To identify quantitative trait loci （QTLs） controlling panicle architecture in japonica rice, a genetic map was constructed based on simple sequence repeat （SSR） markers and 254 recombinant inbred lines （RILs） d...To identify quantitative trait loci （QTLs） controlling panicle architecture in japonica rice, a genetic map was constructed based on simple sequence repeat （SSR） markers and 254 recombinant inbred lines （RILs） derived from a cross between cultivars Xiushui 79 and C Ban. Seven panicle traits were investigated under three environments. Single marker analysis indicated that a total of 27 SSR markers were highly associated with panicle traits in all the three environments. Percentage of phenotypic variation explained by single locus varied from 2% to 35%. Based on the mixed linear model, a total of 40 additive QTLs for seven panicle traits were detected by composite interval mapping, explaining 1.2%--35% ofphenotypic variation. Among the 9 QTLs with more than 10% of explained phenotypic variation, two QTLs were for the number of primary branches per panicle （NPB）, two for panicle length （PL）, two for spikelet density （SD）, one for the number of secondary branches per panicle （NSB）, one for secondary branch distribution density （SBD）, and one for the num- ber of spikelets per panicle （NS）, respectively, qPLSD-9-1 and qPLSD-9-2 were novel pleiotropic loci, showing effects on PL and SD simultaneously, qPLSD-9-1 explained 34.7% of the phenotypic variation for PL and 25.4% of the phenotypic variation for SD, respec- tively, qPLSD-9-2 explained 34.9% and 24.4% of the phenotypic variation for PL and SD, respectively. The C Bao alleles at the both QTLs showed positive effects on PL, and the Xiushui 79 alleles at the both QTLs showed positive effects on SD. Genetic variation of panicle traits are mainly attributed to additive effects. QTLx environment interactions were not significant for additive QTLs and additive x additive QTL pairs.展开更多
基金supported by the Program for Changjiang Scholars and Innovative Research Team of Nanjing Agriculture University (No. IRT0432)
文摘To identify quantitative trait loci （QTLs） controlling panicle architecture in japonica rice, a genetic map was constructed based on simple sequence repeat （SSR） markers and 254 recombinant inbred lines （RILs） derived from a cross between cultivars Xiushui 79 and C Ban. Seven panicle traits were investigated under three environments. Single marker analysis indicated that a total of 27 SSR markers were highly associated with panicle traits in all the three environments. Percentage of phenotypic variation explained by single locus varied from 2% to 35%. Based on the mixed linear model, a total of 40 additive QTLs for seven panicle traits were detected by composite interval mapping, explaining 1.2%--35% ofphenotypic variation. Among the 9 QTLs with more than 10% of explained phenotypic variation, two QTLs were for the number of primary branches per panicle （NPB）, two for panicle length （PL）, two for spikelet density （SD）, one for the number of secondary branches per panicle （NSB）, one for secondary branch distribution density （SBD）, and one for the num- ber of spikelets per panicle （NS）, respectively, qPLSD-9-1 and qPLSD-9-2 were novel pleiotropic loci, showing effects on PL and SD simultaneously, qPLSD-9-1 explained 34.7% of the phenotypic variation for PL and 25.4% of the phenotypic variation for SD, respec- tively, qPLSD-9-2 explained 34.9% and 24.4% of the phenotypic variation for PL and SD, respectively. The C Bao alleles at the both QTLs showed positive effects on PL, and the Xiushui 79 alleles at the both QTLs showed positive effects on SD. Genetic variation of panicle traits are mainly attributed to additive effects. QTLx environment interactions were not significant for additive QTLs and additive x additive QTL pairs.
