5A模式下主动循环呼吸技术训练在肺癌手术患者中的效果

目的分析基于5A[询问(Ask)、评估(Asses)、建议(Advice)、帮助(Assist)、安排随访(Arrange)]模式下主动循环呼吸技术训练在胸腔镜肺癌手术患者中的应用效果。方法选取安徽省胸科医院2023年5月至2023年8月收治的100例拟行胸腔镜手术治疗的肺癌患者,使用简单随机法分别纳入观察组(n=50)、对照组(n=50)。2组均接受常规围术期综合护理,观察组加用基于5A模式的主动循环呼吸技术训练。对比2组术后恢复情况、排痰量、并发症差异,记录2组术前、术后3个月心肺功能和呼吸困难度、运动功能变化。结果观察组胸管拔除时间、胸腔引流量及术后住院时间均较对照组更低(P<0.05)。观察组术后第1天、第2天、第3天排痰量均较对照组更低(P<0.05)。观察组术后并发症发生率较对照组更低(P<0.05)。2组术后3个月左室射血分数(left ventricular ejection fraction,LVEF)均较术前1 d升高,左室舒张末期内径(left ventricular end diastolic diameter,LVEDD)均下降,且第1秒用力呼气容积(forced expiratory volume for 1 second,FEV1)、用力肺活量(Forced vital capacity,FVC)、最大呼气流量(peak expiratory flow,PEF)均较术前1 d下降;观察组术后3个月LVEF较对照组更高、LVEDD较对照组更低,且FEV1、FVC、PEF均较对照组更高(P<0.05)。2组术后3个月呼吸困难量表(multiple myeloma research consortium,mMRC)评分均较术前1 d升高、6 min步行距离(6-min walking distance,6MWT)均下降;观察组术后3个月mMRC评分较对照组更低、6MWT较对照组更高(P<0.05)。结论基于5A模式下主动循环呼吸技术训练能够促进肺癌患者胸腔镜手术后早期恢复和术后排痰,也有助于预防术后并发症、促进术后运动功能改善,并有效降低术后呼吸困难度。

Genetic and Phenotypic Investigation of a Chinese Pedigree with Lattice Corneal Dystrophy ⅢB Subtype

Purpose:.To investigate phenotypes and disease-causing mutation in the transforming growth factor b-induced gene(TGFBI) in a Southern Chinese pedigree with lattice corneal dystrophy(LCD) IIIB with complicated cataract.Methods:.A Southern Chinese pedigree with lattice corneal dystrophy IIIB with complicated cataract was recruited. Comprehensive ophthalmic investigations were performed before and after cataract surgery of phacoemulsification and intraocular lens implantation in the proband's both eyes..Peripheral blood was collected from the proband,.and genomic DNA was extracted..All exons of the TGFBI gene were sequenced to screen possible mutations.Results:.A bilateral LCD IIIB subtype was observed in the proband..Optical coherence tomography further revealed superreflective changes in the subepithelial and stroma layers of the cornea,.with reduced central corneal thickness..Notably,bilateral cataract was found in the proband..Direct sequencing detected a recurrent heterozygous missense c.1877A>G mutation in exon 14 of the TGFBI gene,.resulting in substitution of histidine with arginine(p.H626R).Conclusion:.The current study was the first report of the TGFBI p.H626R mutation in Southern Chinese,.suggesting that it could be a mutation hotspot across populations..Moreover,.the mutation was associated with LCD IIIB subtype with complicated cataract,.which had not been reported before,pointing to clinical heterogeneity of the mutation.

Loss of the ubiquitin-conjugating enzyme Rad6B disturbs mitochondrial function and cellular homeostasis in mouse skin

Various factors can induce cell degeneration by altering the phenotype and metabolism of cells.Mitochondria play an essential role in cellular homeostasis and function,rendering aging processes highly associated with mitochondrial function and status.Herein,we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B(Ube2b),an E2 ubiquitin-conjugating enzyme.In this study,using Masson’s trichrome,we showed that loss of Rad6B causes physiological structure changes in mouse skin with age.In addition,a combination of western blotting experiments,transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of Rad6B was characterized by an abnormal mitochondrial metabolic profile,including decreased mitochondrial biosynthesis and mitochondrial complex activities,increased ROS production and suppressed mitophagy resulting in metabolic disorders.Taken together,these results highlight an important relationship between Rad6B and skin senescence,suggesting that Rad6B plays an indispensable role in maintaining a healthy mitochondrial network and intracellular homeostasis.

Calculation of the Gas Injection Rate and Pipe String Erosion in Nitrogen Drilling Systems

Detailed information is provided for the design and construction of nitrogen drilling in a coal seam.Two prototype wells are considered.The Guo model is used to calculate the required minimum gas injection rate,while the Finnie,Sommerfeld,and Tulsa models are exploited to estimate the ensuing erosion occurring in pipe strings.The calculated minimum gas injection rates are 67.4 m^(3)/min(with water)and 49.4 m^(3)/min(without water),and the actual field of use is 90–120 m^(3)/min.The difference between the calculated injection pressure and the field value is 6.5%–15.2%(formation with water)and 0.65%–7.32%(formation without water).The results show that the Guo model can more precisely represent the situation of the no water formation in the nitrogen drilling of a coal seam.The Finnie,Sommerfeld,and Tulsa models have different sensitivities to cutting densities,particle size,impact velocity and angle,and pipe string hardness.

Intravesical Treatment with Vorinostat Can Prevent Tumor Progression in MNU Induced Bladder Cancer

Background: Histone deacetylase inhibitors (HDACI) are promising class of drugs acting as antiproliferative agents by promoting differentiation as well as inducing apoptosis. Vorinostat (suberoylanilide hydroxamic acid, SAHA) is the first among this new class of anticancer drugs to be approved by FDA for the treatment of cancer but only for cutaneous T cell lymphoma (CTCL). The objective of this study is to investigate the inhibitory effect of SAHA on the viability of human bladder cancer cells and its synergetic effect with chemotherapy agents in vitro and in vivo. Methods: The cell viability of human bladder cancer cell lines after treated with SAHA or SAHA combining mitomycin c (MMC), Cisplatin (DDP) and Adriamycin (ADM) were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Hoechst staining was used to observe cell morphology for apoptotic cells. The survivin protein and acetylated histone H3 levels in bladder cancer were quantified by Western blot analysis. In vivo tumor growth inhibition of intravesical inject SAHA was determined in rats with N-methyl-N-nitrosourea (MNU) induced bladder cancer. Results: SAHA significantly inhibited growth of bladder cancer cell lines with concentration and time dependent manner. Furthermore, better results of tumor inhibition would be achieved when it was combined with chemotherapeutic agents in vitro and in vivo. Survivin expression decreased and acetylated histone H3 expression increased in bladder cancer cells lines after SAHA treatment. Intravesically injections of SAHA can inhibit tumor progress when combined with DDP. Conclusions: SAHA can act as HDACI which has direct anti-cancer effect and can enhance the action of several chemotherapy agents markedly. SAHA may sensitize bladder cancer to anti cancer drugs by down regulating survivin expression. Intravesically injections of SAHA can prevent tumor progression in MNU induced bladder cancer.

A Study on the Instructional Design of Interdisciplinary Thematic Learning in Middle School Physical Education Based on the CASES-T Model

With the deepening of educational reform,interdisciplinary thematic learning,as an emerging educational model,has become a focus of attention in the field of educational research.Based on the STEM(science,technology,engineering,and mathematics)education concept and CASES-T(Content,Activity,Situation,Evaluation,Strategy-Target)model,this study provides a theoretical basis for the teaching design and implementation of interdisciplinary thematic learning in middle school physical education.Through the analysis of specific interdisciplinary thematic learning cases,it aims to provide theoretical support and practical guidance for the reform of middle school physical education through the CASES-T model-based interdisciplinary thematic teaching design research in middle school physical education,in order to enhance students’learning effects,cultivate core literacy in physical education,and promote students’all-round development.

Histone post-translational modification and the DNA damage response

DNA is highly vulnerable to spontaneous and environmental timely damage in living cells.DNA damage may cause genetic instability and increase cancer risk if the damages are not repaired timely and efficiently.Human cells possess several DNA damage response(DDR)mechanisms to protect the integrity of their genome.Clarification of the mechanisms under-lying the DNA damage response following lethal damage will facilitate the identification of therapeutic targets for cancers.Histone post-translational modifications(PTMs)have been indicated to play different roles in the repair of DNA damage.In this context,histone PTMs regulate recruitment of downstream effectors,and facilitate appropriate repair response.This review outlines the current understanding of different histone PTMs in response to DNA dam-age repair,besides,enumerates the role of new type PTMs such as histone succinylation and crotonylation in regulating DNA damage repair processes.

Dual-wavelength Bi2Se3-based passively Q-switching Nd3+-doped glass all-fiber laser

We demonstrate a dual-wavelength passively Q-switched Nd^（3＋）-doped glass fiber laser using a few-layer topological insulator Bi2Se3 as a saturable absorber（SA） for the first time, to the best of our knowledge. The laser resonator is a simple and compact linear cavity using two fiber end-facet mirrors. The SA is fabricated by Bi2Se3/polyvinyl alcohol composite film. By inserting the SA into the laser cavity, a stable Q-switching operation is achieved with the shortest pulse width and maximum pulse repetition rate of 601 ns and 205.2 kHz,respectively. The maximum average output power and maximum pulse energy obtained are about 6.6 mW and 38.8 nJ, respectively.