The peptide primary messengers regulate embryonic development, cell growth and many other activities in animal cells. But recent evidence verified that peptide primary messengers are also involved in plant defense res...The peptide primary messengers regulate embryonic development, cell growth and many other activities in animal cells. But recent evidence verified that peptide primary messengers are also involved in plant defense responses, the recognition between pollen and stigma and keep the balance between cell proliferation and differentiations in shoot apical meristems. Those results suggest that plants may actually make wide use of peptide primary messengers, both in embryonic development and late life when they rally their cells to defend against pathogens and insect pests. The recent advance in those aspects is reviewed.展开更多
IN recent years, calmodulin （CaM）, an important Ca<sup>2+</sup> receptor and constituent of cellular signal transduction systems, has been found extracellularly. We have verified that CaM is presented ex...IN recent years, calmodulin （CaM）, an important Ca<sup>2+</sup> receptor and constituent of cellular signal transduction systems, has been found extracellularly. We have verified that CaM is presented extracellularly in all of plant species we have examined. In addition, we have reported that extracellular CaM has some biological significance, such as stimulation of cell proliferation, cell wall regeneration, initiation of pollen germination and tube growth and inducement of rbcS gene expression. The role of heterotrimeric G proteins in pollen germination, tube growth and signal transduction of extracellular CaM has been examined in Lily pollen, and two kinds of antibodies against animal Gzα internal sequence and N-terminal展开更多
The effects of calmodulin (CaM) antagonist W7_agarose, anti_CaM serum and exogenous purified CaM on pollen germination and tube growth of Forsythia suspensa were studied. The pollen germination and tube growth were in...The effects of calmodulin (CaM) antagonist W7_agarose, anti_CaM serum and exogenous purified CaM on pollen germination and tube growth of Forsythia suspensa were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7_agarose. The addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum albumin (BSA) had no effect. The inhibitory effects caused by W7_agarose and anti_CaM serum could be reversed completely by the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7_agarose. The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM enhances the above processes.展开更多
基金the National KeyBasic Research Special Funds of China (Grant No. G1999011700), the National Natural Science Foundation of China (Grant Nos. 39730230 and 39870365), Excellent Teacher Foundation of Education Department of China and Outstanding Younger Sc
文摘The peptide primary messengers regulate embryonic development, cell growth and many other activities in animal cells. But recent evidence verified that peptide primary messengers are also involved in plant defense responses, the recognition between pollen and stigma and keep the balance between cell proliferation and differentiations in shoot apical meristems. Those results suggest that plants may actually make wide use of peptide primary messengers, both in embryonic development and late life when they rally their cells to defend against pathogens and insect pests. The recent advance in those aspects is reviewed.
文摘IN recent years, calmodulin （CaM）, an important Ca<sup>2+</sup> receptor and constituent of cellular signal transduction systems, has been found extracellularly. We have verified that CaM is presented extracellularly in all of plant species we have examined. In addition, we have reported that extracellular CaM has some biological significance, such as stimulation of cell proliferation, cell wall regeneration, initiation of pollen germination and tube growth and inducement of rbcS gene expression. The role of heterotrimeric G proteins in pollen germination, tube growth and signal transduction of extracellular CaM has been examined in Lily pollen, and two kinds of antibodies against animal Gzα internal sequence and N-terminal
文摘The effects of calmodulin (CaM) antagonist W7_agarose, anti_CaM serum and exogenous purified CaM on pollen germination and tube growth of Forsythia suspensa were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7_agarose. The addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum albumin (BSA) had no effect. The inhibitory effects caused by W7_agarose and anti_CaM serum could be reversed completely by the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7_agarose. The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM enhances the above processes.
