DYF371 locus and single‑nucleotide polymorphisms(SNPs)defined as DYF371C or DYF371T were simultaneously examined for 142 Chinese unrelated male individuals,including 90 Han population and 52 minorities.In total,37 SNP...DYF371 locus and single‑nucleotide polymorphisms(SNPs)defined as DYF371C or DYF371T were simultaneously examined for 142 Chinese unrelated male individuals,including 90 Han population and 52 minorities.In total,37 SNP-short tandem repeat haplotypes were detected.Nine of these 37 haplotypes were unique(24.32%).The gene diversity and discrimination capacity values are 0.8321 and 0.2606,respectively.Furthermore,only five males(5.55%)in the Han samples were determined that they had one copy of allele with SNP T-type and three copies of alleles with SNP C-types,whereas 14 individuals(26.92%)were observed in minorities’samples.The genotype proportion comprising three distinguishing copies of the allele with SNP C-types in Han sample was greatly lower than that in the sample of minorities.Similar results were shown for allele 11C and 10C.Therefore,the alleles of DYF371 with SNP C-type have the potential to differentiate the Han and non-Han Chinese populations.展开更多
Two rare cases of long alleles at Y‑chromosome short tandem repeat(Y‑STR)loci(DYF387S1 and DYS447)were identified when two father-son pairs were analyzed by multiplex amplification.“Null alleles”were observed at DYF...Two rare cases of long alleles at Y‑chromosome short tandem repeat(Y‑STR)loci(DYF387S1 and DYS447)were identified when two father-son pairs were analyzed by multiplex amplification.“Null alleles”were observed at DYF387S1 and DYS447,and duplicated alleles were displayed at DYS533 and DYS19.We secondly amplified DYF387S1,DYS533,DYS447,and DYS19 loci by singleplex polymerase chain reaction(PCR)and sequence analysis of the long alleles at DYF387S1 and DYS447 loci.The results showed that alleles from DYF387S1(allele 55)and DYS447(allele 41)were longer than their common sizes in the allelic ladder ranges(33-42 for DYF387S1 and 18-30 for DYS447)and located in the neighboring loci(DYS533 and DYS19,respectively).Therefore,to identify these cases involving this unusual phenomenon,not only re‑amplification using the same kit but also additional amplification(using alternative multiplex kits with different adjoining markers or additional singleplex PCR amplification)should be performed to avoid misinterpreting Y‑STR profiles.展开更多
文摘DYF371 locus and single‑nucleotide polymorphisms(SNPs)defined as DYF371C or DYF371T were simultaneously examined for 142 Chinese unrelated male individuals,including 90 Han population and 52 minorities.In total,37 SNP-short tandem repeat haplotypes were detected.Nine of these 37 haplotypes were unique(24.32%).The gene diversity and discrimination capacity values are 0.8321 and 0.2606,respectively.Furthermore,only five males(5.55%)in the Han samples were determined that they had one copy of allele with SNP T-type and three copies of alleles with SNP C-types,whereas 14 individuals(26.92%)were observed in minorities’samples.The genotype proportion comprising three distinguishing copies of the allele with SNP C-types in Han sample was greatly lower than that in the sample of minorities.Similar results were shown for allele 11C and 10C.Therefore,the alleles of DYF371 with SNP C-type have the potential to differentiate the Han and non-Han Chinese populations.
文摘Two rare cases of long alleles at Y‑chromosome short tandem repeat(Y‑STR)loci(DYF387S1 and DYS447)were identified when two father-son pairs were analyzed by multiplex amplification.“Null alleles”were observed at DYF387S1 and DYS447,and duplicated alleles were displayed at DYS533 and DYS19.We secondly amplified DYF387S1,DYS533,DYS447,and DYS19 loci by singleplex polymerase chain reaction(PCR)and sequence analysis of the long alleles at DYF387S1 and DYS447 loci.The results showed that alleles from DYF387S1(allele 55)and DYS447(allele 41)were longer than their common sizes in the allelic ladder ranges(33-42 for DYF387S1 and 18-30 for DYS447)and located in the neighboring loci(DYS533 and DYS19,respectively).Therefore,to identify these cases involving this unusual phenomenon,not only re‑amplification using the same kit but also additional amplification(using alternative multiplex kits with different adjoining markers or additional singleplex PCR amplification)should be performed to avoid misinterpreting Y‑STR profiles.
