
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A special issue on DNA damage responses and genome maintenance
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作者 Guo-Min Li david j chen +1 位作者 Sankar Mitra john j Turchi 《Cell Research》 SCIE CAS CSCD 2008年第1期1-2,共2页
关键词 DNA 细胞 核糖核酸 基因 生物
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