Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases.The hydroethanolic extract(PME)from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models.Howe...Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases.The hydroethanolic extract(PME)from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models.However,its effects in chronic anxiety models and possible mechanism(s)of action were not studied.Therefore,the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety.The zebrafish light dark test(LDT)and novel tank test(NTT)were employed to assess the anxiolytic-like effects of PME(0.1,0.3,1.0 mg mL^(−1)),fluox-etine(3×10^(−5)mg mL^(−1))and diazepam(1.5×10^(−7)mg mL^(−1)).The chronic unpredictable stress(CUS)test was used to further evaluate the extract’s anxiolytic-like properties.The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil,granisetron,methysergide,or pizotifen,all at 1×10^(−3)mg mL^(−1).The extract significantly decreased anxiety behaviours in the NT and LD tests.These observed effects of the extract were however counteracted by flumazenil,granisetron,methysergide and pizotifen pre-treatment.In addition,PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish.Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.展开更多
文摘Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases.The hydroethanolic extract(PME)from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models.However,its effects in chronic anxiety models and possible mechanism(s)of action were not studied.Therefore,the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety.The zebrafish light dark test(LDT)and novel tank test(NTT)were employed to assess the anxiolytic-like effects of PME(0.1,0.3,1.0 mg mL^(−1)),fluox-etine(3×10^(−5)mg mL^(−1))and diazepam(1.5×10^(−7)mg mL^(−1)).The chronic unpredictable stress(CUS)test was used to further evaluate the extract’s anxiolytic-like properties.The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil,granisetron,methysergide,or pizotifen,all at 1×10^(−3)mg mL^(−1).The extract significantly decreased anxiety behaviours in the NT and LD tests.These observed effects of the extract were however counteracted by flumazenil,granisetron,methysergide and pizotifen pre-treatment.In addition,PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish.Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.
