Hydrogen peroxide （H2O2） is generated in many metabolic processes. As a signaling molecule, H2O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there...Hydrogen peroxide （H2O2） is generated in many metabolic processes. As a signaling molecule, H2O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there are three catalase genes, CAT1, CAT2, and CAT3. The encoded catalases are predominately peroxisomal pro- teins and are critical for scavenging H2O2. Since CAT1 and CAT3 are linked on chromosome 1, it has been almost impossible to generate cat1/3 and cats1/2/3 mutants by traditional genetic tools. In this study, we constructed cat1/3 double mutants and cat1/22/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases. The cat1/ 2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditionscompared with wild-type and the cat2/3 double mutants. Transcriptome analysis showed a more profound tran- scriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants. These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses. In addition, expression of OXI1 （OXIDATIVE SIGNAL INDUCIBLE 1） and several MAPK cascade genes were changed dramatically in the catalase triple mutant, suggesting that H2O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.展开更多
Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To pr...Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization , we examined mRNA populations in differently_treated plumules of winter wheat \%(Triticum aestivum \%L. \%cv\% Yanda 1817) using mRNA differential display. One vernalization_ related cDNA clone \%(VRC), VRC54\%, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that \%VRC54\% was a novel vernalization_related clone found in higher plant which not only might play an important role in the floral induction in vernalization_requiring plants but also was different from the cold_acclimatized genes.展开更多
基金funded by the Foundation for Taishan Scholar from the People’s Government of Shandong Province (tshw20130962)National Natural Science Foundation of China (31470352, 31670073, and 31600204)+2 种基金Natural Science Foundation of Shandong Province (ZR2014CM002 and ZR2016CB05)China Postdoctoral Science Foundation (2017M612333)Research in the Zhao Laboratory was supported by the National Science Foundation (IOS-0721192 and IOS-1322796)
文摘Hydrogen peroxide （H2O2） is generated in many metabolic processes. As a signaling molecule, H2O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there are three catalase genes, CAT1, CAT2, and CAT3. The encoded catalases are predominately peroxisomal pro- teins and are critical for scavenging H2O2. Since CAT1 and CAT3 are linked on chromosome 1, it has been almost impossible to generate cat1/3 and cats1/2/3 mutants by traditional genetic tools. In this study, we constructed cat1/3 double mutants and cat1/22/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases. The cat1/ 2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditionscompared with wild-type and the cat2/3 double mutants. Transcriptome analysis showed a more profound tran- scriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants. These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses. In addition, expression of OXI1 （OXIDATIVE SIGNAL INDUCIBLE 1） and several MAPK cascade genes were changed dramatically in the catalase triple mutant, suggesting that H2O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.
文摘Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization , we examined mRNA populations in differently_treated plumules of winter wheat \%(Triticum aestivum \%L. \%cv\% Yanda 1817) using mRNA differential display. One vernalization_ related cDNA clone \%(VRC), VRC54\%, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that \%VRC54\% was a novel vernalization_related clone found in higher plant which not only might play an important role in the floral induction in vernalization_requiring plants but also was different from the cold_acclimatized genes.
