Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show tha...Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show that Yunnan walnut has a great expectation on temperature and moisture.Temperature,including average temperature in January,average temperature in July,the average annual temperature and accumulated temperature≥10℃,has the greatest impact on the distribution of Yunnan walnut,and is the primary dominant factor;moisture,including annual rainfall and average relative humidity,has a great impact on the distribution of Yunnan walnut,and is the secondary dominant factor.展开更多
[ Objective] This study aimed to optimize the ISSR-PCR reaction system and select polymorphic ISSR primers for Olea euyopaea. [Method] O. euyo- paea genomic DNA was extracted from leaves as the template for optimizati...[ Objective] This study aimed to optimize the ISSR-PCR reaction system and select polymorphic ISSR primers for Olea euyopaea. [Method] O. euyo- paea genomic DNA was extracted from leaves as the template for optimization of ISSR-PCR reaction system by single-factor experiments on the main factors including Mg2＋ , dNTPs, primer concentration and template amount. [ Result ] The optimal ISSR-PCR reaction system for O. euyopaea was obtained, with a total system vol- ume of 20μl containing 1 × Taq buffer, 3.5 mmol/L Mg2＋ , 0.4 mmol/L dNTPs, 1.0 μmol/L primers, 1.0 U of Taq DNA polymerase and 20 ng of DNA tem- plate. The optimal ISSR-PCR reaction program was started with predenaturation at 94 ℃ for 5 min, followed by 40 cycles of denaturation at 94 ℃ for 30 s, annea- ling at 52 - 55 ℃ for 30 s, and extension at 72 ℃ for 2 min ; the amplification was completed by holding the reaction mixture at 72 ℃ for 10 min to allow complete extension of PCR products. PCR products were stored at 4 ℃. Based on the above conditions, 11 primers with high polymorphism, clear amplified bands and good repeatability were selected. [ Conclusion ] This study laid the foundation for further diversity research and species identification of O. euyopaea germplasm展开更多
基金Supported by National Science and Technology Support Program(2011BAD46B01-1)Yunnan Applied Basic Research Project(2012FD080)
文摘Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show that Yunnan walnut has a great expectation on temperature and moisture.Temperature,including average temperature in January,average temperature in July,the average annual temperature and accumulated temperature≥10℃,has the greatest impact on the distribution of Yunnan walnut,and is the primary dominant factor;moisture,including annual rainfall and average relative humidity,has a great impact on the distribution of Yunnan walnut,and is the secondary dominant factor.
基金Supported by Project of Important New Product Development in Yunnan Province ( 2009BB006)
文摘[ Objective] This study aimed to optimize the ISSR-PCR reaction system and select polymorphic ISSR primers for Olea euyopaea. [Method] O. euyo- paea genomic DNA was extracted from leaves as the template for optimization of ISSR-PCR reaction system by single-factor experiments on the main factors including Mg2＋ , dNTPs, primer concentration and template amount. [ Result ] The optimal ISSR-PCR reaction system for O. euyopaea was obtained, with a total system vol- ume of 20μl containing 1 × Taq buffer, 3.5 mmol/L Mg2＋ , 0.4 mmol/L dNTPs, 1.0 μmol/L primers, 1.0 U of Taq DNA polymerase and 20 ng of DNA tem- plate. The optimal ISSR-PCR reaction program was started with predenaturation at 94 ℃ for 5 min, followed by 40 cycles of denaturation at 94 ℃ for 30 s, annea- ling at 52 - 55 ℃ for 30 s, and extension at 72 ℃ for 2 min ; the amplification was completed by holding the reaction mixture at 72 ℃ for 10 min to allow complete extension of PCR products. PCR products were stored at 4 ℃. Based on the above conditions, 11 primers with high polymorphism, clear amplified bands and good repeatability were selected. [ Conclusion ] This study laid the foundation for further diversity research and species identification of O. euyopaea germplasm
