WRKY transcription factors(TFs)are of great importance in plant responses to different abiotic stresses.However,research on their roles in the regulation of thermotolerance remains limited.Here,we investigated the fun...WRKY transcription factors(TFs)are of great importance in plant responses to different abiotic stresses.However,research on their roles in the regulation of thermotolerance remains limited.Here,we investigated the function of LlWRKY39 in the thermotolerance of lily(Lilium longiflorum‘white heaven’).According to multiple alignment analyses,LIWRKY39 is in the WRKY IId subclass and contains a potential calmodulin(CaM)-binding domain.Further analysis has shown that LlCaM3 interacts with LlWRKY39 by binding to its CaM-binding domain,and this interaction depends on Ca^(2).LIWRKY39 was induced by heat stress(HS),and the LIWRKY39-GFP fusion protein was detected in the nucleus.The thermotolerance of lily and Arabidopsis was increased with the ectopic overexpression of LlWRKY39.The expression of heat-related genes AtHSFA1,AtHSFA2,AtMBF1c,AtGolS1,AtDREB2A,AtWRKY39,and AtHSP101 was significantly elevated in transgenic Arabidopsis lines,which might have promoted an increase in thermotolerance.Then,the promoter of LlMBF1c was isolated from lily,and LlWRKY39 was found to bind to the conserved W-box element in its promoter to activate its activity,suggesting that LlWRKY39 is an upstream regulator of LlMBF1c.In addition,a dual-luciferase reporter assay showed that via protein interaction,LlCaM3 negatively affected LlWRKY39 in the transcriptional activation of LlMBF1c,which might be an important feedback regulation pathway to balance the LlWRKY39-mediated heat stress response(HSR).Collectively,these results imply that LlWRKY39 might participate in the HSR as an important regulator through Ca^(2+)-CaM and multiprotein bridging factor pathways.展开更多
In this analysis, the single crystal of schiff base has been synthesized and the purity of material has been increased by repeated recrystallization process. Single crystal was grown by adopting the method growing in ...In this analysis, the single crystal of schiff base has been synthesized and the purity of material has been increased by repeated recrystallization process. Single crystal was grown by adopting the method growing in a slow evaporation solution using ethanol as solvent at room temperature. A new fluorescent molecule based on Schiff base has been synthesised and its binding properties investigated by fluorescence spectroscopy to show that it can selectively bind Cu2+ with fluorescence quenching.展开更多
A pair of new anticancer nucleosides based on 1,2,4-triazole nucleosides and 1-((2-hydroxyethoxy) methyl)-5-(phenylthio)-1H-1,2,4-triazole-3-carboxamide have been synthesized, and have given the corresponding products...A pair of new anticancer nucleosides based on 1,2,4-triazole nucleosides and 1-((2-hydroxyethoxy) methyl)-5-(phenylthio)-1H-1,2,4-triazole-3-carboxamide have been synthesized, and have given the corresponding products in excellent yields. Its structures and conformations were confirmed by single crystal X-ray diffraction.展开更多
An important anticancer nucleosides intermediate (2R,3S,4S,5R)-2-(acetoxymethyl)-5-(3-bromo-5-(methoxycar-bonyl)-1H-1,2,4-triazol-1-yl)tetrahydrofuran-3,4-diyl diacetate was synthesized by directly coupling the bromot...An important anticancer nucleosides intermediate (2R,3S,4S,5R)-2-(acetoxymethyl)-5-(3-bromo-5-(methoxycar-bonyl)-1H-1,2,4-triazol-1-yl)tetrahydrofuran-3,4-diyl diacetate was synthesized by directly coupling the bromotriazole with the protected ribose sugar, and have given the corresponding product in moderate yield. Its structure and conformation were confirmed by single crystal X-ray diffraction.展开更多
In our previous study,a heat-induced differentially expressed WRKY-IIe gene LlWRKY22 is isolated from lily(Lilium longiflorum),which acts as a positive role in thermotolerance,but whether it is involved in other stres...In our previous study,a heat-induced differentially expressed WRKY-IIe gene LlWRKY22 is isolated from lily(Lilium longiflorum),which acts as a positive role in thermotolerance,but whether it is involved in other stress responses is unknown.Here,the expression of LlWRKY22 was indicated to be positively influenced by heat,salt,or mannitol treatments,and its promoter activity was also enhanced after heat,salt,or mannitol treatments.In addition,LlWRKY22 responded to ABA treatment,which activated its expression and also increased the promoter activity.Overexpression of LlWRKY22 in Arabidopsis contributed to growth defects and early flowering.Simultaneously,compared with the wild type,the ABA sensitivity in transgenic lines was increased in both the germination stage and late growth stage.Further analysis showed that LlWRKY22 overexpression elevated the thermotolerance of transgenic plants and induced the expression of AtDREB2A,AtDREB2B,AtDREB2C,and AtJUB1.The salt and mannitol tolerances of the overexpression lines were also improved.Overall,our results illustrated that LlWRKY22 is affected by heat,salt,and osmotic stresses,and positively regulates heat,salt,and osmotic tolerances,which reveals that it acts as a generalist character responding to different abiotic stresses.And further to that,the regulatory pathway of LlWRKY22 also involves in ABA signaling.展开更多
Lily was grown worldwide as a fresh cutting flower because of its colorful petals, but its anther contained a large number of pollen grains that cause serious pollen contamination, however, pollen abortion can effecti...Lily was grown worldwide as a fresh cutting flower because of its colorful petals, but its anther contained a large number of pollen grains that cause serious pollen contamination, however, pollen abortion can effectively reduce the level of pollen pollution. Our analysis aims to use cytological observation to detect the critical stage when pollen abortion occurs and to provide comprehensive gene expression information at the transcriptional level. The result showed that pollen abortion in ‘Little Kiss’ began at the mononuclear stage and the callose that covers the microspores failed to degenerate when young pollens were released from the tetrads. In addition, compared with the normally developed one,the tapetum of ‘Little Kiss’ degraded in advance while the degradation of callose was delayed. Furthermore, 103 differentially expressed genes(DEGs) related to the advance degeneration of tapetum cells and callose were found in the expression levels, including 22 transcription factors(TFs). In particular, two β-glucanase genes(endo-1,3(4)-β-glucanase, exo-β-glucanase) responsible for callose degeneration were significantly down-regulated. These results suggested that pollen abortion may occur at mononuclear stage and that early degeneration of tapetum cells resulted in a significant down-regulation of β-glucanase genes. As a result, the callose to cover microspores impedes the formation of pollen walls, which may possibly lead to pollen abortion.展开更多
基金the National Key R&D Program of China(Grant No.2019YFD1000400)the National Natural Science Foundation of China(31902055)+2 种基金the High Level Talent Project of the Top Six Talents in Jiangsu,China(NY-077)the Natural Science Foundation of Jiangsu Province,China(BK20190532)the Fundamental Research Funds for the Central Universities(KJQN202032)。
文摘WRKY transcription factors(TFs)are of great importance in plant responses to different abiotic stresses.However,research on their roles in the regulation of thermotolerance remains limited.Here,we investigated the function of LlWRKY39 in the thermotolerance of lily(Lilium longiflorum‘white heaven’).According to multiple alignment analyses,LIWRKY39 is in the WRKY IId subclass and contains a potential calmodulin(CaM)-binding domain.Further analysis has shown that LlCaM3 interacts with LlWRKY39 by binding to its CaM-binding domain,and this interaction depends on Ca^(2).LIWRKY39 was induced by heat stress(HS),and the LIWRKY39-GFP fusion protein was detected in the nucleus.The thermotolerance of lily and Arabidopsis was increased with the ectopic overexpression of LlWRKY39.The expression of heat-related genes AtHSFA1,AtHSFA2,AtMBF1c,AtGolS1,AtDREB2A,AtWRKY39,and AtHSP101 was significantly elevated in transgenic Arabidopsis lines,which might have promoted an increase in thermotolerance.Then,the promoter of LlMBF1c was isolated from lily,and LlWRKY39 was found to bind to the conserved W-box element in its promoter to activate its activity,suggesting that LlWRKY39 is an upstream regulator of LlMBF1c.In addition,a dual-luciferase reporter assay showed that via protein interaction,LlCaM3 negatively affected LlWRKY39 in the transcriptional activation of LlMBF1c,which might be an important feedback regulation pathway to balance the LlWRKY39-mediated heat stress response(HSR).Collectively,these results imply that LlWRKY39 might participate in the HSR as an important regulator through Ca^(2+)-CaM and multiprotein bridging factor pathways.
文摘In this analysis, the single crystal of schiff base has been synthesized and the purity of material has been increased by repeated recrystallization process. Single crystal was grown by adopting the method growing in a slow evaporation solution using ethanol as solvent at room temperature. A new fluorescent molecule based on Schiff base has been synthesised and its binding properties investigated by fluorescence spectroscopy to show that it can selectively bind Cu2+ with fluorescence quenching.
文摘A pair of new anticancer nucleosides based on 1,2,4-triazole nucleosides and 1-((2-hydroxyethoxy) methyl)-5-(phenylthio)-1H-1,2,4-triazole-3-carboxamide have been synthesized, and have given the corresponding products in excellent yields. Its structures and conformations were confirmed by single crystal X-ray diffraction.
文摘An important anticancer nucleosides intermediate (2R,3S,4S,5R)-2-(acetoxymethyl)-5-(3-bromo-5-(methoxycar-bonyl)-1H-1,2,4-triazol-1-yl)tetrahydrofuran-3,4-diyl diacetate was synthesized by directly coupling the bromotriazole with the protected ribose sugar, and have given the corresponding product in moderate yield. Its structure and conformation were confirmed by single crystal X-ray diffraction.
基金supported by the National Natural Science Foundation of China(31902055)the Fundamental Research Funds for the Central Universities(KYZZ2022004)+1 种基金the National Key R&D Program of China(2019YFD1000400)the Natural Science Foundation of Jiangsu Province,China(BK20190532).
文摘In our previous study,a heat-induced differentially expressed WRKY-IIe gene LlWRKY22 is isolated from lily(Lilium longiflorum),which acts as a positive role in thermotolerance,but whether it is involved in other stress responses is unknown.Here,the expression of LlWRKY22 was indicated to be positively influenced by heat,salt,or mannitol treatments,and its promoter activity was also enhanced after heat,salt,or mannitol treatments.In addition,LlWRKY22 responded to ABA treatment,which activated its expression and also increased the promoter activity.Overexpression of LlWRKY22 in Arabidopsis contributed to growth defects and early flowering.Simultaneously,compared with the wild type,the ABA sensitivity in transgenic lines was increased in both the germination stage and late growth stage.Further analysis showed that LlWRKY22 overexpression elevated the thermotolerance of transgenic plants and induced the expression of AtDREB2A,AtDREB2B,AtDREB2C,and AtJUB1.The salt and mannitol tolerances of the overexpression lines were also improved.Overall,our results illustrated that LlWRKY22 is affected by heat,salt,and osmotic stresses,and positively regulates heat,salt,and osmotic tolerances,which reveals that it acts as a generalist character responding to different abiotic stresses.And further to that,the regulatory pathway of LlWRKY22 also involves in ABA signaling.
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Fundamental Research Funds for the Central Universities(Grant No.KYTZ201602)+1 种基金the High Level Talent Project of the Top Six Talents in Jiangsu(Grant No.NY-077)the earmarked fund for germplasm resources of Nanjing Agricultural University(Grant No.KYZZ201920)。
文摘Lily was grown worldwide as a fresh cutting flower because of its colorful petals, but its anther contained a large number of pollen grains that cause serious pollen contamination, however, pollen abortion can effectively reduce the level of pollen pollution. Our analysis aims to use cytological observation to detect the critical stage when pollen abortion occurs and to provide comprehensive gene expression information at the transcriptional level. The result showed that pollen abortion in ‘Little Kiss’ began at the mononuclear stage and the callose that covers the microspores failed to degenerate when young pollens were released from the tetrads. In addition, compared with the normally developed one,the tapetum of ‘Little Kiss’ degraded in advance while the degradation of callose was delayed. Furthermore, 103 differentially expressed genes(DEGs) related to the advance degeneration of tapetum cells and callose were found in the expression levels, including 22 transcription factors(TFs). In particular, two β-glucanase genes(endo-1,3(4)-β-glucanase, exo-β-glucanase) responsible for callose degeneration were significantly down-regulated. These results suggested that pollen abortion may occur at mononuclear stage and that early degeneration of tapetum cells resulted in a significant down-regulation of β-glucanase genes. As a result, the callose to cover microspores impedes the formation of pollen walls, which may possibly lead to pollen abortion.