Diaphorina citri is an important vector of Citrus Huanglongbing(HLB)disease.After feeding on young host plant shoots,the population of D.citri can increase significantly.Females also only lay eggs on young shoots.Howe...Diaphorina citri is an important vector of Citrus Huanglongbing(HLB)disease.After feeding on young host plant shoots,the population of D.citri can increase significantly.Females also only lay eggs on young shoots.However,there are few studies on the mechanism of this phenomenon.Exogenous nutrient signals can affect the insulin signaling system of D.citri after feeding on young shoots.In this study,the expression of upstreamfactors DclLPl,DclLP2,and DclR in the insulin signaling system of D.citri was upregulated after feeding on young shoots.After being silenced by RNA interference technology,the results showed that the number of oviposited eggs of D.citri was significantly decreased and the ovarian development was inhibited with severe vacuolation.In addition,detection using quantitative reverse transcription-polymerase chain reaction showed that the upstream regulatory gene DcRheb of the target of rapamycin(TOR)pathway and the downstream reproduction-related DcVg gene were also significantly downregulated.These results suggest that feeding upon young shoots may upregulate the expression levels of upstream factors DclLPl,DclLP2,and DclR in the insulin signaling system.The signal will be through upregulating the expression of DcRheb,an upstream gene of the TOR signaling pathway.This in turn influences yolk metabolism,which eventually causes the ovaries of female D.citri to mature and therefore initiate oviposition behavior.展开更多
基金supported by the Guangdong Laboratory of Lingnan Modern Agriculture Project(NT2021003)Natural Science Foundation of Guangdong Province(2019A1515011062)+3 种基金NSFC-Guangdong Joint Research Fund(U1701231)the National High Level Talent Special Support Plan(2020)the Earmarked Fund for Guangdong Modern Agro-Industry Technology Research System(2022KJ108)Study on Control Technology of Huanglongbing(KY-2017-001).
文摘Diaphorina citri is an important vector of Citrus Huanglongbing(HLB)disease.After feeding on young host plant shoots,the population of D.citri can increase significantly.Females also only lay eggs on young shoots.However,there are few studies on the mechanism of this phenomenon.Exogenous nutrient signals can affect the insulin signaling system of D.citri after feeding on young shoots.In this study,the expression of upstreamfactors DclLPl,DclLP2,and DclR in the insulin signaling system of D.citri was upregulated after feeding on young shoots.After being silenced by RNA interference technology,the results showed that the number of oviposited eggs of D.citri was significantly decreased and the ovarian development was inhibited with severe vacuolation.In addition,detection using quantitative reverse transcription-polymerase chain reaction showed that the upstream regulatory gene DcRheb of the target of rapamycin(TOR)pathway and the downstream reproduction-related DcVg gene were also significantly downregulated.These results suggest that feeding upon young shoots may upregulate the expression levels of upstream factors DclLPl,DclLP2,and DclR in the insulin signaling system.The signal will be through upregulating the expression of DcRheb,an upstream gene of the TOR signaling pathway.This in turn influences yolk metabolism,which eventually causes the ovaries of female D.citri to mature and therefore initiate oviposition behavior.
