Establishment of Agrobacterium tumefaciens-mediated Genetic Transformation System for Aspergillus awamori

[ Objective ] This study aimed to establish Agrobacterium tumefaciens-mediated genetic transformation system for Aspergillus awamori and investigate the feasibility of expressing heterologous proteins in A. awamori. [ Method] Appropriate A. awamori host strains were determined according to the secretory protein profile. Selectable marker was selected for genetic transformation by drug sensitivity analysis. The established A. awamori genetic transformation system was used for transformation and expression analysis of Rhizomucor miehei lipase （RML）. The feasibility of using A. awamori to express heterologous proteins was investigated by identification of transformants and property analysis. [ Result~ Based on the analysis of secretory protein profile, A. awamori strains CBS115.52 and CICC2257 were determined as the host strains for heterologous protein expression ; drug sensitivity analysis shows that hygromycin B resistance gene （ HygBr ） is an effective ge- netic seleetable marker; by using Agrobacterium tumefaciens-mediatcd transformation （ATMT） method, the plasmid pHGW-amdS containing HygBr was successfully transformed into A. awamori strain CBS115.52 to establish the genetic transformation system ofA. awamorl with HygBr as selectable marker. RML was transformed into A. awamori and its expression was validated by substrate hydrolysis test, SDS-PAGE and Western blot. [ Conclusion] This study demonstrates that the genetic transformation system of A. awamori mediated by Agrobacterium tumefaciens has potential feasibility for expression of heterologous proteins.

A Comparative Study on Alternative Education in China and Korea

Alternative educational practice has something in common that has been raised as an alternative to the crisis of traditional public education,but the causes,like background,and development patterns of alternative education may be different.As an alternative to restore education,various progressive schools or programs have been operated in Korea and China.The purpose of this study was to shed light on the alternative education in Korea and China,and seek to research its development process and practice.

Dewey and Tao Xingzhi’s Educational Philosophy and Contemporary Interpretation

Tao Xingzhi was a student of American educator John Dewey.He generally absorbed Dewey’s educational philosophy in educational theory and practice,and carried out reforms and innovations based on the actual situation in China.Thus he formed his own peculiar“life education”theory.There are fundamental differences between Tao Xingzhi and Dewey in educational philosophy.Tao Xingzhi’s educational thought is based on real life and realistic materialistic educational view;Dewey’s educational thought is an idealistic educational thought based on personal life and subjective experience.The comparison of Tao Xingzhi’s and Dewey’s educational concepts has important practical significance for whether Tao Xingzhi’s educational concept can be accurately evaluated and used for reference in practice.The international education competition in the 21st century is not only the competition of economic forces,but also the competition of social productive forces.Essentially,it’s a technological power race.However,the key to rapidly transforming technology into productivity is universal access to education.

Boundedness of some multipliers on stratified Lie groups

Let ￡ be the sub-Laplacian on a stratified Lie group G, and let m be a function defined on [0, ＋∞）. We give the boundedness of the multiplier operators m（￡） on Herz-type Hardy spaces on G.

Powerful supercontinuum vortices generated by femtosecond vortex beams with thin plates

We demonstrate numerically and experimentally the generation of powerful supercontinuum vortices from femtosecond vortex beams by using multiple thin fused silica plates.The supercontinuum vortices are shown to preserve the vortex phase profile of the initial beam for spectral components ranging from 500 nm to 1200 nm.The transfer of the vortex phase profile results from the inhibition of multiple filamentation and the preservation of the vortex ring with relatively uniform intensity distribution by means of the thin-plate scheme,where the supercontinuum is mainly generated from the self-phase modulation and self-steepening effects.Our scheme works for vortex beams with different topological charges,which provides a simple and effective method to generate supercontinuum vortices with high power.