Potential antibacterial activity of berberine against multi drug resistant enterovirulent Escherichia coli isolated from yaks(Poephagus grunniens) with haemorrhagic diarrhoea

Objective:To evaluate the antimicrobial efficacy of berberine,a plant alkaloid.Methods:Five multi-drug resistant（MDR） STEC/EPEC and five MDR ETEC isolates from yaks with haemorrhagic diarrhoea were selected for the study.Antibacterial activity of berberine was evaluated by broth dilution and disc diffusion methods.The binding kinetics of berberine to DNA and protein was also enumerated.Results:For both categories of enterovirulent Escherichia coli（E.roli） isolates, berberine displayed the antibaclerial effect in a dose dependent manner.The MIC50 of berberine chloride for STEC/EPEC isolates varied from 2.07μM to 3.6μM with a mean of（2.95±0.33）μM where as for ETEC strains it varied from 1.75 to 1.96μM with a mean of（1.87±0.03）μM. Berberine bind more tightly with double helix DNA with Bmax and Kd of（24.68±2.62） and（357.8±57.8）,respectively.Berberine reacted with protein in comparatively loose manner with Bmax and Kd of（18.9±3.83） and 【286.2±113.6),respectively.Conclusions:The results indicate clearly that berberine may serve as a good antibacterial against multi drug resistant E.coli.

使用风扇改善大型电动机的冷却性能

本文对大容量开启式电动机采用低风阻结构，和前扫式倾斜叶片的轴流电扇的冷却性能进行了研究。电动机轴流风扇的风阻比普通风扇产生气流的阻力要大得多。气流在叶片表面明显地分离，并产生很大的流体噪声。我们将电动机的内部风阻降低，采用了前扫式倾斜叶片轴流式风扇。通过风道实验确认，该组合件的流量与传统的通风结构和轴流风扇比较，明显地提高了。以该流量围绕着叶片流动进行过数字流体分析。空气沿叶片表面流动没有出现分流的现象。流量显象实验也观察到了这种现象。后来用一台低风阻结构和前扫式倾斜叶片的轴流式风扇的实际电动机进行实验表明，与传统的电机比较，冷却空气的流量提高了80％，定子绕组的平均温升降低了30％。因此冷却性能得到了大大的改善。