Cells are the basic unit of life.Electrochemical analysis of single cells/organelles is essential for uncovering the molecular mechanisms of physiological and pathological processes that are difficult to elucidate on ...Cells are the basic unit of life.Electrochemical analysis of single cells/organelles is essential for uncovering the molecular mechanisms of physiological and pathological processes that are difficult to elucidate on a larger scale.This paper provides an overview of the commonly used fabrication methods for micro/nanoelectrodes applied in the investigations of single cells/organelles as well as the corresponding electrochemical measurements over the last four years including extracellular measurement,combination of extra and intracellular measurement,intracellular reactive oxygen species and reactive nitrogen species(ROS/RNS)measurement,and isolated organelles measurement.展开更多
Ultrafiltration(UF) experiments were subjected to the concentration of Shiitake polyphenol(SP) with ultrasound(US) enhancement, by which the effects of the main parameters such as ultrasonic power, transmembrane press...Ultrafiltration(UF) experiments were subjected to the concentration of Shiitake polyphenol(SP) with ultrasound(US) enhancement, by which the effects of the main parameters such as ultrasonic power, transmembrane pressure, temperature and axial flow-rate on the membrane fluxes were analyzed, and then the SP of the ultrafiltrate after follow-up resin adsorption by HPLC was investigated. The results indicated that US could significantly enhance the membrane fluxes. The optimal conditions obtained was as follows: 0.60 W/cm2 US power, axial flow-rate of 80 L/h,operation temperature 30 ℃, transmembrane pressure 0.10 MPa. US increased the absorption capacity of the absorbent resin during the following stage. The HPLC analysis also showed that catechins were purified to some extent as the ultrafiltrate was treated by US and macroporous resin, indicating the technology of US enhancement coupled with UF showed the potential for concentration and purification of SP by absorbent resins.展开更多
Stretchable strain sensors play an increasingly important role in artificial intelligent devices.However,high-performanee strain sensors havebeen slowly developed owing to the harsh requirement of self-powered functio...Stretchable strain sensors play an increasingly important role in artificial intelligent devices.However,high-performanee strain sensors havebeen slowly developed owing to the harsh requirement of self-powered function,long cycle life and high resolution.Here,we report aself-powered stretchable graphene-ecoflex composite strain sensor based on photo-thermoelectric(PTE)effect induced electricity.Thedevice exhibits a high strain sensitivity of-0.056 In(nA)/%with strains ranged from 0%to 20%under 980 nm light illumination,where thestrain sensitivity can be found to in crease with in creasi ng light inte nsity.The strain sensor maintains outstanding dyn amic stability un derperiodic strains ranged from 0 to 100%in 100 cycles.The sensing resolution can be as high as 0.5%with both the response and recovery timeof less than 0.6 s.It can precisely monitor human joint motions and stretchable strains by implanting the device in pork.展开更多
基金funding from Fundamental Research Funds for the Central Universities(No.20720220014)the National Natural Science Foundation of China(No.22204134).
文摘Cells are the basic unit of life.Electrochemical analysis of single cells/organelles is essential for uncovering the molecular mechanisms of physiological and pathological processes that are difficult to elucidate on a larger scale.This paper provides an overview of the commonly used fabrication methods for micro/nanoelectrodes applied in the investigations of single cells/organelles as well as the corresponding electrochemical measurements over the last four years including extracellular measurement,combination of extra and intracellular measurement,intracellular reactive oxygen species and reactive nitrogen species(ROS/RNS)measurement,and isolated organelles measurement.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201303080)Hubei Agricultural Science&Technology Innovation Center Project(2014-620-007-001)Natural Science Foundation of Hubei Province of China(2013CFB053)
文摘Ultrafiltration(UF) experiments were subjected to the concentration of Shiitake polyphenol(SP) with ultrasound(US) enhancement, by which the effects of the main parameters such as ultrasonic power, transmembrane pressure, temperature and axial flow-rate on the membrane fluxes were analyzed, and then the SP of the ultrafiltrate after follow-up resin adsorption by HPLC was investigated. The results indicated that US could significantly enhance the membrane fluxes. The optimal conditions obtained was as follows: 0.60 W/cm2 US power, axial flow-rate of 80 L/h,operation temperature 30 ℃, transmembrane pressure 0.10 MPa. US increased the absorption capacity of the absorbent resin during the following stage. The HPLC analysis also showed that catechins were purified to some extent as the ultrafiltrate was treated by US and macroporous resin, indicating the technology of US enhancement coupled with UF showed the potential for concentration and purification of SP by absorbent resins.
基金This work was supported by the National Key R&D Program of China(No.2016YFA0202701)the National Natural Science Foundation of China(No.51472055)+4 种基金External Cooperation Program of BIC,Chinese Academy of Sciences(No.121411KYS820150028)the 2015 Annual Beijing Talents Fund(No.2015000021223ZK32)Qingdao National Laboratory for Marine Science and Technology(No.2017ASKJ01)the University of Chinese Academy of Sciences(No.Y8540XX2D2)the"thousands talents"program for the pioneer researcher and his innovation team,China.
文摘Stretchable strain sensors play an increasingly important role in artificial intelligent devices.However,high-performanee strain sensors havebeen slowly developed owing to the harsh requirement of self-powered function,long cycle life and high resolution.Here,we report aself-powered stretchable graphene-ecoflex composite strain sensor based on photo-thermoelectric(PTE)effect induced electricity.Thedevice exhibits a high strain sensitivity of-0.056 In(nA)/%with strains ranged from 0%to 20%under 980 nm light illumination,where thestrain sensitivity can be found to in crease with in creasi ng light inte nsity.The strain sensor maintains outstanding dyn amic stability un derperiodic strains ranged from 0 to 100%in 100 cycles.The sensing resolution can be as high as 0.5%with both the response and recovery timeof less than 0.6 s.It can precisely monitor human joint motions and stretchable strains by implanting the device in pork.
